Differing Dietary Nutrients and Diet-Associated Bacteria Has Limited Impact on Spider Gut Microbiota Composition

Spiders are a key predator of insects across ecosystems and possess great potential as pest control agents. Unfortunately, it is difficult to artificially cultivate multiple generations of most spider species. Since gut bacterial flora has been shown to significantly alter nutrient availability, it...

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Autores principales:	Wang Zhang, Fengjie Liu, Yang Zhu, Runhua Han, Letian Xu, Jie Liu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	gut microbiota spider diet regime ants nutrient diet-associated microbes Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/46fd6e38748e49e08ae67052bbc9af63
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spelling	oai:doaj.org-article:46fd6e38748e49e08ae67052bbc9af632021-11-25T18:25:24ZDiffering Dietary Nutrients and Diet-Associated Bacteria Has Limited Impact on Spider Gut Microbiota Composition10.3390/microorganisms91123582076-2607https://doaj.org/article/46fd6e38748e49e08ae67052bbc9af632021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2358https://doaj.org/toc/2076-2607Spiders are a key predator of insects across ecosystems and possess great potential as pest control agents. Unfortunately, it is difficult to artificially cultivate multiple generations of most spider species. Since gut bacterial flora has been shown to significantly alter nutrient availability, it is plausible that the spiders’ microbial community plays a key role in their unsuccessful breeding. However, both the gut microbial composition and its influencing factors in many spiders remain a mystery. In this study, the gut microbiota of <i>Campanicola campanulata</i>, specialists who prey on ants and are widely distributed across China, was characterized. After, the impact of diet and diet-associated bacteria on gut bacterial composition was evaluated. First, two species of prey ants (<i>Lasius niger</i> and <i>Tetramorium caespitum</i>) were collected from different locations and fed to <i>C. campanulata.</i> For each diet, we then profiled the nutritional content of the ants, as well as the bacterial communities of both the ants and spiders. Results showed that the protein and carbohydrate content varied between the two prey ant species. We isolated 682 genera from 356 families in the ants (dominant genera including <i>Pseudomonas</i>, <i>Acinetobacter</i>, <i>Paraburkholderia</i>, <i>Staphylococcus,</i> and <i>Novosphingobium</i>), and 456 genera from 258 families in the spiders (dominated by <i>Pseudomonas</i>). However, no significant differences were found in the gut microbiota of spiders that were fed the differing ants. Together, these results indicate that nutritional variation and diet-associated bacterial differences have a limited impact on the microbial composition of spider guts, highlighting that spiders may have a potentially stable internal environment and lay the foundation for future investigations into gut microbiota.Wang ZhangFengjie LiuYang ZhuRunhua HanLetian XuJie LiuMDPI AGarticlegut microbiotaspiderdiet regimeantsnutrientdiet-associated microbesBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2358, p 2358 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gut microbiota spider diet regime ants nutrient diet-associated microbes Biology (General) QH301-705.5
spellingShingle	gut microbiota spider diet regime ants nutrient diet-associated microbes Biology (General) QH301-705.5 Wang Zhang Fengjie Liu Yang Zhu Runhua Han Letian Xu Jie Liu Differing Dietary Nutrients and Diet-Associated Bacteria Has Limited Impact on Spider Gut Microbiota Composition
description	Spiders are a key predator of insects across ecosystems and possess great potential as pest control agents. Unfortunately, it is difficult to artificially cultivate multiple generations of most spider species. Since gut bacterial flora has been shown to significantly alter nutrient availability, it is plausible that the spiders’ microbial community plays a key role in their unsuccessful breeding. However, both the gut microbial composition and its influencing factors in many spiders remain a mystery. In this study, the gut microbiota of <i>Campanicola campanulata</i>, specialists who prey on ants and are widely distributed across China, was characterized. After, the impact of diet and diet-associated bacteria on gut bacterial composition was evaluated. First, two species of prey ants (<i>Lasius niger</i> and <i>Tetramorium caespitum</i>) were collected from different locations and fed to <i>C. campanulata.</i> For each diet, we then profiled the nutritional content of the ants, as well as the bacterial communities of both the ants and spiders. Results showed that the protein and carbohydrate content varied between the two prey ant species. We isolated 682 genera from 356 families in the ants (dominant genera including <i>Pseudomonas</i>, <i>Acinetobacter</i>, <i>Paraburkholderia</i>, <i>Staphylococcus,</i> and <i>Novosphingobium</i>), and 456 genera from 258 families in the spiders (dominated by <i>Pseudomonas</i>). However, no significant differences were found in the gut microbiota of spiders that were fed the differing ants. Together, these results indicate that nutritional variation and diet-associated bacterial differences have a limited impact on the microbial composition of spider guts, highlighting that spiders may have a potentially stable internal environment and lay the foundation for future investigations into gut microbiota.
format	article
author	Wang Zhang Fengjie Liu Yang Zhu Runhua Han Letian Xu Jie Liu
author_facet	Wang Zhang Fengjie Liu Yang Zhu Runhua Han Letian Xu Jie Liu
author_sort	Wang Zhang
title	Differing Dietary Nutrients and Diet-Associated Bacteria Has Limited Impact on Spider Gut Microbiota Composition
title_short	Differing Dietary Nutrients and Diet-Associated Bacteria Has Limited Impact on Spider Gut Microbiota Composition
title_full	Differing Dietary Nutrients and Diet-Associated Bacteria Has Limited Impact on Spider Gut Microbiota Composition
title_fullStr	Differing Dietary Nutrients and Diet-Associated Bacteria Has Limited Impact on Spider Gut Microbiota Composition
title_full_unstemmed	Differing Dietary Nutrients and Diet-Associated Bacteria Has Limited Impact on Spider Gut Microbiota Composition
title_sort	differing dietary nutrients and diet-associated bacteria has limited impact on spider gut microbiota composition
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/46fd6e38748e49e08ae67052bbc9af63
work_keys_str_mv	AT wangzhang differingdietarynutrientsanddietassociatedbacteriahaslimitedimpactonspidergutmicrobiotacomposition AT fengjieliu differingdietarynutrientsanddietassociatedbacteriahaslimitedimpactonspidergutmicrobiotacomposition AT yangzhu differingdietarynutrientsanddietassociatedbacteriahaslimitedimpactonspidergutmicrobiotacomposition AT runhuahan differingdietarynutrientsanddietassociatedbacteriahaslimitedimpactonspidergutmicrobiotacomposition AT letianxu differingdietarynutrientsanddietassociatedbacteriahaslimitedimpactonspidergutmicrobiotacomposition AT jieliu differingdietarynutrientsanddietassociatedbacteriahaslimitedimpactonspidergutmicrobiotacomposition
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Differing Dietary Nutrients and Diet-Associated Bacteria Has Limited Impact on Spider Gut Microbiota Composition

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