Dehydroevodiamine Suppresses Inflammatory Responses in Adjuvant-Induced Arthritis Rats and Human Fibroblast-Like Synoviocytes

Dehydroevodiamine (DHE) is an effective natural active substance extracted from Euodiae Fructus, which is a widely used herbal drug in traditional Chinese medicine. The focus of this study was to test the possibility of using DHE in the treatment of rheumatoid arthritis (RA) diseases. A rat model of...

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Autores principales:	Yufang Dai, Jiaoe Sheng, Sanshan He, Qingchao Wu, Yunlong Wang, Linchong Su
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	dehydroevodiamine rheumatoid arthritis aia mh7a mapk Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/46ff79bc8f834721bea9b338ea699c59
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spelling	oai:doaj.org-article:46ff79bc8f834721bea9b338ea699c592021-11-04T15:51:54ZDehydroevodiamine Suppresses Inflammatory Responses in Adjuvant-Induced Arthritis Rats and Human Fibroblast-Like Synoviocytes2165-59792165-598710.1080/21655979.2021.1999554https://doaj.org/article/46ff79bc8f834721bea9b338ea699c592021-10-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1999554https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Dehydroevodiamine (DHE) is an effective natural active substance extracted from Euodiae Fructus, which is a widely used herbal drug in traditional Chinese medicine. The focus of this study was to test the possibility of using DHE in the treatment of rheumatoid arthritis (RA) diseases. A rat model of adjuvant-induced arthritis (AIA) was generated using Complete Freund’s Adjuvant (CFA). Body weight changes, arthritis scores, ankle pathology, tumor necrosis factor-alpha (TNF-α), interleukin-1β(IL-1β), interleukin-6 (IL-6), and interleukin-17 (IL-17) secretion, as well as matrix metalloproteinase (MMP) expression in joint tissue, were measured as indicators of viability of DHE medicated AIA rats. Human fibroblast-like synoviocytes (MH7A cells) were connected to check these impacts. The results confirmed that DHE administration had an excellent therapeutic impact on the AIA rat model, substantially relieving joint swelling, inhibiting synovial pannus hyperplasia, and decreasing joint scores. In addition, the serum enzyme-linked immunosorbent assay (ELISA) showed that DHE treatment reduced the expression of pro-inflammatory factors in AIA rats. The immunohistochemical results showed that DHE treatment could reduce the synthesis of MMPs such as matrix metalloproteinase-1(MMP-1) and matrix metalloproteinase-3 (MMP-3) in the ankle tissue of AIA rats. In vitro, DHE inhibited cell proliferation, mRNA transcription, protein synthesis of proinflammatory factors such as IL-1βand IL-6, and matrix metalloproteinases such as MMP-1 and MMP-3. Furthermore, DHE inhibited the phosphorylation levels of p38, JNK, and ERK proteins in TNF-α-treated MH7A cells.This work assessed the effect of DHE in AIA rats and revealed its mechanism in vitro.Yufang DaiJiaoe ShengSanshan HeQingchao WuYunlong WangLinchong SuTaylor & Francis Grouparticledehydroevodiaminerheumatoid arthritisaiamh7amapkBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dehydroevodiamine rheumatoid arthritis aia mh7a mapk Biotechnology TP248.13-248.65
spellingShingle	dehydroevodiamine rheumatoid arthritis aia mh7a mapk Biotechnology TP248.13-248.65 Yufang Dai Jiaoe Sheng Sanshan He Qingchao Wu Yunlong Wang Linchong Su Dehydroevodiamine Suppresses Inflammatory Responses in Adjuvant-Induced Arthritis Rats and Human Fibroblast-Like Synoviocytes
description	Dehydroevodiamine (DHE) is an effective natural active substance extracted from Euodiae Fructus, which is a widely used herbal drug in traditional Chinese medicine. The focus of this study was to test the possibility of using DHE in the treatment of rheumatoid arthritis (RA) diseases. A rat model of adjuvant-induced arthritis (AIA) was generated using Complete Freund’s Adjuvant (CFA). Body weight changes, arthritis scores, ankle pathology, tumor necrosis factor-alpha (TNF-α), interleukin-1β(IL-1β), interleukin-6 (IL-6), and interleukin-17 (IL-17) secretion, as well as matrix metalloproteinase (MMP) expression in joint tissue, were measured as indicators of viability of DHE medicated AIA rats. Human fibroblast-like synoviocytes (MH7A cells) were connected to check these impacts. The results confirmed that DHE administration had an excellent therapeutic impact on the AIA rat model, substantially relieving joint swelling, inhibiting synovial pannus hyperplasia, and decreasing joint scores. In addition, the serum enzyme-linked immunosorbent assay (ELISA) showed that DHE treatment reduced the expression of pro-inflammatory factors in AIA rats. The immunohistochemical results showed that DHE treatment could reduce the synthesis of MMPs such as matrix metalloproteinase-1(MMP-1) and matrix metalloproteinase-3 (MMP-3) in the ankle tissue of AIA rats. In vitro, DHE inhibited cell proliferation, mRNA transcription, protein synthesis of proinflammatory factors such as IL-1βand IL-6, and matrix metalloproteinases such as MMP-1 and MMP-3. Furthermore, DHE inhibited the phosphorylation levels of p38, JNK, and ERK proteins in TNF-α-treated MH7A cells.This work assessed the effect of DHE in AIA rats and revealed its mechanism in vitro.
format	article
author	Yufang Dai Jiaoe Sheng Sanshan He Qingchao Wu Yunlong Wang Linchong Su
author_facet	Yufang Dai Jiaoe Sheng Sanshan He Qingchao Wu Yunlong Wang Linchong Su
author_sort	Yufang Dai
title	Dehydroevodiamine Suppresses Inflammatory Responses in Adjuvant-Induced Arthritis Rats and Human Fibroblast-Like Synoviocytes
title_short	Dehydroevodiamine Suppresses Inflammatory Responses in Adjuvant-Induced Arthritis Rats and Human Fibroblast-Like Synoviocytes
title_full	Dehydroevodiamine Suppresses Inflammatory Responses in Adjuvant-Induced Arthritis Rats and Human Fibroblast-Like Synoviocytes
title_fullStr	Dehydroevodiamine Suppresses Inflammatory Responses in Adjuvant-Induced Arthritis Rats and Human Fibroblast-Like Synoviocytes
title_full_unstemmed	Dehydroevodiamine Suppresses Inflammatory Responses in Adjuvant-Induced Arthritis Rats and Human Fibroblast-Like Synoviocytes
title_sort	dehydroevodiamine suppresses inflammatory responses in adjuvant-induced arthritis rats and human fibroblast-like synoviocytes
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/46ff79bc8f834721bea9b338ea699c59
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_version_	1718444712196571136

Dehydroevodiamine Suppresses Inflammatory Responses in Adjuvant-Induced Arthritis Rats and Human Fibroblast-Like Synoviocytes

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