Single-cell CAS-seq reveals a class of short PIWI-interacting RNAs in human oocytes

Single-cell CAS-seq reveals a class of short PIWI-interacting RNAs in human oocytes

PIWI-interacting RNAs (piRNAs) are ~25–33 nt small RNAs expressed in animal germ cells. Here, the authors develop a single-cell small RNA sequencing method and report that a class of ~20-nt piRNAs lacking 3′ end 2′-O-methylation are associated with PIWIL3 protein and predominantly expressed in human...

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Autores principales: Qiyuan Yang, Ronghong Li, Qifeng Lyu, Li Hou, Zhen Liu, Qiang Sun, Miao Liu, Huijuan Shi, Beiying Xu, Mingru Yin, Zhiguang Yan, Ying Huang, Mofang Liu, Yiping Li, Ligang Wu
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/47021d58304e4f73bb96867ba072e9cd
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spelling oai:doaj.org-article:47021d58304e4f73bb96867ba072e9cd2021-12-02T15:36:05ZSingle-cell CAS-seq reveals a class of short PIWI-interacting RNAs in human oocytes10.1038/s41467-019-11312-82041-1723https://doaj.org/article/47021d58304e4f73bb96867ba072e9cd2019-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-11312-8https://doaj.org/toc/2041-1723PIWI-interacting RNAs (piRNAs) are ~25–33 nt small RNAs expressed in animal germ cells. Here, the authors develop a single-cell small RNA sequencing method and report that a class of ~20-nt piRNAs lacking 3′ end 2′-O-methylation are associated with PIWIL3 protein and predominantly expressed in human and monkey oocytes.Qiyuan YangRonghong LiQifeng LyuLi HouZhen LiuQiang SunMiao LiuHuijuan ShiBeiying XuMingru YinZhiguang YanYing HuangMofang LiuYiping LiLigang WuNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-15 (2019)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Qiyuan Yang
Ronghong Li
Qifeng Lyu
Li Hou
Zhen Liu
Qiang Sun
Miao Liu
Huijuan Shi
Beiying Xu
Mingru Yin
Zhiguang Yan
Ying Huang
Mofang Liu
Yiping Li
Ligang Wu
Single-cell CAS-seq reveals a class of short PIWI-interacting RNAs in human oocytes
description PIWI-interacting RNAs (piRNAs) are ~25–33 nt small RNAs expressed in animal germ cells. Here, the authors develop a single-cell small RNA sequencing method and report that a class of ~20-nt piRNAs lacking 3′ end 2′-O-methylation are associated with PIWIL3 protein and predominantly expressed in human and monkey oocytes.
format article
author Qiyuan Yang
Ronghong Li
Qifeng Lyu
Li Hou
Zhen Liu
Qiang Sun
Miao Liu
Huijuan Shi
Beiying Xu
Mingru Yin
Zhiguang Yan
Ying Huang
Mofang Liu
Yiping Li
Ligang Wu
author_facet Qiyuan Yang
Ronghong Li
Qifeng Lyu
Li Hou
Zhen Liu
Qiang Sun
Miao Liu
Huijuan Shi
Beiying Xu
Mingru Yin
Zhiguang Yan
Ying Huang
Mofang Liu
Yiping Li
Ligang Wu
author_sort Qiyuan Yang
title Single-cell CAS-seq reveals a class of short PIWI-interacting RNAs in human oocytes
title_short Single-cell CAS-seq reveals a class of short PIWI-interacting RNAs in human oocytes
title_full Single-cell CAS-seq reveals a class of short PIWI-interacting RNAs in human oocytes
title_fullStr Single-cell CAS-seq reveals a class of short PIWI-interacting RNAs in human oocytes
title_full_unstemmed Single-cell CAS-seq reveals a class of short PIWI-interacting RNAs in human oocytes
title_sort single-cell cas-seq reveals a class of short piwi-interacting rnas in human oocytes
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/47021d58304e4f73bb96867ba072e9cd
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