The marker of alkyl DNA base damage, N7-methylguanine, is associated with semen quality in men

Abstract Sperm DNA contains a range of DNA base damage that can arise, in part, from exposure to methylating agents. However, the effects are not fully characterized and so the aim of this study was to investigate associations between semen quality and the levels of N7-methyldeoxyguanosine (N7-MedG)...

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Autores principales:	B. Altakroni, C. Nevin, M. Carroll, C. Murgatroyd, G. Horne, D. R. Brison, A. C. Povey
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/47084c785ea64d00912fb185b08cab48
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spelling	oai:doaj.org-article:47084c785ea64d00912fb185b08cab482021-12-02T14:06:58ZThe marker of alkyl DNA base damage, N7-methylguanine, is associated with semen quality in men10.1038/s41598-021-81674-x2045-2322https://doaj.org/article/47084c785ea64d00912fb185b08cab482021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81674-xhttps://doaj.org/toc/2045-2322Abstract Sperm DNA contains a range of DNA base damage that can arise, in part, from exposure to methylating agents. However, the effects are not fully characterized and so the aim of this study was to investigate associations between semen quality and the levels of N7-methyldeoxyguanosine (N7-MedG), a marker of exposure to methylating agents, and other markers of DNA damage and DNA methylation. Sperm samples were collected from 105 men attending an assisted reproduction clinic as part of a couple undergoing treatment for infertility and semen quality assessed manually according to WHO guidelines. Semen levels of N7-MedG, quantified by immunoslotblot, were significantly higher in men with sperm concentration < 15 × 106/ml (p ≤ 0.01), semen volume < 1.5 ml (p ≤ 0.05) and also in men with any aspect of semen quality below WHO reference levels (p ≤ 0.001). Measures of neutral Comet DNA damage were correlated with semen quality in a univariate analysis but not after adjustment for N7-MedG levels. Sperm concentration was negatively associated with % methylation at the gene for DAZL but no other marker of global or gene-specific DNA methylation. Results support the hypothesis that the known toxic and DNA damaging properties of alkylating agent exposure may have direct deleterious consequences on semen quality.B. AltakroniC. NevinM. CarrollC. MurgatroydG. HorneD. R. BrisonA. C. PoveyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q B. Altakroni C. Nevin M. Carroll C. Murgatroyd G. Horne D. R. Brison A. C. Povey The marker of alkyl DNA base damage, N7-methylguanine, is associated with semen quality in men
description	Abstract Sperm DNA contains a range of DNA base damage that can arise, in part, from exposure to methylating agents. However, the effects are not fully characterized and so the aim of this study was to investigate associations between semen quality and the levels of N7-methyldeoxyguanosine (N7-MedG), a marker of exposure to methylating agents, and other markers of DNA damage and DNA methylation. Sperm samples were collected from 105 men attending an assisted reproduction clinic as part of a couple undergoing treatment for infertility and semen quality assessed manually according to WHO guidelines. Semen levels of N7-MedG, quantified by immunoslotblot, were significantly higher in men with sperm concentration < 15 × 106/ml (p ≤ 0.01), semen volume < 1.5 ml (p ≤ 0.05) and also in men with any aspect of semen quality below WHO reference levels (p ≤ 0.001). Measures of neutral Comet DNA damage were correlated with semen quality in a univariate analysis but not after adjustment for N7-MedG levels. Sperm concentration was negatively associated with % methylation at the gene for DAZL but no other marker of global or gene-specific DNA methylation. Results support the hypothesis that the known toxic and DNA damaging properties of alkylating agent exposure may have direct deleterious consequences on semen quality.
format	article
author	B. Altakroni C. Nevin M. Carroll C. Murgatroyd G. Horne D. R. Brison A. C. Povey
author_facet	B. Altakroni C. Nevin M. Carroll C. Murgatroyd G. Horne D. R. Brison A. C. Povey
author_sort	B. Altakroni
title	The marker of alkyl DNA base damage, N7-methylguanine, is associated with semen quality in men
title_short	The marker of alkyl DNA base damage, N7-methylguanine, is associated with semen quality in men
title_full	The marker of alkyl DNA base damage, N7-methylguanine, is associated with semen quality in men
title_fullStr	The marker of alkyl DNA base damage, N7-methylguanine, is associated with semen quality in men
title_full_unstemmed	The marker of alkyl DNA base damage, N7-methylguanine, is associated with semen quality in men
title_sort	marker of alkyl dna base damage, n7-methylguanine, is associated with semen quality in men
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/47084c785ea64d00912fb185b08cab48
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The marker of alkyl DNA base damage, N7-methylguanine, is associated with semen quality in men

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