Facile Synthesis of N-Doped Graphene Quantum Dots as Novel Transfection Agents for mRNA and pDNA

In the wake of the coronavirus disease 2019 (COVID-19) pandemic, global pharmaceutical companies have developed vaccines for the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Some have adopted lipid nanoparticles (LNPs) or viral vectors to deliver the genes associated with the spike...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Minchul Ahn, Jaekwang Song, Byung Hee Hong
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	gene delivery graphene quantum dots mRNA pDNA transfection Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/4709a826802e49099f9b0f3182126cf3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4709a826802e49099f9b0f3182126cf3
record_format	dspace
spelling	oai:doaj.org-article:4709a826802e49099f9b0f3182126cf32021-11-25T18:30:00ZFacile Synthesis of N-Doped Graphene Quantum Dots as Novel Transfection Agents for mRNA and pDNA10.3390/nano111128162079-4991https://doaj.org/article/4709a826802e49099f9b0f3182126cf32021-10-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/2816https://doaj.org/toc/2079-4991In the wake of the coronavirus disease 2019 (COVID-19) pandemic, global pharmaceutical companies have developed vaccines for the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Some have adopted lipid nanoparticles (LNPs) or viral vectors to deliver the genes associated with the spike protein of SARS-CoV-2 for vaccination. This strategy of vaccination by delivering genes to express viral proteins has been successfully applied to the mRNA vaccines for COVID-19, and is also applicable to gene therapy. However, conventional transfection agents such as LNPs and viral vectors are not yet sufficient to satisfy the levels of safety, stability, and efficiency required for the clinical applications of gene therapy. In this study, we synthesized N-doped graphene quantum dots (NGQDs) for the transfection of various genes, including messenger ribonucleic acids (mRNAs) and plasmid deoxyribonucleic acids (pDNAs). The positively charged NGQDs successfully formed electrostatic complexes with negatively charged mRNAs and pDNAs, and resulted in the efficient delivery and transfection of the genes into target cells. The transfection efficiency of NGQDs is found to be comparable to that of commercially available LNPs. Considering their outstanding stability even at room temperature as well as their low toxicity, NGQDs are expected to be novel universal gene delivery platforms that can outperform LNPs and viral vectors.Minchul AhnJaekwang SongByung Hee HongMDPI AGarticlegene deliverygraphene quantum dotsmRNApDNAtransfectionChemistryQD1-999ENNanomaterials, Vol 11, Iss 2816, p 2816 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gene delivery graphene quantum dots mRNA pDNA transfection Chemistry QD1-999
spellingShingle	gene delivery graphene quantum dots mRNA pDNA transfection Chemistry QD1-999 Minchul Ahn Jaekwang Song Byung Hee Hong Facile Synthesis of N-Doped Graphene Quantum Dots as Novel Transfection Agents for mRNA and pDNA
description	In the wake of the coronavirus disease 2019 (COVID-19) pandemic, global pharmaceutical companies have developed vaccines for the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Some have adopted lipid nanoparticles (LNPs) or viral vectors to deliver the genes associated with the spike protein of SARS-CoV-2 for vaccination. This strategy of vaccination by delivering genes to express viral proteins has been successfully applied to the mRNA vaccines for COVID-19, and is also applicable to gene therapy. However, conventional transfection agents such as LNPs and viral vectors are not yet sufficient to satisfy the levels of safety, stability, and efficiency required for the clinical applications of gene therapy. In this study, we synthesized N-doped graphene quantum dots (NGQDs) for the transfection of various genes, including messenger ribonucleic acids (mRNAs) and plasmid deoxyribonucleic acids (pDNAs). The positively charged NGQDs successfully formed electrostatic complexes with negatively charged mRNAs and pDNAs, and resulted in the efficient delivery and transfection of the genes into target cells. The transfection efficiency of NGQDs is found to be comparable to that of commercially available LNPs. Considering their outstanding stability even at room temperature as well as their low toxicity, NGQDs are expected to be novel universal gene delivery platforms that can outperform LNPs and viral vectors.
format	article
author	Minchul Ahn Jaekwang Song Byung Hee Hong
author_facet	Minchul Ahn Jaekwang Song Byung Hee Hong
author_sort	Minchul Ahn
title	Facile Synthesis of N-Doped Graphene Quantum Dots as Novel Transfection Agents for mRNA and pDNA
title_short	Facile Synthesis of N-Doped Graphene Quantum Dots as Novel Transfection Agents for mRNA and pDNA
title_full	Facile Synthesis of N-Doped Graphene Quantum Dots as Novel Transfection Agents for mRNA and pDNA
title_fullStr	Facile Synthesis of N-Doped Graphene Quantum Dots as Novel Transfection Agents for mRNA and pDNA
title_full_unstemmed	Facile Synthesis of N-Doped Graphene Quantum Dots as Novel Transfection Agents for mRNA and pDNA
title_sort	facile synthesis of n-doped graphene quantum dots as novel transfection agents for mrna and pdna
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4709a826802e49099f9b0f3182126cf3
work_keys_str_mv	AT minchulahn facilesynthesisofndopedgraphenequantumdotsasnoveltransfectionagentsformrnaandpdna AT jaekwangsong facilesynthesisofndopedgraphenequantumdotsasnoveltransfectionagentsformrnaandpdna AT byungheehong facilesynthesisofndopedgraphenequantumdotsasnoveltransfectionagentsformrnaandpdna
_version_	1718411071024267264

Facile Synthesis of N-Doped Graphene Quantum Dots as Novel Transfection Agents for mRNA and pDNA

Ejemplares similares