A quantitative analysis of 3D-cell distribution in regenerative muscle-skeletal system with synchrotron X-ray computed microtomography

Abstract One of the greatest enigmas of modern biology is how the geometry of muscular and skeletal structures are created and how their development is controlled during growth and regeneration. Scaling and shaping of vertebrate muscles and skeletal elements has always been enigmatic and required an...

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Autores principales:	Markéta Tesařová, Lucia Mancini, Andras Simon, Igor Adameyko, Markéta Kaucká, Ahmed Elewa, Gabriele Lanzafame, Yi Zhang, Dominika Kalasová, Bára Szarowská, Tomáš Zikmund, Marie Novotná, Jozef Kaiser
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Cartilaginous Elements Pleurodeles Waltl Muscle Attachment Points Inspection Technologies GmbH SYRMEP Beamline Medicine R Science Q
Acceso en línea:	https://doaj.org/article/470c1a0e0e47415aa4885626eb993803
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spelling	oai:doaj.org-article:470c1a0e0e47415aa4885626eb9938032021-12-02T15:08:16ZA quantitative analysis of 3D-cell distribution in regenerative muscle-skeletal system with synchrotron X-ray computed microtomography10.1038/s41598-018-32459-22045-2322https://doaj.org/article/470c1a0e0e47415aa4885626eb9938032018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32459-2https://doaj.org/toc/2045-2322Abstract One of the greatest enigmas of modern biology is how the geometry of muscular and skeletal structures are created and how their development is controlled during growth and regeneration. Scaling and shaping of vertebrate muscles and skeletal elements has always been enigmatic and required an advanced technical level in order to analyse the cell distribution in 3D. In this work, synchrotron X-ray computed microtomography (µCT) and chemical contrasting has been exploited for a quantitative analysis of the 3D-cell distribution in tissues of a developing salamander (Pleurodeles waltl) limb – a key model organism for vertebrate regeneration studies. We mapped the limb muscles, their size and shape as well as the number and density of cells within the extracellular matrix of the developing cartilage. By using tomographic approach, we explored the polarity of the cells in 3D, in relation to the structure of developing joints. We found that the polarity of chondrocytes correlates with the planes in joint surfaces and also changes along the length of the cartilaginous elements. Our approach generates data for the precise computer simulations of muscle-skeletal regeneration using cell dynamics models, which is necessary for the understanding how anisotropic growth results in the precise shapes of skeletal structures.Markéta TesařováLucia ManciniAndras SimonIgor AdameykoMarkéta KauckáAhmed ElewaGabriele LanzafameYi ZhangDominika KalasováBára SzarowskáTomáš ZikmundMarie NovotnáJozef KaiserNature PortfolioarticleCartilaginous ElementsPleurodeles WaltlMuscle Attachment PointsInspection Technologies GmbHSYRMEP BeamlineMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cartilaginous Elements Pleurodeles Waltl Muscle Attachment Points Inspection Technologies GmbH SYRMEP Beamline Medicine R Science Q
spellingShingle	Cartilaginous Elements Pleurodeles Waltl Muscle Attachment Points Inspection Technologies GmbH SYRMEP Beamline Medicine R Science Q Markéta Tesařová Lucia Mancini Andras Simon Igor Adameyko Markéta Kaucká Ahmed Elewa Gabriele Lanzafame Yi Zhang Dominika Kalasová Bára Szarowská Tomáš Zikmund Marie Novotná Jozef Kaiser A quantitative analysis of 3D-cell distribution in regenerative muscle-skeletal system with synchrotron X-ray computed microtomography
description	Abstract One of the greatest enigmas of modern biology is how the geometry of muscular and skeletal structures are created and how their development is controlled during growth and regeneration. Scaling and shaping of vertebrate muscles and skeletal elements has always been enigmatic and required an advanced technical level in order to analyse the cell distribution in 3D. In this work, synchrotron X-ray computed microtomography (µCT) and chemical contrasting has been exploited for a quantitative analysis of the 3D-cell distribution in tissues of a developing salamander (Pleurodeles waltl) limb – a key model organism for vertebrate regeneration studies. We mapped the limb muscles, their size and shape as well as the number and density of cells within the extracellular matrix of the developing cartilage. By using tomographic approach, we explored the polarity of the cells in 3D, in relation to the structure of developing joints. We found that the polarity of chondrocytes correlates with the planes in joint surfaces and also changes along the length of the cartilaginous elements. Our approach generates data for the precise computer simulations of muscle-skeletal regeneration using cell dynamics models, which is necessary for the understanding how anisotropic growth results in the precise shapes of skeletal structures.
format	article
author	Markéta Tesařová Lucia Mancini Andras Simon Igor Adameyko Markéta Kaucká Ahmed Elewa Gabriele Lanzafame Yi Zhang Dominika Kalasová Bára Szarowská Tomáš Zikmund Marie Novotná Jozef Kaiser
author_facet	Markéta Tesařová Lucia Mancini Andras Simon Igor Adameyko Markéta Kaucká Ahmed Elewa Gabriele Lanzafame Yi Zhang Dominika Kalasová Bára Szarowská Tomáš Zikmund Marie Novotná Jozef Kaiser
author_sort	Markéta Tesařová
title	A quantitative analysis of 3D-cell distribution in regenerative muscle-skeletal system with synchrotron X-ray computed microtomography
title_short	A quantitative analysis of 3D-cell distribution in regenerative muscle-skeletal system with synchrotron X-ray computed microtomography
title_full	A quantitative analysis of 3D-cell distribution in regenerative muscle-skeletal system with synchrotron X-ray computed microtomography
title_fullStr	A quantitative analysis of 3D-cell distribution in regenerative muscle-skeletal system with synchrotron X-ray computed microtomography
title_full_unstemmed	A quantitative analysis of 3D-cell distribution in regenerative muscle-skeletal system with synchrotron X-ray computed microtomography
title_sort	quantitative analysis of 3d-cell distribution in regenerative muscle-skeletal system with synchrotron x-ray computed microtomography
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/470c1a0e0e47415aa4885626eb993803
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A quantitative analysis of 3D-cell distribution in regenerative muscle-skeletal system with synchrotron X-ray computed microtomography

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