Isolation and characterization of Botryococcus braunii from a freshwater environment in Tenggarong, Kutai Kartanegara, Indonesia

Abstract. Nugroho RA, Subagyono DJN, Arung ET. 2020. Isolation and characterization of Botryococcus braunii from a freshwater environment in Tenggarong, Kutai Kartanegara, Indonesia. Biodiversitas 21: 2331-2336. The use of microalgae as an alternative source of oil has gained much attention in recen...

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Autores principales:	Rudy Agung Nugroho, RR Dirgarini Julia Nurlianti Subagyono, Enos Tangke Arung
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	botryococcus braunii, growth, hydrocarbon, lipid droplets Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/47125b789f9b4edf99451c84960e3e1a
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spelling	oai:doaj.org-article:47125b789f9b4edf99451c84960e3e1a2021-11-22T00:35:33ZIsolation and characterization of Botryococcus braunii from a freshwater environment in Tenggarong, Kutai Kartanegara, Indonesia1412-033X2085-472210.13057/biodiv/d210565https://doaj.org/article/47125b789f9b4edf99451c84960e3e1a2020-05-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5525https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Nugroho RA, Subagyono DJN, Arung ET. 2020. Isolation and characterization of Botryococcus braunii from a freshwater environment in Tenggarong, Kutai Kartanegara, Indonesia. Biodiversitas 21: 2331-2336. The use of microalgae as an alternative source of oil has gained much attention in recent years. The present study was conducted to isolate and characterize microalgae Botryococcus braunii from a freshwater environment, located in Tenggarong, Kutai Kartanegara, East Kalimantan, Indonesia. B. braunii were cultured and determined their growth in terms of biomass yield and the presence of lipid globules by red Nile staining. Dry biomass, phosphate, and protein content of cultured B. braunii were measured at 3 days of intervals for 24 days. Biomass yield, hydrocarbon, carotenoid, and chlorophyll content were determined at the pH range of 6, 6.5, 7, 7.5, and 8 and various CO2 concentrations. The result showed that B. braunii collected from freshwater in Tenggarong can produce lipid globules. The highest growth of B. braunii was reached at day 15, while the optimal phosphate content was on the 12th day, and the protein was relatively stable during culture. The optimal weight of B. braunii biomass was reached at pH 7 and 4% CO2 and the highest hydrocarbon content was obtained at pH 8 and 4% CO2. The highest chlorophyll and carotenoid contents were obtained 4% CO2. In conclusion, B. braunii collected from Tenggarong was successfully cultured and able to produce lipid globules. The optimal conditions for its growth and production of hydrocarbon, phosphate, protein, chlorophyll, and carotenoid were at pH of 7-8 and 4% CO2.Rudy Agung NugrohoRR Dirgarini Julia Nurlianti SubagyonoEnos Tangke ArungMBI & UNS Soloarticlebotryococcus braunii, growth, hydrocarbon, lipid dropletsBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 5 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	botryococcus braunii, growth, hydrocarbon, lipid droplets Biology (General) QH301-705.5
spellingShingle	botryococcus braunii, growth, hydrocarbon, lipid droplets Biology (General) QH301-705.5 Rudy Agung Nugroho RR Dirgarini Julia Nurlianti Subagyono Enos Tangke Arung Isolation and characterization of Botryococcus braunii from a freshwater environment in Tenggarong, Kutai Kartanegara, Indonesia
description	Abstract. Nugroho RA, Subagyono DJN, Arung ET. 2020. Isolation and characterization of Botryococcus braunii from a freshwater environment in Tenggarong, Kutai Kartanegara, Indonesia. Biodiversitas 21: 2331-2336. The use of microalgae as an alternative source of oil has gained much attention in recent years. The present study was conducted to isolate and characterize microalgae Botryococcus braunii from a freshwater environment, located in Tenggarong, Kutai Kartanegara, East Kalimantan, Indonesia. B. braunii were cultured and determined their growth in terms of biomass yield and the presence of lipid globules by red Nile staining. Dry biomass, phosphate, and protein content of cultured B. braunii were measured at 3 days of intervals for 24 days. Biomass yield, hydrocarbon, carotenoid, and chlorophyll content were determined at the pH range of 6, 6.5, 7, 7.5, and 8 and various CO2 concentrations. The result showed that B. braunii collected from freshwater in Tenggarong can produce lipid globules. The highest growth of B. braunii was reached at day 15, while the optimal phosphate content was on the 12th day, and the protein was relatively stable during culture. The optimal weight of B. braunii biomass was reached at pH 7 and 4% CO2 and the highest hydrocarbon content was obtained at pH 8 and 4% CO2. The highest chlorophyll and carotenoid contents were obtained 4% CO2. In conclusion, B. braunii collected from Tenggarong was successfully cultured and able to produce lipid globules. The optimal conditions for its growth and production of hydrocarbon, phosphate, protein, chlorophyll, and carotenoid were at pH of 7-8 and 4% CO2.
format	article
author	Rudy Agung Nugroho RR Dirgarini Julia Nurlianti Subagyono Enos Tangke Arung
author_facet	Rudy Agung Nugroho RR Dirgarini Julia Nurlianti Subagyono Enos Tangke Arung
author_sort	Rudy Agung Nugroho
title	Isolation and characterization of Botryococcus braunii from a freshwater environment in Tenggarong, Kutai Kartanegara, Indonesia
title_short	Isolation and characterization of Botryococcus braunii from a freshwater environment in Tenggarong, Kutai Kartanegara, Indonesia
title_full	Isolation and characterization of Botryococcus braunii from a freshwater environment in Tenggarong, Kutai Kartanegara, Indonesia
title_fullStr	Isolation and characterization of Botryococcus braunii from a freshwater environment in Tenggarong, Kutai Kartanegara, Indonesia
title_full_unstemmed	Isolation and characterization of Botryococcus braunii from a freshwater environment in Tenggarong, Kutai Kartanegara, Indonesia
title_sort	isolation and characterization of botryococcus braunii from a freshwater environment in tenggarong, kutai kartanegara, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/47125b789f9b4edf99451c84960e3e1a
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Isolation and characterization of Botryococcus braunii from a freshwater environment in Tenggarong, Kutai Kartanegara, Indonesia

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