Changes in Expression of Complement Components in the Ovine Spleen during Early Pregnancy

During early gestation in humans, complement regulation is essential for normal fetal growth. It is supposed that a complement pathway participates in maternal splenic immune regulation at the early stage of gestation in ewes. The aim of this study was to analyze the effects of early pregnancy on th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ling Yang, Luyu Wang, Jiaxuan Wu, Haichao Wang, Gengxin Yang, Leying Zhang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	pregnancy complement pathway immune regulation spleen sheep Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/47162d1f5f6c4d37b651d6d086601ca9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:47162d1f5f6c4d37b651d6d086601ca9
record_format	dspace
spelling	oai:doaj.org-article:47162d1f5f6c4d37b651d6d086601ca92021-11-25T16:18:32ZChanges in Expression of Complement Components in the Ovine Spleen during Early Pregnancy10.3390/ani111131832076-2615https://doaj.org/article/47162d1f5f6c4d37b651d6d086601ca92021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3183https://doaj.org/toc/2076-2615During early gestation in humans, complement regulation is essential for normal fetal growth. It is supposed that a complement pathway participates in maternal splenic immune regulation at the early stage of gestation in ewes. The aim of this study was to analyze the effects of early pregnancy on the expression of complement components in the maternal spleen of ewes. In this study, ovine spleens were sampled on day 16 of nonpregnancy, and days 13, 16 and 25 of gestation. RT-qPCR, Western blot and immunohistochemical analysis were used to detect the changes in expression of complement components in the ovine maternal spleens. Our results reveal that C1q was upregulated during early gestation, C1r, C1s, C2, C3 and C5b increased at day 25 of gestation and C4a and C9 peaked at days 13 and 16 of gestation. In addition, C3 protein was located in the capsule, trabeculae and splenic cords. In conclusion, our results show for the first time that there was modification in the expression of complement components in the ovine spleen at the early stage of gestation, and complement pathways may participate in modulating splenic immune responses at the early stage of gestation.Ling YangLuyu WangJiaxuan WuHaichao WangGengxin YangLeying ZhangMDPI AGarticlepregnancycomplement pathwayimmune regulationspleensheepVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3183, p 3183 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pregnancy complement pathway immune regulation spleen sheep Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	pregnancy complement pathway immune regulation spleen sheep Veterinary medicine SF600-1100 Zoology QL1-991 Ling Yang Luyu Wang Jiaxuan Wu Haichao Wang Gengxin Yang Leying Zhang Changes in Expression of Complement Components in the Ovine Spleen during Early Pregnancy
description	During early gestation in humans, complement regulation is essential for normal fetal growth. It is supposed that a complement pathway participates in maternal splenic immune regulation at the early stage of gestation in ewes. The aim of this study was to analyze the effects of early pregnancy on the expression of complement components in the maternal spleen of ewes. In this study, ovine spleens were sampled on day 16 of nonpregnancy, and days 13, 16 and 25 of gestation. RT-qPCR, Western blot and immunohistochemical analysis were used to detect the changes in expression of complement components in the ovine maternal spleens. Our results reveal that C1q was upregulated during early gestation, C1r, C1s, C2, C3 and C5b increased at day 25 of gestation and C4a and C9 peaked at days 13 and 16 of gestation. In addition, C3 protein was located in the capsule, trabeculae and splenic cords. In conclusion, our results show for the first time that there was modification in the expression of complement components in the ovine spleen at the early stage of gestation, and complement pathways may participate in modulating splenic immune responses at the early stage of gestation.
format	article
author	Ling Yang Luyu Wang Jiaxuan Wu Haichao Wang Gengxin Yang Leying Zhang
author_facet	Ling Yang Luyu Wang Jiaxuan Wu Haichao Wang Gengxin Yang Leying Zhang
author_sort	Ling Yang
title	Changes in Expression of Complement Components in the Ovine Spleen during Early Pregnancy
title_short	Changes in Expression of Complement Components in the Ovine Spleen during Early Pregnancy
title_full	Changes in Expression of Complement Components in the Ovine Spleen during Early Pregnancy
title_fullStr	Changes in Expression of Complement Components in the Ovine Spleen during Early Pregnancy
title_full_unstemmed	Changes in Expression of Complement Components in the Ovine Spleen during Early Pregnancy
title_sort	changes in expression of complement components in the ovine spleen during early pregnancy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/47162d1f5f6c4d37b651d6d086601ca9
work_keys_str_mv	AT lingyang changesinexpressionofcomplementcomponentsintheovinespleenduringearlypregnancy AT luyuwang changesinexpressionofcomplementcomponentsintheovinespleenduringearlypregnancy AT jiaxuanwu changesinexpressionofcomplementcomponentsintheovinespleenduringearlypregnancy AT haichaowang changesinexpressionofcomplementcomponentsintheovinespleenduringearlypregnancy AT gengxinyang changesinexpressionofcomplementcomponentsintheovinespleenduringearlypregnancy AT leyingzhang changesinexpressionofcomplementcomponentsintheovinespleenduringearlypregnancy
_version_	1718413253591171072

Changes in Expression of Complement Components in the Ovine Spleen during Early Pregnancy

Ejemplares similares