Genome-wide screen of genes required for caffeine tolerance in fission yeast.

<h4>Background</h4>An excess of caffeine is cytotoxic to all eukaryotic cell types. We aim to study how cells become tolerant to a toxic dose of this drug, and the relationship between caffeine and oxidative stress pathways.<h4>Methodology/principal findings</h4>We searched f...

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Autores principales:	Isabel A Calvo, Natalia Gabrielli, Iván Iglesias-Baena, Sarela García-Santamarina, Kwang-Lae Hoe, Dong Uk Kim, Miriam Sansó, Alice Zuin, Pilar Pérez, José Ayté, Elena Hidalgo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/471ade7ebfe54409a0bfa95fab07d3f7
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spelling	oai:doaj.org-article:471ade7ebfe54409a0bfa95fab07d3f72021-11-25T06:21:03ZGenome-wide screen of genes required for caffeine tolerance in fission yeast.1932-620310.1371/journal.pone.0006619https://doaj.org/article/471ade7ebfe54409a0bfa95fab07d3f72009-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19672306/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>An excess of caffeine is cytotoxic to all eukaryotic cell types. We aim to study how cells become tolerant to a toxic dose of this drug, and the relationship between caffeine and oxidative stress pathways.<h4>Methodology/principal findings</h4>We searched for Schizosaccharomyces pombe mutants with inhibited growth on caffeine-containing plates. We screened a collection of 2,700 haploid mutant cells, of which 98 were sensitive to caffeine. The genes mutated in these sensitive clones were involved in a number of cellular roles including the H(2)O(2)-induced Pap1 and Sty1 stress pathways, the integrity and calcineurin pathways, cell morphology and chromatin remodeling. We have investigated the role of the oxidative stress pathways in sensing and promoting survival to caffeine. The Pap1 and the Sty1 pathways are both required for normal tolerance to caffeine, but only the Sty1 pathway is activated by the drug. Cells lacking Pap1 are sensitive to caffeine due to the decreased expression of the efflux pump Hba2. Indeed, ?hba2 cells are sensitive to caffeine, and constitutive activation of the Pap1 pathway enhances resistance to caffeine in an Hba2-dependent manner.<h4>Conclusions/significance</h4>With our caffeine-sensitive, genome-wide screen of an S. pombe deletion collection, we have demonstrated the importance of some oxidative stress pathway components on wild-type tolerance to the drug.Isabel A CalvoNatalia GabrielliIván Iglesias-BaenaSarela García-SantamarinaKwang-Lae HoeDong Uk KimMiriam SansóAlice ZuinPilar PérezJosé AytéElena HidalgoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 8, p e6619 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Isabel A Calvo Natalia Gabrielli Iván Iglesias-Baena Sarela García-Santamarina Kwang-Lae Hoe Dong Uk Kim Miriam Sansó Alice Zuin Pilar Pérez José Ayté Elena Hidalgo Genome-wide screen of genes required for caffeine tolerance in fission yeast.
description	<h4>Background</h4>An excess of caffeine is cytotoxic to all eukaryotic cell types. We aim to study how cells become tolerant to a toxic dose of this drug, and the relationship between caffeine and oxidative stress pathways.<h4>Methodology/principal findings</h4>We searched for Schizosaccharomyces pombe mutants with inhibited growth on caffeine-containing plates. We screened a collection of 2,700 haploid mutant cells, of which 98 were sensitive to caffeine. The genes mutated in these sensitive clones were involved in a number of cellular roles including the H(2)O(2)-induced Pap1 and Sty1 stress pathways, the integrity and calcineurin pathways, cell morphology and chromatin remodeling. We have investigated the role of the oxidative stress pathways in sensing and promoting survival to caffeine. The Pap1 and the Sty1 pathways are both required for normal tolerance to caffeine, but only the Sty1 pathway is activated by the drug. Cells lacking Pap1 are sensitive to caffeine due to the decreased expression of the efflux pump Hba2. Indeed, ?hba2 cells are sensitive to caffeine, and constitutive activation of the Pap1 pathway enhances resistance to caffeine in an Hba2-dependent manner.<h4>Conclusions/significance</h4>With our caffeine-sensitive, genome-wide screen of an S. pombe deletion collection, we have demonstrated the importance of some oxidative stress pathway components on wild-type tolerance to the drug.
format	article
author	Isabel A Calvo Natalia Gabrielli Iván Iglesias-Baena Sarela García-Santamarina Kwang-Lae Hoe Dong Uk Kim Miriam Sansó Alice Zuin Pilar Pérez José Ayté Elena Hidalgo
author_facet	Isabel A Calvo Natalia Gabrielli Iván Iglesias-Baena Sarela García-Santamarina Kwang-Lae Hoe Dong Uk Kim Miriam Sansó Alice Zuin Pilar Pérez José Ayté Elena Hidalgo
author_sort	Isabel A Calvo
title	Genome-wide screen of genes required for caffeine tolerance in fission yeast.
title_short	Genome-wide screen of genes required for caffeine tolerance in fission yeast.
title_full	Genome-wide screen of genes required for caffeine tolerance in fission yeast.
title_fullStr	Genome-wide screen of genes required for caffeine tolerance in fission yeast.
title_full_unstemmed	Genome-wide screen of genes required for caffeine tolerance in fission yeast.
title_sort	genome-wide screen of genes required for caffeine tolerance in fission yeast.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/471ade7ebfe54409a0bfa95fab07d3f7
work_keys_str_mv	AT isabelacalvo genomewidescreenofgenesrequiredforcaffeinetoleranceinfissionyeast AT nataliagabrielli genomewidescreenofgenesrequiredforcaffeinetoleranceinfissionyeast AT ivaniglesiasbaena genomewidescreenofgenesrequiredforcaffeinetoleranceinfissionyeast AT sarelagarciasantamarina genomewidescreenofgenesrequiredforcaffeinetoleranceinfissionyeast AT kwanglaehoe genomewidescreenofgenesrequiredforcaffeinetoleranceinfissionyeast AT dongukkim genomewidescreenofgenesrequiredforcaffeinetoleranceinfissionyeast AT miriamsanso genomewidescreenofgenesrequiredforcaffeinetoleranceinfissionyeast AT alicezuin genomewidescreenofgenesrequiredforcaffeinetoleranceinfissionyeast AT pilarperez genomewidescreenofgenesrequiredforcaffeinetoleranceinfissionyeast AT joseayte genomewidescreenofgenesrequiredforcaffeinetoleranceinfissionyeast AT elenahidalgo genomewidescreenofgenesrequiredforcaffeinetoleranceinfissionyeast
_version_	1718413799042580480

Genome-wide screen of genes required for caffeine tolerance in fission yeast.

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