Placental O-GlcNAc-transferase expression and interactions with the glucocorticoid receptor are sex specific and regulated by maternal corticosterone exposure in mice

Abstract Maternal stress programs offspring disease in a sexually dimorphic manner with males often more adversely affected. Previous studies of maternal glucocorticoid exposure suggest male vulnerability may derive from placental alterations. The hexosamine signalling pathway and O-linked glycosyla...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Marie Pantaleon, Sarah E. Steane, Kathryn McMahon, James S. M. Cuffe, Karen M. Moritz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/47200b0bb25a44ee8394b538d7895a3c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:47200b0bb25a44ee8394b538d7895a3c
record_format	dspace
spelling	oai:doaj.org-article:47200b0bb25a44ee8394b538d7895a3c2021-12-02T15:05:48ZPlacental O-GlcNAc-transferase expression and interactions with the glucocorticoid receptor are sex specific and regulated by maternal corticosterone exposure in mice10.1038/s41598-017-01666-82045-2322https://doaj.org/article/47200b0bb25a44ee8394b538d7895a3c2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01666-8https://doaj.org/toc/2045-2322Abstract Maternal stress programs offspring disease in a sexually dimorphic manner with males often more adversely affected. Previous studies of maternal glucocorticoid exposure suggest male vulnerability may derive from placental alterations. The hexosamine signalling pathway and O-linked glycosylation (O-GlcNAcylation) are part of an essential adaptive survival response in healthy cells. The key enzyme involved is O-linked-N-acetylglucosamine transferase (OGT), a gene recently identified as a sex-specific placental biomarker of maternal stress. Using a mouse model of maternal corticosterone (Cort) exposure, we examined components of hexosamine biosynthesis/signalling and O-GlcNAcylation in whole placentae at E14.5. Our results demonstrate sex-specific differences in OGT levels and O-GlcNAcylation during Cort exposure which impacts on key mediators of cell survival, in particular AKT as well as the stress responsive OGT/GR transrepression complex. In male placentae only, Cort exposure increased Akt O-GlcNacylation which correlated with decreased phosphorylation. Female placentae had higher basal OGT and OGT/GR complex compared with male placentae. Cort exposure did not alter these levels in female placentae but increased global O-GlcNacylation. In male placentae Cort increased OGT and OGT/GR complex with no change in global O-GlcNacylation. These findings suggest that sex-specific differences in placental OGT play a key role in the sexually dimorphic responses to stress.Marie PantaleonSarah E. SteaneKathryn McMahonJames S. M. CuffeKaren M. MoritzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marie Pantaleon Sarah E. Steane Kathryn McMahon James S. M. Cuffe Karen M. Moritz Placental O-GlcNAc-transferase expression and interactions with the glucocorticoid receptor are sex specific and regulated by maternal corticosterone exposure in mice
description	Abstract Maternal stress programs offspring disease in a sexually dimorphic manner with males often more adversely affected. Previous studies of maternal glucocorticoid exposure suggest male vulnerability may derive from placental alterations. The hexosamine signalling pathway and O-linked glycosylation (O-GlcNAcylation) are part of an essential adaptive survival response in healthy cells. The key enzyme involved is O-linked-N-acetylglucosamine transferase (OGT), a gene recently identified as a sex-specific placental biomarker of maternal stress. Using a mouse model of maternal corticosterone (Cort) exposure, we examined components of hexosamine biosynthesis/signalling and O-GlcNAcylation in whole placentae at E14.5. Our results demonstrate sex-specific differences in OGT levels and O-GlcNAcylation during Cort exposure which impacts on key mediators of cell survival, in particular AKT as well as the stress responsive OGT/GR transrepression complex. In male placentae only, Cort exposure increased Akt O-GlcNacylation which correlated with decreased phosphorylation. Female placentae had higher basal OGT and OGT/GR complex compared with male placentae. Cort exposure did not alter these levels in female placentae but increased global O-GlcNacylation. In male placentae Cort increased OGT and OGT/GR complex with no change in global O-GlcNacylation. These findings suggest that sex-specific differences in placental OGT play a key role in the sexually dimorphic responses to stress.
format	article
author	Marie Pantaleon Sarah E. Steane Kathryn McMahon James S. M. Cuffe Karen M. Moritz
author_facet	Marie Pantaleon Sarah E. Steane Kathryn McMahon James S. M. Cuffe Karen M. Moritz
author_sort	Marie Pantaleon
title	Placental O-GlcNAc-transferase expression and interactions with the glucocorticoid receptor are sex specific and regulated by maternal corticosterone exposure in mice
title_short	Placental O-GlcNAc-transferase expression and interactions with the glucocorticoid receptor are sex specific and regulated by maternal corticosterone exposure in mice
title_full	Placental O-GlcNAc-transferase expression and interactions with the glucocorticoid receptor are sex specific and regulated by maternal corticosterone exposure in mice
title_fullStr	Placental O-GlcNAc-transferase expression and interactions with the glucocorticoid receptor are sex specific and regulated by maternal corticosterone exposure in mice
title_full_unstemmed	Placental O-GlcNAc-transferase expression and interactions with the glucocorticoid receptor are sex specific and regulated by maternal corticosterone exposure in mice
title_sort	placental o-glcnac-transferase expression and interactions with the glucocorticoid receptor are sex specific and regulated by maternal corticosterone exposure in mice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/47200b0bb25a44ee8394b538d7895a3c
work_keys_str_mv	AT mariepantaleon placentaloglcnactransferaseexpressionandinteractionswiththeglucocorticoidreceptoraresexspecificandregulatedbymaternalcorticosteroneexposureinmice AT sarahesteane placentaloglcnactransferaseexpressionandinteractionswiththeglucocorticoidreceptoraresexspecificandregulatedbymaternalcorticosteroneexposureinmice AT kathrynmcmahon placentaloglcnactransferaseexpressionandinteractionswiththeglucocorticoidreceptoraresexspecificandregulatedbymaternalcorticosteroneexposureinmice AT jamessmcuffe placentaloglcnactransferaseexpressionandinteractionswiththeglucocorticoidreceptoraresexspecificandregulatedbymaternalcorticosteroneexposureinmice AT karenmmoritz placentaloglcnactransferaseexpressionandinteractionswiththeglucocorticoidreceptoraresexspecificandregulatedbymaternalcorticosteroneexposureinmice
_version_	1718388701006921728

Placental O-GlcNAc-transferase expression and interactions with the glucocorticoid receptor are sex specific and regulated by maternal corticosterone exposure in mice

Ejemplares similares