Role of MS1 homolog Ntms1 gene of tobacco infertility

The sterile line is the basis of crop heterosis utilization. To broaden the sources of male sterility in tobacco, the Ntms1 (Nicotiana tabacum L. ms1) gene was cloned from the tobacco variety K326 by homologous cloning based on the Cams1 (Capsicum annuum L. ms1) gene sequence of male-sterility genes...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chen Qian, Zhao Tingting, Duan Lili, Mo Zejun, Tian Maozhu, Li Zhenhua, Liu Yang, Liu Renxiang
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	ms1 gene tobacco fertility homologous cloning Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4723a0c049bf44ceaba6ca33e151975a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4723a0c049bf44ceaba6ca33e151975a
record_format	dspace
spelling	oai:doaj.org-article:4723a0c049bf44ceaba6ca33e151975a2021-12-05T14:10:41ZRole of MS1 homolog Ntms1 gene of tobacco infertility2391-541210.1515/biol-2021-0087https://doaj.org/article/4723a0c049bf44ceaba6ca33e151975a2021-08-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0087https://doaj.org/toc/2391-5412The sterile line is the basis of crop heterosis utilization. To broaden the sources of male sterility in tobacco, the Ntms1 (Nicotiana tabacum L. ms1) gene was cloned from the tobacco variety K326 by homologous cloning based on the Cams1 (Capsicum annuum L. ms1) gene sequence of male-sterility genes in pepper. The protein structure and physicochemical properties of the two genes were determined by bioinformatics analysis, and the function of the Ntms1 gene was verified by the CRISPR/Cas9 system. The results showed that the sequences of Ntms1 and Cams1 were 85.25% similar, and plant homeodomains were found in both genes; the physical and chemical properties were also very similar. It is speculated that the Ntms1 gene had the same function as the Cams1 gene in controlling male sterility. Compared to the wild-type plants, the filaments of the Ntms1 knockout mutant plants were shorter, and the stamen was shorter than the pistil. The anthers did not develop fully and had few viable pollen grains; the tapetum and the anther wall had developed abnormally, and the anther chamber was severely squeezed. The malondialdehyde content in the mutant plants was significantly higher than that in the wild-type plants, while self-fertility was significantly lower in the mutant plants. The results showed that the Ntms1 gene plays an important role in regulating fertility in tobacco.Chen QianZhao TingtingDuan LiliMo ZejunTian MaozhuLi ZhenhuaLiu YangLiu RenxiangDe Gruyterarticlems1 genetobaccofertilityhomologous cloningBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 815-826 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ms1 gene tobacco fertility homologous cloning Biology (General) QH301-705.5
spellingShingle	ms1 gene tobacco fertility homologous cloning Biology (General) QH301-705.5 Chen Qian Zhao Tingting Duan Lili Mo Zejun Tian Maozhu Li Zhenhua Liu Yang Liu Renxiang Role of MS1 homolog Ntms1 gene of tobacco infertility
description	The sterile line is the basis of crop heterosis utilization. To broaden the sources of male sterility in tobacco, the Ntms1 (Nicotiana tabacum L. ms1) gene was cloned from the tobacco variety K326 by homologous cloning based on the Cams1 (Capsicum annuum L. ms1) gene sequence of male-sterility genes in pepper. The protein structure and physicochemical properties of the two genes were determined by bioinformatics analysis, and the function of the Ntms1 gene was verified by the CRISPR/Cas9 system. The results showed that the sequences of Ntms1 and Cams1 were 85.25% similar, and plant homeodomains were found in both genes; the physical and chemical properties were also very similar. It is speculated that the Ntms1 gene had the same function as the Cams1 gene in controlling male sterility. Compared to the wild-type plants, the filaments of the Ntms1 knockout mutant plants were shorter, and the stamen was shorter than the pistil. The anthers did not develop fully and had few viable pollen grains; the tapetum and the anther wall had developed abnormally, and the anther chamber was severely squeezed. The malondialdehyde content in the mutant plants was significantly higher than that in the wild-type plants, while self-fertility was significantly lower in the mutant plants. The results showed that the Ntms1 gene plays an important role in regulating fertility in tobacco.
format	article
author	Chen Qian Zhao Tingting Duan Lili Mo Zejun Tian Maozhu Li Zhenhua Liu Yang Liu Renxiang
author_facet	Chen Qian Zhao Tingting Duan Lili Mo Zejun Tian Maozhu Li Zhenhua Liu Yang Liu Renxiang
author_sort	Chen Qian
title	Role of MS1 homolog Ntms1 gene of tobacco infertility
title_short	Role of MS1 homolog Ntms1 gene of tobacco infertility
title_full	Role of MS1 homolog Ntms1 gene of tobacco infertility
title_fullStr	Role of MS1 homolog Ntms1 gene of tobacco infertility
title_full_unstemmed	Role of MS1 homolog Ntms1 gene of tobacco infertility
title_sort	role of ms1 homolog ntms1 gene of tobacco infertility
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/4723a0c049bf44ceaba6ca33e151975a
work_keys_str_mv	AT chenqian roleofms1homologntms1geneoftobaccoinfertility AT zhaotingting roleofms1homologntms1geneoftobaccoinfertility AT duanlili roleofms1homologntms1geneoftobaccoinfertility AT mozejun roleofms1homologntms1geneoftobaccoinfertility AT tianmaozhu roleofms1homologntms1geneoftobaccoinfertility AT lizhenhua roleofms1homologntms1geneoftobaccoinfertility AT liuyang roleofms1homologntms1geneoftobaccoinfertility AT liurenxiang roleofms1homologntms1geneoftobaccoinfertility
_version_	1718371837376724992

Role of MS1 homolog Ntms1 gene of tobacco infertility

Ejemplares similares