The viral distribution and pathological characteristics of BALB/c mice infected with highly pathogenic Influenza H7N9 virus

Abstract Background The highly pathogenic Influenza H7N9 virus is believed to cause multiple organ infections. However, there have been few systematic animal experiments demonstrating the virus distribution after H7N9 virus infection. The present study was carried out to investigate the viral distri...

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Autores principales:	Xiao-Xin Wu, Song-Jia Tang, Shu-Hao Yao, Yu-Qin Zhou, Lan-Lan Xiao, Lin-Fang Cheng, Fu-Ming Liu, Nan-Ping Wu, Hang-Ping Yao, Lan-Juan Li
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Highly pathogenic H7N9 Multiple organ injury Viral distribution Pathological changes Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/472568dc9858496eaae28b15aa8c1c03
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spelling	oai:doaj.org-article:472568dc9858496eaae28b15aa8c1c032021-12-05T12:03:06ZThe viral distribution and pathological characteristics of BALB/c mice infected with highly pathogenic Influenza H7N9 virus10.1186/s12985-021-01709-71743-422Xhttps://doaj.org/article/472568dc9858496eaae28b15aa8c1c032021-11-01T00:00:00Zhttps://doi.org/10.1186/s12985-021-01709-7https://doaj.org/toc/1743-422XAbstract Background The highly pathogenic Influenza H7N9 virus is believed to cause multiple organ infections. However, there have been few systematic animal experiments demonstrating the virus distribution after H7N9 virus infection. The present study was carried out to investigate the viral distribution and pathological changes in the main organs of mice after experimental infection with highly pathogenic H7N9 virus. Methods Infection of mice with A/Guangdong/GZ8H002/2017(H7N9) virus was achieved via nasal inoculation. Mice were killed at 2, 3, and 7 days post infection. The other mice were used to observe their illness status and weight changes. Reverse transcription polymerase chain reaction and viral isolation were used to analyse the characteristics of viral invasion. The pathological changes of the main organs were observed using haematoxylin and eosin staining and immunohistochemistry. Results The weight of H7N9 virus-infected mice increased slightly in the first two days. However, the weight of the mice decreased sharply in the following days, by up to 20%. All the mice had died by the 8th day post infection and showed multiple organ injury. The emergence of viremia in mice was synchronous with lung infection. On the third day post infection, except in the brain, the virus could be isolated from all organs (lung, heart, kidney, liver, and spleen). On the seventh day post infection, the virus could be detected in all six organs. Brain infection was detected in all mice, and the viral titre in the heart, kidney, and spleen infection was high. Conclusion Acute diffuse lung injury was the initial pathogenesis in highly pathogenic H7N9 virus infection. In addition to lung infection and viremia, the highly pathogenic H7N9 virus could cause multiple organ infection and injury.Xiao-Xin WuSong-Jia TangShu-Hao YaoYu-Qin ZhouLan-Lan XiaoLin-Fang ChengFu-Ming LiuNan-Ping WuHang-Ping YaoLan-Juan LiBMCarticleHighly pathogenic H7N9Multiple organ injuryViral distributionPathological changesInfectious and parasitic diseasesRC109-216ENVirology Journal, Vol 18, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Highly pathogenic H7N9 Multiple organ injury Viral distribution Pathological changes Infectious and parasitic diseases RC109-216
spellingShingle	Highly pathogenic H7N9 Multiple organ injury Viral distribution Pathological changes Infectious and parasitic diseases RC109-216 Xiao-Xin Wu Song-Jia Tang Shu-Hao Yao Yu-Qin Zhou Lan-Lan Xiao Lin-Fang Cheng Fu-Ming Liu Nan-Ping Wu Hang-Ping Yao Lan-Juan Li The viral distribution and pathological characteristics of BALB/c mice infected with highly pathogenic Influenza H7N9 virus
description	Abstract Background The highly pathogenic Influenza H7N9 virus is believed to cause multiple organ infections. However, there have been few systematic animal experiments demonstrating the virus distribution after H7N9 virus infection. The present study was carried out to investigate the viral distribution and pathological changes in the main organs of mice after experimental infection with highly pathogenic H7N9 virus. Methods Infection of mice with A/Guangdong/GZ8H002/2017(H7N9) virus was achieved via nasal inoculation. Mice were killed at 2, 3, and 7 days post infection. The other mice were used to observe their illness status and weight changes. Reverse transcription polymerase chain reaction and viral isolation were used to analyse the characteristics of viral invasion. The pathological changes of the main organs were observed using haematoxylin and eosin staining and immunohistochemistry. Results The weight of H7N9 virus-infected mice increased slightly in the first two days. However, the weight of the mice decreased sharply in the following days, by up to 20%. All the mice had died by the 8th day post infection and showed multiple organ injury. The emergence of viremia in mice was synchronous with lung infection. On the third day post infection, except in the brain, the virus could be isolated from all organs (lung, heart, kidney, liver, and spleen). On the seventh day post infection, the virus could be detected in all six organs. Brain infection was detected in all mice, and the viral titre in the heart, kidney, and spleen infection was high. Conclusion Acute diffuse lung injury was the initial pathogenesis in highly pathogenic H7N9 virus infection. In addition to lung infection and viremia, the highly pathogenic H7N9 virus could cause multiple organ infection and injury.
format	article
author	Xiao-Xin Wu Song-Jia Tang Shu-Hao Yao Yu-Qin Zhou Lan-Lan Xiao Lin-Fang Cheng Fu-Ming Liu Nan-Ping Wu Hang-Ping Yao Lan-Juan Li
author_facet	Xiao-Xin Wu Song-Jia Tang Shu-Hao Yao Yu-Qin Zhou Lan-Lan Xiao Lin-Fang Cheng Fu-Ming Liu Nan-Ping Wu Hang-Ping Yao Lan-Juan Li
author_sort	Xiao-Xin Wu
title	The viral distribution and pathological characteristics of BALB/c mice infected with highly pathogenic Influenza H7N9 virus
title_short	The viral distribution and pathological characteristics of BALB/c mice infected with highly pathogenic Influenza H7N9 virus
title_full	The viral distribution and pathological characteristics of BALB/c mice infected with highly pathogenic Influenza H7N9 virus
title_fullStr	The viral distribution and pathological characteristics of BALB/c mice infected with highly pathogenic Influenza H7N9 virus
title_full_unstemmed	The viral distribution and pathological characteristics of BALB/c mice infected with highly pathogenic Influenza H7N9 virus
title_sort	viral distribution and pathological characteristics of balb/c mice infected with highly pathogenic influenza h7n9 virus
publisher	BMC
publishDate	2021
url	https://doaj.org/article/472568dc9858496eaae28b15aa8c1c03
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The viral distribution and pathological characteristics of BALB/c mice infected with highly pathogenic Influenza H7N9 virus

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