N6-Methyladenosine-regulated LINC00675 Suppress the Proliferation, Migration and Invasion of Breast Cancer cells via Inhibiting miR-513b-5p

Breast cancer (BC) is the most common cancer among women. LINC00675 and miR-513b-5p has been reported to be abnormally expressed in multiple types of cancers and modulate malignant phenotypes of cancer cells. However, to date, the functional role and underlying regulatory mechanism of LINC00675 and...

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Autores principales:	Shenglan Fan, Liping Wang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	mir-513b-5p cerna m6a methylation mettl3 Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/47285d3a21764eb58817f58a18ea754b
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spelling	oai:doaj.org-article:47285d3a21764eb58817f58a18ea754b2021-11-11T14:23:43ZN6-Methyladenosine-regulated LINC00675 Suppress the Proliferation, Migration and Invasion of Breast Cancer cells via Inhibiting miR-513b-5p2165-59792165-598710.1080/21655979.2021.2001905https://doaj.org/article/47285d3a21764eb58817f58a18ea754b2021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2001905https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Breast cancer (BC) is the most common cancer among women. LINC00675 and miR-513b-5p has been reported to be abnormally expressed in multiple types of cancers and modulate malignant phenotypes of cancer cells. However, to date, the functional role and underlying regulatory mechanism of LINC00675 and miR-513b-5p in BC remains largely unknown. Here, we found that LINC00675 was significantly downregulated in BC tissues and cell lines. Decrease of LINC00675 expression associated with higher tumor grade, lymphovascular invasion and shorter survival in BC patients. Functional experiments demonstrated that overexpression of LINC00675 suppressed BC cell proliferation, migration and invasion, whereas depletion of LINC00675 exerted opposite effects. Mechanistically, LINC00675 functioned as a competing endogenous RNA (ceRNA) to interact with miR-513b-5p and suppress its expression. Moreover, METTL3 increased the m6A methylation of LINC00675, which enhanced the association between LINC00675 and miR-513b-5p. Collectively, the central findings of our study suggest that LINC00675 represses BC progression through the inhibition of miR-513b-5p in a m6A-dependent manner.Shenglan FanLiping WangTaylor & Francis Grouparticlemir-513b-5pcernam6a methylationmettl3BiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mir-513b-5p cerna m6a methylation mettl3 Biotechnology TP248.13-248.65
spellingShingle	mir-513b-5p cerna m6a methylation mettl3 Biotechnology TP248.13-248.65 Shenglan Fan Liping Wang N6-Methyladenosine-regulated LINC00675 Suppress the Proliferation, Migration and Invasion of Breast Cancer cells via Inhibiting miR-513b-5p
description	Breast cancer (BC) is the most common cancer among women. LINC00675 and miR-513b-5p has been reported to be abnormally expressed in multiple types of cancers and modulate malignant phenotypes of cancer cells. However, to date, the functional role and underlying regulatory mechanism of LINC00675 and miR-513b-5p in BC remains largely unknown. Here, we found that LINC00675 was significantly downregulated in BC tissues and cell lines. Decrease of LINC00675 expression associated with higher tumor grade, lymphovascular invasion and shorter survival in BC patients. Functional experiments demonstrated that overexpression of LINC00675 suppressed BC cell proliferation, migration and invasion, whereas depletion of LINC00675 exerted opposite effects. Mechanistically, LINC00675 functioned as a competing endogenous RNA (ceRNA) to interact with miR-513b-5p and suppress its expression. Moreover, METTL3 increased the m6A methylation of LINC00675, which enhanced the association between LINC00675 and miR-513b-5p. Collectively, the central findings of our study suggest that LINC00675 represses BC progression through the inhibition of miR-513b-5p in a m6A-dependent manner.
format	article
author	Shenglan Fan Liping Wang
author_facet	Shenglan Fan Liping Wang
author_sort	Shenglan Fan
title	N6-Methyladenosine-regulated LINC00675 Suppress the Proliferation, Migration and Invasion of Breast Cancer cells via Inhibiting miR-513b-5p
title_short	N6-Methyladenosine-regulated LINC00675 Suppress the Proliferation, Migration and Invasion of Breast Cancer cells via Inhibiting miR-513b-5p
title_full	N6-Methyladenosine-regulated LINC00675 Suppress the Proliferation, Migration and Invasion of Breast Cancer cells via Inhibiting miR-513b-5p
title_fullStr	N6-Methyladenosine-regulated LINC00675 Suppress the Proliferation, Migration and Invasion of Breast Cancer cells via Inhibiting miR-513b-5p
title_full_unstemmed	N6-Methyladenosine-regulated LINC00675 Suppress the Proliferation, Migration and Invasion of Breast Cancer cells via Inhibiting miR-513b-5p
title_sort	n6-methyladenosine-regulated linc00675 suppress the proliferation, migration and invasion of breast cancer cells via inhibiting mir-513b-5p
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/47285d3a21764eb58817f58a18ea754b
work_keys_str_mv	AT shenglanfan n6methyladenosineregulatedlinc00675suppresstheproliferationmigrationandinvasionofbreastcancercellsviainhibitingmir513b5p AT lipingwang n6methyladenosineregulatedlinc00675suppresstheproliferationmigrationandinvasionofbreastcancercellsviainhibitingmir513b5p
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N6-Methyladenosine-regulated LINC00675 Suppress the Proliferation, Migration and Invasion of Breast Cancer cells via Inhibiting miR-513b-5p

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