Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel

SthK, a cyclic nucleotide-gated ion channel from Spirochaeta thermophila activates slowly upon cAMP increase. Here, authors investigate cAMP-induced activation in purified SthK channels using stopped-flow assays and enzymatic catalysis and reveal that the cis/trans conformation of a conserved prolin...

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Autores principales:	Philipp A. M. Schmidpeter, Jan Rheinberger, Crina M. Nimigean
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/472b03d2ec91449c83984880c8a21003
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spelling	oai:doaj.org-article:472b03d2ec91449c83984880c8a210032021-12-02T13:27:23ZProlyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel10.1038/s41467-020-20104-42041-1723https://doaj.org/article/472b03d2ec91449c83984880c8a210032020-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-20104-4https://doaj.org/toc/2041-1723SthK, a cyclic nucleotide-gated ion channel from Spirochaeta thermophila activates slowly upon cAMP increase. Here, authors investigate cAMP-induced activation in purified SthK channels using stopped-flow assays and enzymatic catalysis and reveal that the cis/trans conformation of a conserved proline in the cyclic nucleotide-binding domain determines the activation kinetics of SthK.Philipp A. M. SchmidpeterJan RheinbergerCrina M. NimigeanNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Philipp A. M. Schmidpeter Jan Rheinberger Crina M. Nimigean Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel
description	SthK, a cyclic nucleotide-gated ion channel from Spirochaeta thermophila activates slowly upon cAMP increase. Here, authors investigate cAMP-induced activation in purified SthK channels using stopped-flow assays and enzymatic catalysis and reveal that the cis/trans conformation of a conserved proline in the cyclic nucleotide-binding domain determines the activation kinetics of SthK.
format	article
author	Philipp A. M. Schmidpeter Jan Rheinberger Crina M. Nimigean
author_facet	Philipp A. M. Schmidpeter Jan Rheinberger Crina M. Nimigean
author_sort	Philipp A. M. Schmidpeter
title	Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel
title_short	Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel
title_full	Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel
title_fullStr	Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel
title_full_unstemmed	Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel
title_sort	prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/472b03d2ec91449c83984880c8a21003
work_keys_str_mv	AT philippamschmidpeter prolylisomerizationcontrolsactivationkineticsofacyclicnucleotidegatedionchannel AT janrheinberger prolylisomerizationcontrolsactivationkineticsofacyclicnucleotidegatedionchannel AT crinamnimigean prolylisomerizationcontrolsactivationkineticsofacyclicnucleotidegatedionchannel
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Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel

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