Direct induction of chondrogenic cells from human dermal fibroblast culture by defined factors.

The repair of large cartilage defects with hyaline cartilage continues to be a challenging clinical issue. We recently reported that the forced expression of two reprogramming factors (c-Myc and Klf4) and one chondrogenic factor (SOX9) can induce chondrogenic cells from mouse dermal fibroblast cultu...

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Autores principales:	Hidetatsu Outani, Minoru Okada, Akihiro Yamashita, Kanako Nakagawa, Hideki Yoshikawa, Noriyuki Tsumaki
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/47374c2ce230426a9ab4d2f778013157
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spelling	oai:doaj.org-article:47374c2ce230426a9ab4d2f7780131572021-11-18T08:50:45ZDirect induction of chondrogenic cells from human dermal fibroblast culture by defined factors.1932-620310.1371/journal.pone.0077365https://doaj.org/article/47374c2ce230426a9ab4d2f7780131572013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24146984/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The repair of large cartilage defects with hyaline cartilage continues to be a challenging clinical issue. We recently reported that the forced expression of two reprogramming factors (c-Myc and Klf4) and one chondrogenic factor (SOX9) can induce chondrogenic cells from mouse dermal fibroblast culture without going through a pluripotent state. We here generated induced chondrogenic (iChon) cells from human dermal fibroblast (HDF) culture with the same factors. We developed a chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells. The human iChon cells expressed marker genes for chondrocytes but not fibroblasts, and were derived from non-chondrogenic COL11A2-negative cells. The human iChon cells formed cartilage but not tumors in nude mice. This approach could lead to the preparation of cartilage directly from skin in human, without going through pluripotent stem cells.Hidetatsu OutaniMinoru OkadaAkihiro YamashitaKanako NakagawaHideki YoshikawaNoriyuki TsumakiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e77365 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hidetatsu Outani Minoru Okada Akihiro Yamashita Kanako Nakagawa Hideki Yoshikawa Noriyuki Tsumaki Direct induction of chondrogenic cells from human dermal fibroblast culture by defined factors.
description	The repair of large cartilage defects with hyaline cartilage continues to be a challenging clinical issue. We recently reported that the forced expression of two reprogramming factors (c-Myc and Klf4) and one chondrogenic factor (SOX9) can induce chondrogenic cells from mouse dermal fibroblast culture without going through a pluripotent state. We here generated induced chondrogenic (iChon) cells from human dermal fibroblast (HDF) culture with the same factors. We developed a chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells. The human iChon cells expressed marker genes for chondrocytes but not fibroblasts, and were derived from non-chondrogenic COL11A2-negative cells. The human iChon cells formed cartilage but not tumors in nude mice. This approach could lead to the preparation of cartilage directly from skin in human, without going through pluripotent stem cells.
format	article
author	Hidetatsu Outani Minoru Okada Akihiro Yamashita Kanako Nakagawa Hideki Yoshikawa Noriyuki Tsumaki
author_facet	Hidetatsu Outani Minoru Okada Akihiro Yamashita Kanako Nakagawa Hideki Yoshikawa Noriyuki Tsumaki
author_sort	Hidetatsu Outani
title	Direct induction of chondrogenic cells from human dermal fibroblast culture by defined factors.
title_short	Direct induction of chondrogenic cells from human dermal fibroblast culture by defined factors.
title_full	Direct induction of chondrogenic cells from human dermal fibroblast culture by defined factors.
title_fullStr	Direct induction of chondrogenic cells from human dermal fibroblast culture by defined factors.
title_full_unstemmed	Direct induction of chondrogenic cells from human dermal fibroblast culture by defined factors.
title_sort	direct induction of chondrogenic cells from human dermal fibroblast culture by defined factors.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/47374c2ce230426a9ab4d2f778013157
work_keys_str_mv	AT hidetatsuoutani directinductionofchondrogeniccellsfromhumandermalfibroblastculturebydefinedfactors AT minoruokada directinductionofchondrogeniccellsfromhumandermalfibroblastculturebydefinedfactors AT akihiroyamashita directinductionofchondrogeniccellsfromhumandermalfibroblastculturebydefinedfactors AT kanakonakagawa directinductionofchondrogeniccellsfromhumandermalfibroblastculturebydefinedfactors AT hidekiyoshikawa directinductionofchondrogeniccellsfromhumandermalfibroblastculturebydefinedfactors AT noriyukitsumaki directinductionofchondrogeniccellsfromhumandermalfibroblastculturebydefinedfactors
_version_	1718421257863561216

Direct induction of chondrogenic cells from human dermal fibroblast culture by defined factors.

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