Surgical site peptidylarginine deaminase 4 (PAD4), a biomarker of NETosis, correlates with insulin resistance in total joint arthroplasty patients: A preliminary report.

While obesity and insulin resistance are known risk factors for wound complications after total joint arthroplasty (TJA), the biologic causes remain to be elucidated. Recently, neutrophil extracellular trap formation (NETosis) was identified as a mediator of delayed wound healing in insulin resistan...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Vitor F Martins, Christopher R Dobson, Maedha Begur, Jesal Parekh, Scott T Ball, Francis Gonzalez, Jan M Hughes-Austin, Simon Schenk
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/473d57937438404f88697861fea8cb8c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:473d57937438404f88697861fea8cb8c
record_format	dspace
spelling	oai:doaj.org-article:473d57937438404f88697861fea8cb8c2021-12-02T20:11:27ZSurgical site peptidylarginine deaminase 4 (PAD4), a biomarker of NETosis, correlates with insulin resistance in total joint arthroplasty patients: A preliminary report.1932-620310.1371/journal.pone.0245594https://doaj.org/article/473d57937438404f88697861fea8cb8c2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0245594https://doaj.org/toc/1932-6203While obesity and insulin resistance are known risk factors for wound complications after total joint arthroplasty (TJA), the biologic causes remain to be elucidated. Recently, neutrophil extracellular trap formation (NETosis) was identified as a mediator of delayed wound healing in insulin resistant states. Herein, we explored the relationship between obesity, insulin resistance and biomarkers of NET formation in TJA subjects. We enrolled 14 obese (body mass index [BMI]≥30 kg/m2), and 15 lean (BMI<30 kg/m2) subjects undergoing primary knee or hip TJA. On the day of surgery, skeletal muscle proximal to the operated joint and plasma were collected. Protein abundance of NETosis biomarkers, peptidylarginine deaminase 4 (PAD4) and neutrophil elastase (NE) were assessed in skeletal muscle by immunoblotting and metabolic parameters (glucose, insulin, triglycerides, free fatty acids) and cell-free double-stranded DNA (cf-dsDNA) were assessed in plasma and were correlated with obesity and insulin resistance (as measured by the homeostatic model assessment for insulin resistance). When comparing lean and obese subjects, there were no significant differences in plasma cf-dsDNA or skeletal muscle NE or PAD4 abundance. In contrast, skeletal muscle PAD4 abundance, but not NE or plasma cf-dsDNA, was positively correlated with insulin resistance. Compared to insulin sensitive subjects, insulin resistant TJA subjects have higher expression of PAD4 at the surgical site and therefore may have higher rates of NET formation, which may lead to delayed surgical site wound healing.Vitor F MartinsChristopher R DobsonMaedha BegurJesal ParekhScott T BallFrancis GonzalezJan M Hughes-AustinSimon SchenkPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 1, p e0245594 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vitor F Martins Christopher R Dobson Maedha Begur Jesal Parekh Scott T Ball Francis Gonzalez Jan M Hughes-Austin Simon Schenk Surgical site peptidylarginine deaminase 4 (PAD4), a biomarker of NETosis, correlates with insulin resistance in total joint arthroplasty patients: A preliminary report.
description	While obesity and insulin resistance are known risk factors for wound complications after total joint arthroplasty (TJA), the biologic causes remain to be elucidated. Recently, neutrophil extracellular trap formation (NETosis) was identified as a mediator of delayed wound healing in insulin resistant states. Herein, we explored the relationship between obesity, insulin resistance and biomarkers of NET formation in TJA subjects. We enrolled 14 obese (body mass index [BMI]≥30 kg/m2), and 15 lean (BMI<30 kg/m2) subjects undergoing primary knee or hip TJA. On the day of surgery, skeletal muscle proximal to the operated joint and plasma were collected. Protein abundance of NETosis biomarkers, peptidylarginine deaminase 4 (PAD4) and neutrophil elastase (NE) were assessed in skeletal muscle by immunoblotting and metabolic parameters (glucose, insulin, triglycerides, free fatty acids) and cell-free double-stranded DNA (cf-dsDNA) were assessed in plasma and were correlated with obesity and insulin resistance (as measured by the homeostatic model assessment for insulin resistance). When comparing lean and obese subjects, there were no significant differences in plasma cf-dsDNA or skeletal muscle NE or PAD4 abundance. In contrast, skeletal muscle PAD4 abundance, but not NE or plasma cf-dsDNA, was positively correlated with insulin resistance. Compared to insulin sensitive subjects, insulin resistant TJA subjects have higher expression of PAD4 at the surgical site and therefore may have higher rates of NET formation, which may lead to delayed surgical site wound healing.
format	article
author	Vitor F Martins Christopher R Dobson Maedha Begur Jesal Parekh Scott T Ball Francis Gonzalez Jan M Hughes-Austin Simon Schenk
author_facet	Vitor F Martins Christopher R Dobson Maedha Begur Jesal Parekh Scott T Ball Francis Gonzalez Jan M Hughes-Austin Simon Schenk
author_sort	Vitor F Martins
title	Surgical site peptidylarginine deaminase 4 (PAD4), a biomarker of NETosis, correlates with insulin resistance in total joint arthroplasty patients: A preliminary report.
title_short	Surgical site peptidylarginine deaminase 4 (PAD4), a biomarker of NETosis, correlates with insulin resistance in total joint arthroplasty patients: A preliminary report.
title_full	Surgical site peptidylarginine deaminase 4 (PAD4), a biomarker of NETosis, correlates with insulin resistance in total joint arthroplasty patients: A preliminary report.
title_fullStr	Surgical site peptidylarginine deaminase 4 (PAD4), a biomarker of NETosis, correlates with insulin resistance in total joint arthroplasty patients: A preliminary report.
title_full_unstemmed	Surgical site peptidylarginine deaminase 4 (PAD4), a biomarker of NETosis, correlates with insulin resistance in total joint arthroplasty patients: A preliminary report.
title_sort	surgical site peptidylarginine deaminase 4 (pad4), a biomarker of netosis, correlates with insulin resistance in total joint arthroplasty patients: a preliminary report.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/473d57937438404f88697861fea8cb8c
work_keys_str_mv	AT vitorfmartins surgicalsitepeptidylargininedeaminase4pad4abiomarkerofnetosiscorrelateswithinsulinresistanceintotaljointarthroplastypatientsapreliminaryreport AT christopherrdobson surgicalsitepeptidylargininedeaminase4pad4abiomarkerofnetosiscorrelateswithinsulinresistanceintotaljointarthroplastypatientsapreliminaryreport AT maedhabegur surgicalsitepeptidylargininedeaminase4pad4abiomarkerofnetosiscorrelateswithinsulinresistanceintotaljointarthroplastypatientsapreliminaryreport AT jesalparekh surgicalsitepeptidylargininedeaminase4pad4abiomarkerofnetosiscorrelateswithinsulinresistanceintotaljointarthroplastypatientsapreliminaryreport AT scotttball surgicalsitepeptidylargininedeaminase4pad4abiomarkerofnetosiscorrelateswithinsulinresistanceintotaljointarthroplastypatientsapreliminaryreport AT francisgonzalez surgicalsitepeptidylargininedeaminase4pad4abiomarkerofnetosiscorrelateswithinsulinresistanceintotaljointarthroplastypatientsapreliminaryreport AT janmhughesaustin surgicalsitepeptidylargininedeaminase4pad4abiomarkerofnetosiscorrelateswithinsulinresistanceintotaljointarthroplastypatientsapreliminaryreport AT simonschenk surgicalsitepeptidylargininedeaminase4pad4abiomarkerofnetosiscorrelateswithinsulinresistanceintotaljointarthroplastypatientsapreliminaryreport
_version_	1718374880620052480

Surgical site peptidylarginine deaminase 4 (PAD4), a biomarker of NETosis, correlates with insulin resistance in total joint arthroplasty patients: A preliminary report.

Ejemplares similares