Male microchimerism at high levels in peripheral blood mononuclear cells from women with end stage renal disease before kidney transplantation.

Patients with end stage renal diseases (ESRD) are generally tested for donor chimerism after kidney transplantation for tolerance mechanism purposes. But, to our knowledge, no data are available on natural and/or iatrogenic microchimerism (Mc), deriving from pregnancy and/or blood transfusion, acqui...

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Autores principales:	Laetitia Albano, Justyna M Rak, Doua F Azzouz, Elisabeth Cassuto-Viguier, Jean Gugenheim, Nathalie C Lambert
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/47401512e69e49c5b9ce6a6d165584cc
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spelling	oai:doaj.org-article:47401512e69e49c5b9ce6a6d165584cc2021-11-18T07:26:09ZMale microchimerism at high levels in peripheral blood mononuclear cells from women with end stage renal disease before kidney transplantation.1932-620310.1371/journal.pone.0032248https://doaj.org/article/47401512e69e49c5b9ce6a6d165584cc2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22403639/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Patients with end stage renal diseases (ESRD) are generally tested for donor chimerism after kidney transplantation for tolerance mechanism purposes. But, to our knowledge, no data are available on natural and/or iatrogenic microchimerism (Mc), deriving from pregnancy and/or blood transfusion, acquired prior to transplantation. In this context, we tested the prevalence of male Mc using a real time PCR assay for DYS14, a Y-chromosome specific sequence, in peripheral blood mononuclear cells (PBMC) from 55 women with ESRD, prior to their first kidney transplantation, and compared them with results from 82 healthy women. Male Mc was also quantified in 5 native kidney biopsies obtained two to four years prior to blood testing and in PBMC from 8 women collected after female kidney transplantation, several years after the initial blood testing. Women with ESRD showed statistically higher frequencies (62%) and quantities (98 genome equivalent cells per million of host cells, gEq/M) of male Mc in their PBMC than healthy women (16% and 0.3 gEq/M, p<0.00001 and p = 0.0005 respectively). Male Mc was increased in women with ESRD whether they had or not a history of male pregnancy and/or of blood transfusion. Three out of five renal biopsies obtained a few years prior to the blood test also contained Mc, but no correlation could be established between earlier Mc in a kidney and later presence in PBMC. Finally, several years after female kidney transplantation, male Mc was totally cleared from PBMC in all women tested but one. This intriguing and striking initial result of natural and iatrogenic male Mc persistence in peripheral blood from women with ESRD raises several hypotheses for the possible role of these cells in renal diseases. Further studies are needed to elucidate mechanisms of recruitment and persistence of Mc in women with ESRD.Laetitia AlbanoJustyna M RakDoua F AzzouzElisabeth Cassuto-ViguierJean GugenheimNathalie C LambertPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e32248 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laetitia Albano Justyna M Rak Doua F Azzouz Elisabeth Cassuto-Viguier Jean Gugenheim Nathalie C Lambert Male microchimerism at high levels in peripheral blood mononuclear cells from women with end stage renal disease before kidney transplantation.
description	Patients with end stage renal diseases (ESRD) are generally tested for donor chimerism after kidney transplantation for tolerance mechanism purposes. But, to our knowledge, no data are available on natural and/or iatrogenic microchimerism (Mc), deriving from pregnancy and/or blood transfusion, acquired prior to transplantation. In this context, we tested the prevalence of male Mc using a real time PCR assay for DYS14, a Y-chromosome specific sequence, in peripheral blood mononuclear cells (PBMC) from 55 women with ESRD, prior to their first kidney transplantation, and compared them with results from 82 healthy women. Male Mc was also quantified in 5 native kidney biopsies obtained two to four years prior to blood testing and in PBMC from 8 women collected after female kidney transplantation, several years after the initial blood testing. Women with ESRD showed statistically higher frequencies (62%) and quantities (98 genome equivalent cells per million of host cells, gEq/M) of male Mc in their PBMC than healthy women (16% and 0.3 gEq/M, p<0.00001 and p = 0.0005 respectively). Male Mc was increased in women with ESRD whether they had or not a history of male pregnancy and/or of blood transfusion. Three out of five renal biopsies obtained a few years prior to the blood test also contained Mc, but no correlation could be established between earlier Mc in a kidney and later presence in PBMC. Finally, several years after female kidney transplantation, male Mc was totally cleared from PBMC in all women tested but one. This intriguing and striking initial result of natural and iatrogenic male Mc persistence in peripheral blood from women with ESRD raises several hypotheses for the possible role of these cells in renal diseases. Further studies are needed to elucidate mechanisms of recruitment and persistence of Mc in women with ESRD.
format	article
author	Laetitia Albano Justyna M Rak Doua F Azzouz Elisabeth Cassuto-Viguier Jean Gugenheim Nathalie C Lambert
author_facet	Laetitia Albano Justyna M Rak Doua F Azzouz Elisabeth Cassuto-Viguier Jean Gugenheim Nathalie C Lambert
author_sort	Laetitia Albano
title	Male microchimerism at high levels in peripheral blood mononuclear cells from women with end stage renal disease before kidney transplantation.
title_short	Male microchimerism at high levels in peripheral blood mononuclear cells from women with end stage renal disease before kidney transplantation.
title_full	Male microchimerism at high levels in peripheral blood mononuclear cells from women with end stage renal disease before kidney transplantation.
title_fullStr	Male microchimerism at high levels in peripheral blood mononuclear cells from women with end stage renal disease before kidney transplantation.
title_full_unstemmed	Male microchimerism at high levels in peripheral blood mononuclear cells from women with end stage renal disease before kidney transplantation.
title_sort	male microchimerism at high levels in peripheral blood mononuclear cells from women with end stage renal disease before kidney transplantation.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/47401512e69e49c5b9ce6a6d165584cc
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_version_	1718423470882160640

Male microchimerism at high levels in peripheral blood mononuclear cells from women with end stage renal disease before kidney transplantation.

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