In vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing

Abstract Wound healing and the management of chronic wounds represent a significant burden on the NHS. Members of the suppressor of cytokine signalling (SOCS) family have been implicated in the regulation of a range of cellular processes. The current study aims to explore the importance of SOCS-3 an...

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Autores principales:	Yi Feng, Andrew J. Sanders, Liam D. Morgan, Sioned Owen, Fiona Ruge, Keith G. Harding, Wen G. Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4741a37b5c9f48cd91e0de6edfa609ad
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spelling	oai:doaj.org-article:4741a37b5c9f48cd91e0de6edfa609ad2021-12-02T11:52:57ZIn vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing10.1038/s41598-017-06886-62045-2322https://doaj.org/article/4741a37b5c9f48cd91e0de6edfa609ad2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06886-6https://doaj.org/toc/2045-2322Abstract Wound healing and the management of chronic wounds represent a significant burden on the NHS. Members of the suppressor of cytokine signalling (SOCS) family have been implicated in the regulation of a range of cellular processes. The current study aims to explore the importance of SOCS-3 and SOCS-4 in regulating cellular traits associated with wound healing. SOCS-3 over-expression and SOCS-4 knockdown mutant lines were generated and verified using q-PCR and western blotting in human keratinocytes (HaCaT) and endothelial cells (HECV). Over-expression of SOCS-3 resulted in a significantly reduced proliferative rate in HaCaT keratinocytes and also enhanced the tubule formation capacity of HECV cells. SOCS-4 knockdown significantly reduced HaCaT migration and HECV cell tubule formation. Suppression of SOCS-4 influenced the responsiveness of HaCaT and HECV cells to EGF and TGFβ and resulted in a dysregulation of phospho-protein expression in HaCaT cells. SOCS-3 and SOCS-4 appear to play regulatory roles in a number of keratinocyte and endothelial cellular traits associated with the wound healing process and may also be able to regulate the responsiveness of these cells to EGF and TGFβ. This implies a potential regulatory role in the wound healing process and, thus highlights their potential as novel therapies.Yi FengAndrew J. SandersLiam D. MorganSioned OwenFiona RugeKeith G. HardingWen G. JiangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yi Feng Andrew J. Sanders Liam D. Morgan Sioned Owen Fiona Ruge Keith G. Harding Wen G. Jiang In vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing
description	Abstract Wound healing and the management of chronic wounds represent a significant burden on the NHS. Members of the suppressor of cytokine signalling (SOCS) family have been implicated in the regulation of a range of cellular processes. The current study aims to explore the importance of SOCS-3 and SOCS-4 in regulating cellular traits associated with wound healing. SOCS-3 over-expression and SOCS-4 knockdown mutant lines were generated and verified using q-PCR and western blotting in human keratinocytes (HaCaT) and endothelial cells (HECV). Over-expression of SOCS-3 resulted in a significantly reduced proliferative rate in HaCaT keratinocytes and also enhanced the tubule formation capacity of HECV cells. SOCS-4 knockdown significantly reduced HaCaT migration and HECV cell tubule formation. Suppression of SOCS-4 influenced the responsiveness of HaCaT and HECV cells to EGF and TGFβ and resulted in a dysregulation of phospho-protein expression in HaCaT cells. SOCS-3 and SOCS-4 appear to play regulatory roles in a number of keratinocyte and endothelial cellular traits associated with the wound healing process and may also be able to regulate the responsiveness of these cells to EGF and TGFβ. This implies a potential regulatory role in the wound healing process and, thus highlights their potential as novel therapies.
format	article
author	Yi Feng Andrew J. Sanders Liam D. Morgan Sioned Owen Fiona Ruge Keith G. Harding Wen G. Jiang
author_facet	Yi Feng Andrew J. Sanders Liam D. Morgan Sioned Owen Fiona Ruge Keith G. Harding Wen G. Jiang
author_sort	Yi Feng
title	In vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing
title_short	In vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing
title_full	In vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing
title_fullStr	In vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing
title_full_unstemmed	In vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing
title_sort	in vitro significance of socs-3 and socs-4 and potential mechanistic links to wound healing
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4741a37b5c9f48cd91e0de6edfa609ad
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In vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing

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