The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of <i>Haematococcus pluvialis</i> (Volvocales, Chlorophyta)

Unicellular volvocalean green algal <i>Haematococcus pluvialis</i>, known as astaxanthin rich microalgae, transforms into aplanospore stage from the flagellate stage when exposed to the stress environments. However, the mechanism of the formation of aplanospore cell wall, which hinders t...

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Autores principales:	Chunli Guo, Rui Mei, Muhammad Anwar, Di Zhao, Chengxiang Lan, Yanan Jiang, Jieyi Zhuang, Chaogang Wang, Zhangli Hu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Haematococcus pluvialis</i> cell wall mannose genes mechanism Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4743ebf8f47942f5a51a0f0930c4508f
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spelling	oai:doaj.org-article:4743ebf8f47942f5a51a0f0930c4508f2021-11-25T18:20:28ZThe Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of <i>Haematococcus pluvialis</i> (Volvocales, Chlorophyta)10.3390/metabo111107252218-1989https://doaj.org/article/4743ebf8f47942f5a51a0f0930c4508f2021-10-01T00:00:00Zhttps://www.mdpi.com/2218-1989/11/11/725https://doaj.org/toc/2218-1989Unicellular volvocalean green algal <i>Haematococcus pluvialis</i>, known as astaxanthin rich microalgae, transforms into aplanospore stage from the flagellate stage when exposed to the stress environments. However, the mechanism of the formation of aplanospore cell wall, which hinders the extraction of astaxanthin and the genetic manipulation is still unclear. In this study, the cell wall components under salicylic acid and high light stresses were explored, and cellulose was considered the main component in the flagellates, which changed gradually into mannose in the aplanospore stages. During the period, the genes related to the cellulose and mannose metabolisms were identified based on the RNA-seq data, which presented a similar expression pattern. The positive correlations were observed among these studied genes by Pearson Correlation (PC) analysis, indicating the coordination between pathways of cellulose and mannose metabolism. The study firstly explored the formation mechanism of aplanospore cell wall, which might be of scientific significance in the study of <i>H. pluvialis</i>.Chunli GuoRui MeiMuhammad AnwarDi ZhaoChengxiang LanYanan JiangJieyi ZhuangChaogang WangZhangli HuMDPI AGarticle<i>Haematococcus pluvialis</i>cell wallmannosegenesmechanismMicrobiologyQR1-502ENMetabolites, Vol 11, Iss 725, p 725 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Haematococcus pluvialis</i> cell wall mannose genes mechanism Microbiology QR1-502
spellingShingle	<i>Haematococcus pluvialis</i> cell wall mannose genes mechanism Microbiology QR1-502 Chunli Guo Rui Mei Muhammad Anwar Di Zhao Chengxiang Lan Yanan Jiang Jieyi Zhuang Chaogang Wang Zhangli Hu The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of <i>Haematococcus pluvialis</i> (Volvocales, Chlorophyta)
description	Unicellular volvocalean green algal <i>Haematococcus pluvialis</i>, known as astaxanthin rich microalgae, transforms into aplanospore stage from the flagellate stage when exposed to the stress environments. However, the mechanism of the formation of aplanospore cell wall, which hinders the extraction of astaxanthin and the genetic manipulation is still unclear. In this study, the cell wall components under salicylic acid and high light stresses were explored, and cellulose was considered the main component in the flagellates, which changed gradually into mannose in the aplanospore stages. During the period, the genes related to the cellulose and mannose metabolisms were identified based on the RNA-seq data, which presented a similar expression pattern. The positive correlations were observed among these studied genes by Pearson Correlation (PC) analysis, indicating the coordination between pathways of cellulose and mannose metabolism. The study firstly explored the formation mechanism of aplanospore cell wall, which might be of scientific significance in the study of <i>H. pluvialis</i>.
format	article
author	Chunli Guo Rui Mei Muhammad Anwar Di Zhao Chengxiang Lan Yanan Jiang Jieyi Zhuang Chaogang Wang Zhangli Hu
author_facet	Chunli Guo Rui Mei Muhammad Anwar Di Zhao Chengxiang Lan Yanan Jiang Jieyi Zhuang Chaogang Wang Zhangli Hu
author_sort	Chunli Guo
title	The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of <i>Haematococcus pluvialis</i> (Volvocales, Chlorophyta)
title_short	The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of <i>Haematococcus pluvialis</i> (Volvocales, Chlorophyta)
title_full	The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of <i>Haematococcus pluvialis</i> (Volvocales, Chlorophyta)
title_fullStr	The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of <i>Haematococcus pluvialis</i> (Volvocales, Chlorophyta)
title_full_unstemmed	The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of <i>Haematococcus pluvialis</i> (Volvocales, Chlorophyta)
title_sort	functionally characterization of putative genes involved in the formation of mannose in the aplanospore cell wall of <i>haematococcus pluvialis</i> (volvocales, chlorophyta)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4743ebf8f47942f5a51a0f0930c4508f
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The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of <i>Haematococcus pluvialis</i> (Volvocales, Chlorophyta)

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