Mitotic spindle assembly around RCC1-coated beads in Xenopus egg extracts.

During cell division the genetic material on chromosomes is distributed to daughter cells by a dynamic microtubule structure called the mitotic spindle. Here we establish a reconstitution system to assess the contribution of individual chromosome proteins to mitotic spindle formation around single 1...

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Autores principales:	David Halpin, Petr Kalab, Jay Wang, Karsten Weis, Rebecca Heald
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/474ae2bb076643d1a537e623724b970d
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spelling	oai:doaj.org-article:474ae2bb076643d1a537e623724b970d2021-11-18T05:36:50ZMitotic spindle assembly around RCC1-coated beads in Xenopus egg extracts.1544-91731545-788510.1371/journal.pbio.1001225https://doaj.org/article/474ae2bb076643d1a537e623724b970d2011-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22215983/pdf/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885During cell division the genetic material on chromosomes is distributed to daughter cells by a dynamic microtubule structure called the mitotic spindle. Here we establish a reconstitution system to assess the contribution of individual chromosome proteins to mitotic spindle formation around single 10 µm diameter porous glass beads in Xenopus egg extracts. We find that Regulator of Chromosome Condensation 1 (RCC1), the Guanine Nucleotide Exchange Factor (GEF) for the small GTPase Ran, can induce bipolar spindle formation. Remarkably, RCC1 beads oscillate within spindles from pole to pole, a behavior that could be converted to a more typical, stable association by the addition of a kinesin together with RCC1. These results identify two activities sufficient to mimic chromatin-mediated spindle assembly, and establish a foundation for future experiments to reconstitute spindle assembly entirely from purified components.David HalpinPetr KalabJay WangKarsten WeisRebecca HealdPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 9, Iss 12, p e1001225 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 David Halpin Petr Kalab Jay Wang Karsten Weis Rebecca Heald Mitotic spindle assembly around RCC1-coated beads in Xenopus egg extracts.
description	During cell division the genetic material on chromosomes is distributed to daughter cells by a dynamic microtubule structure called the mitotic spindle. Here we establish a reconstitution system to assess the contribution of individual chromosome proteins to mitotic spindle formation around single 10 µm diameter porous glass beads in Xenopus egg extracts. We find that Regulator of Chromosome Condensation 1 (RCC1), the Guanine Nucleotide Exchange Factor (GEF) for the small GTPase Ran, can induce bipolar spindle formation. Remarkably, RCC1 beads oscillate within spindles from pole to pole, a behavior that could be converted to a more typical, stable association by the addition of a kinesin together with RCC1. These results identify two activities sufficient to mimic chromatin-mediated spindle assembly, and establish a foundation for future experiments to reconstitute spindle assembly entirely from purified components.
format	article
author	David Halpin Petr Kalab Jay Wang Karsten Weis Rebecca Heald
author_facet	David Halpin Petr Kalab Jay Wang Karsten Weis Rebecca Heald
author_sort	David Halpin
title	Mitotic spindle assembly around RCC1-coated beads in Xenopus egg extracts.
title_short	Mitotic spindle assembly around RCC1-coated beads in Xenopus egg extracts.
title_full	Mitotic spindle assembly around RCC1-coated beads in Xenopus egg extracts.
title_fullStr	Mitotic spindle assembly around RCC1-coated beads in Xenopus egg extracts.
title_full_unstemmed	Mitotic spindle assembly around RCC1-coated beads in Xenopus egg extracts.
title_sort	mitotic spindle assembly around rcc1-coated beads in xenopus egg extracts.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/474ae2bb076643d1a537e623724b970d
work_keys_str_mv	AT davidhalpin mitoticspindleassemblyaroundrcc1coatedbeadsinxenopuseggextracts AT petrkalab mitoticspindleassemblyaroundrcc1coatedbeadsinxenopuseggextracts AT jaywang mitoticspindleassemblyaroundrcc1coatedbeadsinxenopuseggextracts AT karstenweis mitoticspindleassemblyaroundrcc1coatedbeadsinxenopuseggextracts AT rebeccaheald mitoticspindleassemblyaroundrcc1coatedbeadsinxenopuseggextracts
_version_	1718424846180810752

Mitotic spindle assembly around RCC1-coated beads in Xenopus egg extracts.

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