Successful extraction of insect DNA from recent copal inclusions: limits and perspectives

Abstract Insects entombed in copal, the sub-fossilized resin precursor of amber, represent a potential source of genetic data for extinct and extant, but endangered or elusive, species. Despite several studies demonstrated that it is not possible to recover endogenous DNA from insect inclusions, the...

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Autores principales:	Alessandra Modi, Chiara Vergata, Cristina Zilli, Chiara Vischioni, Stefania Vai, Guidantonio Malagoli Tagliazucchi, Martina Lari, David Caramelli, Cristian Taccioli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/474f74f37fda4674beda38f0877613ff
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spelling	oai:doaj.org-article:474f74f37fda4674beda38f0877613ff2021-12-02T13:24:15ZSuccessful extraction of insect DNA from recent copal inclusions: limits and perspectives10.1038/s41598-021-86058-92045-2322https://doaj.org/article/474f74f37fda4674beda38f0877613ff2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86058-9https://doaj.org/toc/2045-2322Abstract Insects entombed in copal, the sub-fossilized resin precursor of amber, represent a potential source of genetic data for extinct and extant, but endangered or elusive, species. Despite several studies demonstrated that it is not possible to recover endogenous DNA from insect inclusions, the preservation of biomolecules in fossilized resins samples is still under debate. In this study, we tested the possibility of obtaining endogenous ancient DNA (aDNA) molecules from insects preserved in copal, applying experimental protocols specifically designed for aDNA recovery. We were able to extract endogenous DNA molecules from one of the two samples analyzed, and to identify the taxonomic status of the specimen. Even if the sample was found well protected from external contaminants, the recovered DNA was low concentrated and extremely degraded, compared to the sample age. We conclude that it is possible to obtain genomic data from resin-entombed organisms, although we discourage aDNA analysis because of the destructive method of extraction protocols and the non-reproducibility of the results.Alessandra ModiChiara VergataCristina ZilliChiara VischioniStefania VaiGuidantonio Malagoli TagliazucchiMartina LariDavid CaramelliCristian TaccioliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alessandra Modi Chiara Vergata Cristina Zilli Chiara Vischioni Stefania Vai Guidantonio Malagoli Tagliazucchi Martina Lari David Caramelli Cristian Taccioli Successful extraction of insect DNA from recent copal inclusions: limits and perspectives
description	Abstract Insects entombed in copal, the sub-fossilized resin precursor of amber, represent a potential source of genetic data for extinct and extant, but endangered or elusive, species. Despite several studies demonstrated that it is not possible to recover endogenous DNA from insect inclusions, the preservation of biomolecules in fossilized resins samples is still under debate. In this study, we tested the possibility of obtaining endogenous ancient DNA (aDNA) molecules from insects preserved in copal, applying experimental protocols specifically designed for aDNA recovery. We were able to extract endogenous DNA molecules from one of the two samples analyzed, and to identify the taxonomic status of the specimen. Even if the sample was found well protected from external contaminants, the recovered DNA was low concentrated and extremely degraded, compared to the sample age. We conclude that it is possible to obtain genomic data from resin-entombed organisms, although we discourage aDNA analysis because of the destructive method of extraction protocols and the non-reproducibility of the results.
format	article
author	Alessandra Modi Chiara Vergata Cristina Zilli Chiara Vischioni Stefania Vai Guidantonio Malagoli Tagliazucchi Martina Lari David Caramelli Cristian Taccioli
author_facet	Alessandra Modi Chiara Vergata Cristina Zilli Chiara Vischioni Stefania Vai Guidantonio Malagoli Tagliazucchi Martina Lari David Caramelli Cristian Taccioli
author_sort	Alessandra Modi
title	Successful extraction of insect DNA from recent copal inclusions: limits and perspectives
title_short	Successful extraction of insect DNA from recent copal inclusions: limits and perspectives
title_full	Successful extraction of insect DNA from recent copal inclusions: limits and perspectives
title_fullStr	Successful extraction of insect DNA from recent copal inclusions: limits and perspectives
title_full_unstemmed	Successful extraction of insect DNA from recent copal inclusions: limits and perspectives
title_sort	successful extraction of insect dna from recent copal inclusions: limits and perspectives
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/474f74f37fda4674beda38f0877613ff
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Successful extraction of insect DNA from recent copal inclusions: limits and perspectives

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