Nicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic beta cells via MIF pathway

Ioana Ilie,1 Razvan Ilie,2 Teodora Mocan,3 Flaviu Tabaran,4 Cornel Iancu,4 Lucian Mocan4 1Department of Endocrinology, 2Department of Microbiology, 3Department of Physiology, 4Third Surgery Clinic, Department of Nanomedicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania...

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Autores principales:	Ilie I, Ilie R, Mocan T, Tabaran F, Iancu C, Mocan L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4756008bcd1b4f489f5ef538ae7bfe99
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spelling	oai:doaj.org-article:4756008bcd1b4f489f5ef538ae7bfe992021-12-02T02:42:33ZNicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic beta cells via MIF pathway1176-91141178-2013https://doaj.org/article/4756008bcd1b4f489f5ef538ae7bfe992013-08-01T00:00:00Zhttp://www.dovepress.com/nicotinamide-functionalized-multiwalled-carbon-nanotubes-increase-insu-a14210https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Ioana Ilie,1 Razvan Ilie,2 Teodora Mocan,3 Flaviu Tabaran,4 Cornel Iancu,4 Lucian Mocan4 1Department of Endocrinology, 2Department of Microbiology, 3Department of Physiology, 4Third Surgery Clinic, Department of Nanomedicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania Abstract: Recent data in the literature support the role of nicotinamide (NA) as a pharmacologic agent that stimulates pancreatic beta-cells to produce insulin in vitro. There are data showing that carbon nanotubes may be useful in initiating and maintaining cellular metabolic responses. This study shows that administration of multiwalled carbon nanotubes (MWCNTs) functionalized with nicotinamide (NA-MWCNTs) leads to significant insulin production compared with individual administration of NA, MWCNTs, and a control solution. Treatment of 1.4E7 cells for 30 minutes with NA-MWCNTs at concentrations ranging from 1 mg/L to 20 mg/L resulted in significantly increased insulin release (0.18 ± 0.026 ng/mL for 1 mg/L, 0.21 ± 0.024 ng/mL for 5 mg/L, and 0.27 ± 0.028 ng/mL for 20 mg/L). Thus, compared with cells treated with NA only (0.1 ± 0.01 ng/mL for 1 mg/L, 0.12 ± 0.017 ng/mL for 5 mg/L, and 0.17 ± 0.01 ng/mL for 20 mg/L) we observed a significant positive effect on insulin release in cells treated with NA-MWCNTs. The results were confirmed using flow cytometry, epifluorescence microscopy combined with immunochemistry staining, and enzyme-linked immunosorbent assay techniques. In addition, using immunofluorescence microscopy techniques, we were able to demonstrate that MWCNTs enhance insulin production via the macrophage migration inhibitory factor pathway. The application and potential of NA combined with MWCNTs as an antidiabetic agent may represent the beginning of a new chapter in the nanomediated treatment of diabetes mellitus. Keywords: carbon nanotubes, NA, insulin-producing cells, insulin, macrophage migration inhibitory factor, diabetes mellitusIlie IIlie RMocan TTabaran FIancu CMocan LDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 3345-3353 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ilie I Ilie R Mocan T Tabaran F Iancu C Mocan L Nicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic beta cells via MIF pathway
description	Ioana Ilie,1 Razvan Ilie,2 Teodora Mocan,3 Flaviu Tabaran,4 Cornel Iancu,4 Lucian Mocan4 1Department of Endocrinology, 2Department of Microbiology, 3Department of Physiology, 4Third Surgery Clinic, Department of Nanomedicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania Abstract: Recent data in the literature support the role of nicotinamide (NA) as a pharmacologic agent that stimulates pancreatic beta-cells to produce insulin in vitro. There are data showing that carbon nanotubes may be useful in initiating and maintaining cellular metabolic responses. This study shows that administration of multiwalled carbon nanotubes (MWCNTs) functionalized with nicotinamide (NA-MWCNTs) leads to significant insulin production compared with individual administration of NA, MWCNTs, and a control solution. Treatment of 1.4E7 cells for 30 minutes with NA-MWCNTs at concentrations ranging from 1 mg/L to 20 mg/L resulted in significantly increased insulin release (0.18 ± 0.026 ng/mL for 1 mg/L, 0.21 ± 0.024 ng/mL for 5 mg/L, and 0.27 ± 0.028 ng/mL for 20 mg/L). Thus, compared with cells treated with NA only (0.1 ± 0.01 ng/mL for 1 mg/L, 0.12 ± 0.017 ng/mL for 5 mg/L, and 0.17 ± 0.01 ng/mL for 20 mg/L) we observed a significant positive effect on insulin release in cells treated with NA-MWCNTs. The results were confirmed using flow cytometry, epifluorescence microscopy combined with immunochemistry staining, and enzyme-linked immunosorbent assay techniques. In addition, using immunofluorescence microscopy techniques, we were able to demonstrate that MWCNTs enhance insulin production via the macrophage migration inhibitory factor pathway. The application and potential of NA combined with MWCNTs as an antidiabetic agent may represent the beginning of a new chapter in the nanomediated treatment of diabetes mellitus. Keywords: carbon nanotubes, NA, insulin-producing cells, insulin, macrophage migration inhibitory factor, diabetes mellitus
format	article
author	Ilie I Ilie R Mocan T Tabaran F Iancu C Mocan L
author_facet	Ilie I Ilie R Mocan T Tabaran F Iancu C Mocan L
author_sort	Ilie I
title	Nicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic beta cells via MIF pathway
title_short	Nicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic beta cells via MIF pathway
title_full	Nicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic beta cells via MIF pathway
title_fullStr	Nicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic beta cells via MIF pathway
title_full_unstemmed	Nicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic beta cells via MIF pathway
title_sort	nicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic beta cells via mif pathway
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/4756008bcd1b4f489f5ef538ae7bfe99
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_version_	1718402214602473472

Nicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic beta cells via MIF pathway

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