Bacterial and fungal endophyte communities in healthy and diseased oilseed rape and their potential for biocontrol of Sclerotinia and Phoma disease

Abstract Phoma stem canker (caused by the ascomycetes Leptosphaeria maculans and Leptosphaeria biglobosa) is an important disease of oilseed rape. Its effect on endophyte communities in roots and shoots and the potential of endophytes to promote growth and control diseases of oilseed rape (OSR) was...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	C. S. Schmidt, L. Mrnka, P. Lovecká, T. Frantík, M. Fenclová, K. Demnerová, M. Vosátka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4757a793f9e840a296cd80c1175db94d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4757a793f9e840a296cd80c1175db94d
record_format	dspace
spelling	oai:doaj.org-article:4757a793f9e840a296cd80c1175db94d2021-12-02T12:11:50ZBacterial and fungal endophyte communities in healthy and diseased oilseed rape and their potential for biocontrol of Sclerotinia and Phoma disease10.1038/s41598-021-81937-72045-2322https://doaj.org/article/4757a793f9e840a296cd80c1175db94d2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81937-7https://doaj.org/toc/2045-2322Abstract Phoma stem canker (caused by the ascomycetes Leptosphaeria maculans and Leptosphaeria biglobosa) is an important disease of oilseed rape. Its effect on endophyte communities in roots and shoots and the potential of endophytes to promote growth and control diseases of oilseed rape (OSR) was investigated. Phoma stem canker had a large effect especially on fungal but also on bacterial endophyte communities. Dominant bacterial genera were Pseudomonas, followed by Enterobacter, Serratia, Stenotrophomonas, Bacillus and Staphylococcus. Achromobacter, Pectobacter and Sphingobacterium were isolated only from diseased plants, though in very small numbers. The fungal genera Cladosporium, Botrytis and Torula were dominant in healthy plants whereas Alternaria, Fusarium and Basidiomycetes (Vishniacozyma, Holtermaniella, Bjerkandera/Thanatephorus) occurred exclusively in diseased plants. Remarkably, Leptosphaeria biglobosa could be isolated in large numbers from shoots of both healthy and diseased plants. Plant growth promoting properties (antioxidative activity, P-solubilisation, production of phytohormones and siderophores) were widespread in OSR endophytes. Although none of the tested bacterial endophytes (Achromobacter, Enterobacter, Pseudomonas, Serratia and Stenotrophomonas) promoted growth of oilseed rape under P-limiting conditions or controlled Phoma disease on oilseed rape cotyledons, they significantly reduced incidence of Sclerotinia disease. In the field, a combined inoculum consisting of Achromobacter piechaudii, two pseudomonads and Stenotrophomonas rhizophila tendencially increased OSR yield and reduced Phoma stem canker.C. S. SchmidtL. MrnkaP. LoveckáT. FrantíkM. FenclováK. DemnerováM. VosátkaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q C. S. Schmidt L. Mrnka P. Lovecká T. Frantík M. Fenclová K. Demnerová M. Vosátka Bacterial and fungal endophyte communities in healthy and diseased oilseed rape and their potential for biocontrol of Sclerotinia and Phoma disease
description	Abstract Phoma stem canker (caused by the ascomycetes Leptosphaeria maculans and Leptosphaeria biglobosa) is an important disease of oilseed rape. Its effect on endophyte communities in roots and shoots and the potential of endophytes to promote growth and control diseases of oilseed rape (OSR) was investigated. Phoma stem canker had a large effect especially on fungal but also on bacterial endophyte communities. Dominant bacterial genera were Pseudomonas, followed by Enterobacter, Serratia, Stenotrophomonas, Bacillus and Staphylococcus. Achromobacter, Pectobacter and Sphingobacterium were isolated only from diseased plants, though in very small numbers. The fungal genera Cladosporium, Botrytis and Torula were dominant in healthy plants whereas Alternaria, Fusarium and Basidiomycetes (Vishniacozyma, Holtermaniella, Bjerkandera/Thanatephorus) occurred exclusively in diseased plants. Remarkably, Leptosphaeria biglobosa could be isolated in large numbers from shoots of both healthy and diseased plants. Plant growth promoting properties (antioxidative activity, P-solubilisation, production of phytohormones and siderophores) were widespread in OSR endophytes. Although none of the tested bacterial endophytes (Achromobacter, Enterobacter, Pseudomonas, Serratia and Stenotrophomonas) promoted growth of oilseed rape under P-limiting conditions or controlled Phoma disease on oilseed rape cotyledons, they significantly reduced incidence of Sclerotinia disease. In the field, a combined inoculum consisting of Achromobacter piechaudii, two pseudomonads and Stenotrophomonas rhizophila tendencially increased OSR yield and reduced Phoma stem canker.
format	article
author	C. S. Schmidt L. Mrnka P. Lovecká T. Frantík M. Fenclová K. Demnerová M. Vosátka
author_facet	C. S. Schmidt L. Mrnka P. Lovecká T. Frantík M. Fenclová K. Demnerová M. Vosátka
author_sort	C. S. Schmidt
title	Bacterial and fungal endophyte communities in healthy and diseased oilseed rape and their potential for biocontrol of Sclerotinia and Phoma disease
title_short	Bacterial and fungal endophyte communities in healthy and diseased oilseed rape and their potential for biocontrol of Sclerotinia and Phoma disease
title_full	Bacterial and fungal endophyte communities in healthy and diseased oilseed rape and their potential for biocontrol of Sclerotinia and Phoma disease
title_fullStr	Bacterial and fungal endophyte communities in healthy and diseased oilseed rape and their potential for biocontrol of Sclerotinia and Phoma disease
title_full_unstemmed	Bacterial and fungal endophyte communities in healthy and diseased oilseed rape and their potential for biocontrol of Sclerotinia and Phoma disease
title_sort	bacterial and fungal endophyte communities in healthy and diseased oilseed rape and their potential for biocontrol of sclerotinia and phoma disease
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4757a793f9e840a296cd80c1175db94d
work_keys_str_mv	AT csschmidt bacterialandfungalendophytecommunitiesinhealthyanddiseasedoilseedrapeandtheirpotentialforbiocontrolofsclerotiniaandphomadisease AT lmrnka bacterialandfungalendophytecommunitiesinhealthyanddiseasedoilseedrapeandtheirpotentialforbiocontrolofsclerotiniaandphomadisease AT plovecka bacterialandfungalendophytecommunitiesinhealthyanddiseasedoilseedrapeandtheirpotentialforbiocontrolofsclerotiniaandphomadisease AT tfrantik bacterialandfungalendophytecommunitiesinhealthyanddiseasedoilseedrapeandtheirpotentialforbiocontrolofsclerotiniaandphomadisease AT mfenclova bacterialandfungalendophytecommunitiesinhealthyanddiseasedoilseedrapeandtheirpotentialforbiocontrolofsclerotiniaandphomadisease AT kdemnerova bacterialandfungalendophytecommunitiesinhealthyanddiseasedoilseedrapeandtheirpotentialforbiocontrolofsclerotiniaandphomadisease AT mvosatka bacterialandfungalendophytecommunitiesinhealthyanddiseasedoilseedrapeandtheirpotentialforbiocontrolofsclerotiniaandphomadisease
_version_	1718394570451976192

Bacterial and fungal endophyte communities in healthy and diseased oilseed rape and their potential for biocontrol of Sclerotinia and Phoma disease

Ejemplares similares