A split protease-E. coli ClpXP system quantifies protein–protein interactions in Escherichia coli cells

Wang et al. developed a fluorescence-assisted single-cell methodology (split protease-E. coli ClpXP (SPEC)) to characterise protein-protein interactions for both eukaryotic and prokaryotic species in E. coli cells. Their method can quantify these interactions with high sensitivity, easy manipulation...

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Autores principales:	Shengchen Wang, Faying Zhang, Meng Mei, Ting Wang, Yueli Yun, Shihui Yang, Guimin Zhang, Li Yi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/475f0a3ec6e54d2ca2b968a008ba7339
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

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spelling	oai:doaj.org-article:475f0a3ec6e54d2ca2b968a008ba73392021-12-02T15:22:55ZA split protease-E. coli ClpXP system quantifies protein–protein interactions in Escherichia coli cells10.1038/s42003-021-02374-w2399-3642https://doaj.org/article/475f0a3ec6e54d2ca2b968a008ba73392021-07-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02374-whttps://doaj.org/toc/2399-3642Wang et al. developed a fluorescence-assisted single-cell methodology (split protease-E. coli ClpXP (SPEC)) to characterise protein-protein interactions for both eukaryotic and prokaryotic species in E. coli cells. Their method can quantify these interactions with high sensitivity, easy manipulation, and broad temperature adaptabilityShengchen WangFaying ZhangMeng MeiTing WangYueli YunShihui YangGuimin ZhangLi YiNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Shengchen Wang Faying Zhang Meng Mei Ting Wang Yueli Yun Shihui Yang Guimin Zhang Li Yi A split protease-E. coli ClpXP system quantifies protein–protein interactions in Escherichia coli cells
description	Wang et al. developed a fluorescence-assisted single-cell methodology (split protease-E. coli ClpXP (SPEC)) to characterise protein-protein interactions for both eukaryotic and prokaryotic species in E. coli cells. Their method can quantify these interactions with high sensitivity, easy manipulation, and broad temperature adaptability
format	article
author	Shengchen Wang Faying Zhang Meng Mei Ting Wang Yueli Yun Shihui Yang Guimin Zhang Li Yi
author_facet	Shengchen Wang Faying Zhang Meng Mei Ting Wang Yueli Yun Shihui Yang Guimin Zhang Li Yi
author_sort	Shengchen Wang
title	A split protease-E. coli ClpXP system quantifies protein–protein interactions in Escherichia coli cells
title_short	A split protease-E. coli ClpXP system quantifies protein–protein interactions in Escherichia coli cells
title_full	A split protease-E. coli ClpXP system quantifies protein–protein interactions in Escherichia coli cells
title_fullStr	A split protease-E. coli ClpXP system quantifies protein–protein interactions in Escherichia coli cells
title_full_unstemmed	A split protease-E. coli ClpXP system quantifies protein–protein interactions in Escherichia coli cells
title_sort	split protease-e. coli clpxp system quantifies protein–protein interactions in escherichia coli cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/475f0a3ec6e54d2ca2b968a008ba7339
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A split protease-E. coli ClpXP system quantifies protein–protein interactions in Escherichia coli cells

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