Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences.

<h4>Background</h4>CTX-M-producing Escherichia coli strains are regarded as major global pathogens.<h4>Methodology/principal findings</h4>The nucleotide sequence of three plasmids (pEC_B24: 73801-bp; pEC_L8: 118525-bp and pEC_L46: 144871-bp) from Escherichia coli isolates obt...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Annemieke Smet, Filip Van Nieuwerburgh, Tom T M Vandekerckhove, An Martel, Dieter Deforce, Patrick Butaye, Freddy Haesebrouck
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/475fd42426c64748ba37f8bda423cb1a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:475fd42426c64748ba37f8bda423cb1a
record_format	dspace
spelling	oai:doaj.org-article:475fd42426c64748ba37f8bda423cb1a2021-12-02T20:20:41ZComplete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences.1932-620310.1371/journal.pone.0011202https://doaj.org/article/475fd42426c64748ba37f8bda423cb1a2010-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20585456/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>CTX-M-producing Escherichia coli strains are regarded as major global pathogens.<h4>Methodology/principal findings</h4>The nucleotide sequence of three plasmids (pEC_B24: 73801-bp; pEC_L8: 118525-bp and pEC_L46: 144871-bp) from Escherichia coli isolates obtained from patients with urinary tract infections and one plasmid (pEC_Bactec: 92970-bp) from an Escherichia coli strain isolated from the joint of a horse with arthritis were determined. Plasmid pEC_Bactec belongs to the IncI1 group and carries two resistance genes: bla(TEM-1) and bla(CTX-M-15). It shares more than 90% homology with a previously published bla(CTX-M)-plasmid from E. coli of human origin. Plasmid pEC_B24 belongs to the IncFII group whereas plasmids pEC_L8 and pEC_L46 represent a fusion of two replicons of type FII and FIA. On the pEC_B24 backbone, two resistance genes, bla(TEM-1) and bla(CTX-M-15), were found. Six resistance genes, bla(TEM-1), bla(CTX-M-15), bla(OXA-1), aac6'-lb-cr, tetA and catB4, were detected on the pEC_L8 backbone. The same antimicrobial drug resistance genes, with the exception of tetA, were also identified on the pEC_L46 backbone. Genome analysis of all 4 plasmids studied provides evidence of a seemingly frequent transposition event of the bla(CTX-M-15)-ISEcp1 element. This element seems to have a preferred insertion site at the tnpA gene of a bla(TEM)-carrying Tn3-like transposon, the latter itself being inserted by a transposition event. The IS26-composite transposon, which contains the bla(OXA-1), aac6'-lb-cr and catB4 genes, was inserted into plasmids pEC_L8 and pEC_L46 by homologous recombination rather than a transposition event. Results obtained for pEC_L46 indicated that IS26 also plays an important role in structural rearrangements of the plasmid backbone and seems to facilitate the mobilisation of fragments from other plasmids.<h4>Conclusions</h4>Collectively, these data suggests that IS26 together with ISEcp1 could play a critical role in the evolution of diverse multiresistant plasmids found in clinical Enterobacteriaceae.Annemieke SmetFilip Van NieuwerburghTom T M VandekerckhoveAn MartelDieter DeforcePatrick ButayeFreddy HaesebrouckPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 6, p e11202 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Annemieke Smet Filip Van Nieuwerburgh Tom T M Vandekerckhove An Martel Dieter Deforce Patrick Butaye Freddy Haesebrouck Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences.
description	<h4>Background</h4>CTX-M-producing Escherichia coli strains are regarded as major global pathogens.<h4>Methodology/principal findings</h4>The nucleotide sequence of three plasmids (pEC_B24: 73801-bp; pEC_L8: 118525-bp and pEC_L46: 144871-bp) from Escherichia coli isolates obtained from patients with urinary tract infections and one plasmid (pEC_Bactec: 92970-bp) from an Escherichia coli strain isolated from the joint of a horse with arthritis were determined. Plasmid pEC_Bactec belongs to the IncI1 group and carries two resistance genes: bla(TEM-1) and bla(CTX-M-15). It shares more than 90% homology with a previously published bla(CTX-M)-plasmid from E. coli of human origin. Plasmid pEC_B24 belongs to the IncFII group whereas plasmids pEC_L8 and pEC_L46 represent a fusion of two replicons of type FII and FIA. On the pEC_B24 backbone, two resistance genes, bla(TEM-1) and bla(CTX-M-15), were found. Six resistance genes, bla(TEM-1), bla(CTX-M-15), bla(OXA-1), aac6'-lb-cr, tetA and catB4, were detected on the pEC_L8 backbone. The same antimicrobial drug resistance genes, with the exception of tetA, were also identified on the pEC_L46 backbone. Genome analysis of all 4 plasmids studied provides evidence of a seemingly frequent transposition event of the bla(CTX-M-15)-ISEcp1 element. This element seems to have a preferred insertion site at the tnpA gene of a bla(TEM)-carrying Tn3-like transposon, the latter itself being inserted by a transposition event. The IS26-composite transposon, which contains the bla(OXA-1), aac6'-lb-cr and catB4 genes, was inserted into plasmids pEC_L8 and pEC_L46 by homologous recombination rather than a transposition event. Results obtained for pEC_L46 indicated that IS26 also plays an important role in structural rearrangements of the plasmid backbone and seems to facilitate the mobilisation of fragments from other plasmids.<h4>Conclusions</h4>Collectively, these data suggests that IS26 together with ISEcp1 could play a critical role in the evolution of diverse multiresistant plasmids found in clinical Enterobacteriaceae.
format	article
author	Annemieke Smet Filip Van Nieuwerburgh Tom T M Vandekerckhove An Martel Dieter Deforce Patrick Butaye Freddy Haesebrouck
author_facet	Annemieke Smet Filip Van Nieuwerburgh Tom T M Vandekerckhove An Martel Dieter Deforce Patrick Butaye Freddy Haesebrouck
author_sort	Annemieke Smet
title	Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences.
title_short	Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences.
title_full	Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences.
title_fullStr	Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences.
title_full_unstemmed	Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences.
title_sort	complete nucleotide sequence of ctx-m-15-plasmids from clinical escherichia coli isolates: insertional events of transposons and insertion sequences.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/475fd42426c64748ba37f8bda423cb1a
work_keys_str_mv	AT annemiekesmet completenucleotidesequenceofctxm15plasmidsfromclinicalescherichiacoliisolatesinsertionaleventsoftransposonsandinsertionsequences AT filipvannieuwerburgh completenucleotidesequenceofctxm15plasmidsfromclinicalescherichiacoliisolatesinsertionaleventsoftransposonsandinsertionsequences AT tomtmvandekerckhove completenucleotidesequenceofctxm15plasmidsfromclinicalescherichiacoliisolatesinsertionaleventsoftransposonsandinsertionsequences AT anmartel completenucleotidesequenceofctxm15plasmidsfromclinicalescherichiacoliisolatesinsertionaleventsoftransposonsandinsertionsequences AT dieterdeforce completenucleotidesequenceofctxm15plasmidsfromclinicalescherichiacoliisolatesinsertionaleventsoftransposonsandinsertionsequences AT patrickbutaye completenucleotidesequenceofctxm15plasmidsfromclinicalescherichiacoliisolatesinsertionaleventsoftransposonsandinsertionsequences AT freddyhaesebrouck completenucleotidesequenceofctxm15plasmidsfromclinicalescherichiacoliisolatesinsertionaleventsoftransposonsandinsertionsequences
_version_	1718374162899140608

Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences.

Ejemplares similares