Functional and histologic imaging of urinary bladder wall after exposure to psychological stress and protamine sulfate

Abstract To quantify the urinary bladder wall T1 relaxation time (T1) before and after the instillation contrast mixture in rats previously subjected to water avoidance stress (WAS) and/or acute exposure to protamine sulfate (PS). Female Wistar rats were randomized to receive either sham (control) o...

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Autores principales:	Tetsuichi Saito, T. Kevin Hitchens, Lesley M. Foley, Nishant Singh, Shinsuke Mizoguchi, Masahiro Kurobe, Daisuke Gotoh, Teruyuki Ogawa, Tomonori Minagawa, Osamu Ishizuka, Christopher Chermansky, Jonathan Kaufman, Naoki Yoshimura, Pradeep Tyagi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4760258bb244492d84bb423083d095b7
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spelling	oai:doaj.org-article:4760258bb244492d84bb423083d095b72021-12-02T17:37:23ZFunctional and histologic imaging of urinary bladder wall after exposure to psychological stress and protamine sulfate10.1038/s41598-021-98504-92045-2322https://doaj.org/article/4760258bb244492d84bb423083d095b72021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98504-9https://doaj.org/toc/2045-2322Abstract To quantify the urinary bladder wall T1 relaxation time (T1) before and after the instillation contrast mixture in rats previously subjected to water avoidance stress (WAS) and/or acute exposure to protamine sulfate (PS). Female Wistar rats were randomized to receive either sham (control) or 1 h of WAS for ten consecutive days before the evaluation of nocturnal urination pattern in metabolic cages. T1 mapping of urinary bladder wall at 9.4 T was performed pre- and post- instillation of 4 mM Gadobutrol in a mixture with 5 mM Ferumoxytol. Subsequently, either T1 mapping was repeated after brief intravesical PS exposure or the animals were sacrificed for histology and analyzing the mucosal levels of mRNA. Compared to the control group, WAS exposure decreased the single void urine volume and shortened the post-contrast T1 relaxation time of mucosa- used to compute relatively higher ingress of instilled Gadobutrol. Compromised permeability in WAS group was corroborated by the urothelial denudation, edema and ZO-1 downregulation. PS exposure doubled the baseline ingress of Gadobutrol in both groups. These findings confirm that psychological stress compromises the paracellular permeability of bladder mucosa and its non-invasive assay with MRI was validated by PS exposure.Tetsuichi SaitoT. Kevin HitchensLesley M. FoleyNishant SinghShinsuke MizoguchiMasahiro KurobeDaisuke GotohTeruyuki OgawaTomonori MinagawaOsamu IshizukaChristopher ChermanskyJonathan KaufmanNaoki YoshimuraPradeep TyagiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tetsuichi Saito T. Kevin Hitchens Lesley M. Foley Nishant Singh Shinsuke Mizoguchi Masahiro Kurobe Daisuke Gotoh Teruyuki Ogawa Tomonori Minagawa Osamu Ishizuka Christopher Chermansky Jonathan Kaufman Naoki Yoshimura Pradeep Tyagi Functional and histologic imaging of urinary bladder wall after exposure to psychological stress and protamine sulfate
description	Abstract To quantify the urinary bladder wall T1 relaxation time (T1) before and after the instillation contrast mixture in rats previously subjected to water avoidance stress (WAS) and/or acute exposure to protamine sulfate (PS). Female Wistar rats were randomized to receive either sham (control) or 1 h of WAS for ten consecutive days before the evaluation of nocturnal urination pattern in metabolic cages. T1 mapping of urinary bladder wall at 9.4 T was performed pre- and post- instillation of 4 mM Gadobutrol in a mixture with 5 mM Ferumoxytol. Subsequently, either T1 mapping was repeated after brief intravesical PS exposure or the animals were sacrificed for histology and analyzing the mucosal levels of mRNA. Compared to the control group, WAS exposure decreased the single void urine volume and shortened the post-contrast T1 relaxation time of mucosa- used to compute relatively higher ingress of instilled Gadobutrol. Compromised permeability in WAS group was corroborated by the urothelial denudation, edema and ZO-1 downregulation. PS exposure doubled the baseline ingress of Gadobutrol in both groups. These findings confirm that psychological stress compromises the paracellular permeability of bladder mucosa and its non-invasive assay with MRI was validated by PS exposure.
format	article
author	Tetsuichi Saito T. Kevin Hitchens Lesley M. Foley Nishant Singh Shinsuke Mizoguchi Masahiro Kurobe Daisuke Gotoh Teruyuki Ogawa Tomonori Minagawa Osamu Ishizuka Christopher Chermansky Jonathan Kaufman Naoki Yoshimura Pradeep Tyagi
author_facet	Tetsuichi Saito T. Kevin Hitchens Lesley M. Foley Nishant Singh Shinsuke Mizoguchi Masahiro Kurobe Daisuke Gotoh Teruyuki Ogawa Tomonori Minagawa Osamu Ishizuka Christopher Chermansky Jonathan Kaufman Naoki Yoshimura Pradeep Tyagi
author_sort	Tetsuichi Saito
title	Functional and histologic imaging of urinary bladder wall after exposure to psychological stress and protamine sulfate
title_short	Functional and histologic imaging of urinary bladder wall after exposure to psychological stress and protamine sulfate
title_full	Functional and histologic imaging of urinary bladder wall after exposure to psychological stress and protamine sulfate
title_fullStr	Functional and histologic imaging of urinary bladder wall after exposure to psychological stress and protamine sulfate
title_full_unstemmed	Functional and histologic imaging of urinary bladder wall after exposure to psychological stress and protamine sulfate
title_sort	functional and histologic imaging of urinary bladder wall after exposure to psychological stress and protamine sulfate
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4760258bb244492d84bb423083d095b7
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Functional and histologic imaging of urinary bladder wall after exposure to psychological stress and protamine sulfate

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