Emerging MDR-Pseudomonas aeruginosa in fish commonly harbor oprL and toxA virulence genes and bla TEM, bla CTX-M, and tetA antibiotic-resistance genes

Abstract This study aimed to investigate the prevalence, antibiogram of Pseudomonas aeruginosa (P. aeruginosa), and the distribution of virulence genes (oprL, exoS, phzM, and toxA) and the antibiotic-resistance genes (bla TEM, tetA, and bla CTX-M). A total of 285 fish (165 Oreochromis niloticus and...

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Autores principales:	Abdelazeem M. Algammal, Mahmoud Mabrok, Elayaraja Sivaramasamy, Fatma M. Youssef, Mona H. Atwa, Ali W. El-kholy, Helal F. Hetta, Wael N. Hozzein
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/476297176b4c4bfbbaddd8e35cb51d62
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spelling	oai:doaj.org-article:476297176b4c4bfbbaddd8e35cb51d622021-12-02T18:51:27ZEmerging MDR-Pseudomonas aeruginosa in fish commonly harbor oprL and toxA virulence genes and bla TEM, bla CTX-M, and tetA antibiotic-resistance genes10.1038/s41598-020-72264-42045-2322https://doaj.org/article/476297176b4c4bfbbaddd8e35cb51d622020-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-72264-4https://doaj.org/toc/2045-2322Abstract This study aimed to investigate the prevalence, antibiogram of Pseudomonas aeruginosa (P. aeruginosa), and the distribution of virulence genes (oprL, exoS, phzM, and toxA) and the antibiotic-resistance genes (bla TEM, tetA, and bla CTX-M). A total of 285 fish (165 Oreochromis niloticus and 120 Clarias gariepinus) were collected randomly from private fish farms in Ismailia Governorate, Egypt. The collected specimens were examined bacteriologically. P. aeruginosa was isolated from 90 examined fish (31.57%), and the liver was the most prominent infected organ. The antibiogram of the isolated strains was determined using a disc diffusion method, where the tested strains exhibited multi-drug resistance (MDR) to amoxicillin, cefotaxime, tetracycline, and gentamicin. The PCR results revealed that all the examined strains harbored (oprL and toxA) virulence genes, while only 22.2% were positive for the phzM gene. On the contrary, none of the tested strains were positive for the exoS gene. Concerning the distribution of the antibiotic resistance genes, the examined strains harbored bla TEM, bla CTX-M , and tetA genes with a total prevalence of 83.3%, 77.7%, and 75.6%, respectively. Experimentally infected fish with P. aeruginosa displayed high mortalities in direct proportion to the encoded virulence genes and showed similar signs of septicemia found in the naturally infected one. In conclusion, P. aeruginosa is a major pathogen of O. niloticus and C. gariepinus. oprL and toxA genes are the most predominant virulence genes associated with P. aeruginosa infection. The bla CTX-M , bla TEM, and tetA genes are the main antibiotic-resistance genes that induce resistance patterns to cefotaxime, amoxicillin, and tetracycline, highlighting MDR P. aeruginosa strains of potential public health concern.Abdelazeem M. AlgammalMahmoud MabrokElayaraja SivaramasamyFatma M. YoussefMona H. AtwaAli W. El-kholyHelal F. HettaWael N. HozzeinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Abdelazeem M. Algammal Mahmoud Mabrok Elayaraja Sivaramasamy Fatma M. Youssef Mona H. Atwa Ali W. El-kholy Helal F. Hetta Wael N. Hozzein Emerging MDR-Pseudomonas aeruginosa in fish commonly harbor oprL and toxA virulence genes and bla TEM, bla CTX-M, and tetA antibiotic-resistance genes
description	Abstract This study aimed to investigate the prevalence, antibiogram of Pseudomonas aeruginosa (P. aeruginosa), and the distribution of virulence genes (oprL, exoS, phzM, and toxA) and the antibiotic-resistance genes (bla TEM, tetA, and bla CTX-M). A total of 285 fish (165 Oreochromis niloticus and 120 Clarias gariepinus) were collected randomly from private fish farms in Ismailia Governorate, Egypt. The collected specimens were examined bacteriologically. P. aeruginosa was isolated from 90 examined fish (31.57%), and the liver was the most prominent infected organ. The antibiogram of the isolated strains was determined using a disc diffusion method, where the tested strains exhibited multi-drug resistance (MDR) to amoxicillin, cefotaxime, tetracycline, and gentamicin. The PCR results revealed that all the examined strains harbored (oprL and toxA) virulence genes, while only 22.2% were positive for the phzM gene. On the contrary, none of the tested strains were positive for the exoS gene. Concerning the distribution of the antibiotic resistance genes, the examined strains harbored bla TEM, bla CTX-M , and tetA genes with a total prevalence of 83.3%, 77.7%, and 75.6%, respectively. Experimentally infected fish with P. aeruginosa displayed high mortalities in direct proportion to the encoded virulence genes and showed similar signs of septicemia found in the naturally infected one. In conclusion, P. aeruginosa is a major pathogen of O. niloticus and C. gariepinus. oprL and toxA genes are the most predominant virulence genes associated with P. aeruginosa infection. The bla CTX-M , bla TEM, and tetA genes are the main antibiotic-resistance genes that induce resistance patterns to cefotaxime, amoxicillin, and tetracycline, highlighting MDR P. aeruginosa strains of potential public health concern.
format	article
author	Abdelazeem M. Algammal Mahmoud Mabrok Elayaraja Sivaramasamy Fatma M. Youssef Mona H. Atwa Ali W. El-kholy Helal F. Hetta Wael N. Hozzein
author_facet	Abdelazeem M. Algammal Mahmoud Mabrok Elayaraja Sivaramasamy Fatma M. Youssef Mona H. Atwa Ali W. El-kholy Helal F. Hetta Wael N. Hozzein
author_sort	Abdelazeem M. Algammal
title	Emerging MDR-Pseudomonas aeruginosa in fish commonly harbor oprL and toxA virulence genes and bla TEM, bla CTX-M, and tetA antibiotic-resistance genes
title_short	Emerging MDR-Pseudomonas aeruginosa in fish commonly harbor oprL and toxA virulence genes and bla TEM, bla CTX-M, and tetA antibiotic-resistance genes
title_full	Emerging MDR-Pseudomonas aeruginosa in fish commonly harbor oprL and toxA virulence genes and bla TEM, bla CTX-M, and tetA antibiotic-resistance genes
title_fullStr	Emerging MDR-Pseudomonas aeruginosa in fish commonly harbor oprL and toxA virulence genes and bla TEM, bla CTX-M, and tetA antibiotic-resistance genes
title_full_unstemmed	Emerging MDR-Pseudomonas aeruginosa in fish commonly harbor oprL and toxA virulence genes and bla TEM, bla CTX-M, and tetA antibiotic-resistance genes
title_sort	emerging mdr-pseudomonas aeruginosa in fish commonly harbor oprl and toxa virulence genes and bla tem, bla ctx-m, and teta antibiotic-resistance genes
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/476297176b4c4bfbbaddd8e35cb51d62
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Emerging MDR-Pseudomonas aeruginosa in fish commonly harbor oprL and toxA virulence genes and bla TEM, bla CTX-M, and tetA antibiotic-resistance genes

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