L-Tyrosine-loaded nanoparticles increase the antitumoral activity of direct electric current in a metastatic melanoma cell model

L-Tyrosine-loaded nanoparticles increase the antitumoral activity of direct electric current in a metastatic melanoma cell model

Vânia Emerich Bucco de Campos1, Cesar Augusto Antunes Teixeira1, Venicio Feo da Veiga2, Eduardo Ricci Júnior1, Carla Holandino11Departamento de Medicamentos, Faculdade de Farmácia, 2Instituto de Microbiologia Professor Paulo de Góes, Univers...

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Autores principales: Vânia Emerich Bucco de Campos, Cesar Augusto Antunes Teixeira, Venicio Feo da Veiga, et al
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2010
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/4773385aa02444639610d634b3849a36
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spelling oai:doaj.org-article:4773385aa02444639610d634b3849a362021-12-02T04:21:21ZL-Tyrosine-loaded nanoparticles increase the antitumoral activity of direct electric current in a metastatic melanoma cell model1176-91141178-2013https://doaj.org/article/4773385aa02444639610d634b3849a362010-11-01T00:00:00Zhttp://www.dovepress.com/l-tyrosine-loaded-nanoparticles-increase-the-antitumoral-activity-of-d-a5645https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Vânia Emerich Bucco de Campos1, Cesar Augusto Antunes Teixeira1, Venicio Feo da Veiga2, Eduardo Ricci Júnior1, Carla Holandino11Departamento de Medicamentos, Faculdade de Farmácia, 2Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilAbstract: Inhibition of tumor growth induced by treatment with direct electric current (DC) has been reported in several models. One of the mechanisms responsible for the antitumoral activity of DC is the generation of oxidative species, known as chloramines. With the aim of increasing chloramine production in the electrolytic medium and optimizing the antitumoral effects of DC, poly(e-caprolactone) (PCL) nanoparticles (NPs) loaded with the amino acid tyrosine were obtained. The physical–chemical characterization showed that the NPs presented size in nanometric range and monomodal distribution. A slightly negative electrokinetic potential was also found in both blank NPs and L-tyrosine-loaded PCL NPs. The yield of the loading process was approximately 50%. Within 3 h of dissolution assay, a burst release of about 80% L-tyrosine was obtained. The in vitro cytotoxicity of DC was significantly increased when associated with L-tyrosine-loaded NPs, using a murine multidrug-resistant melanoma cell line model. This study showed that the use of the combination of nanotechnology and DC has a promising antineoplastic potential and opens a new perspective in cancer therapy.Keywords: direct electric current, nanotechnology, cancer therapy, L-tyrosine, B16F10 cells Vânia Emerich Bucco de CamposCesar Augusto Antunes TeixeiraVenicio Feo da Veigaet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 961-971 (2010)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Vânia Emerich Bucco de Campos
Cesar Augusto Antunes Teixeira
Venicio Feo da Veiga
et al
L-Tyrosine-loaded nanoparticles increase the antitumoral activity of direct electric current in a metastatic melanoma cell model
description Vânia Emerich Bucco de Campos1, Cesar Augusto Antunes Teixeira1, Venicio Feo da Veiga2, Eduardo Ricci Júnior1, Carla Holandino11Departamento de Medicamentos, Faculdade de Farmácia, 2Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilAbstract: Inhibition of tumor growth induced by treatment with direct electric current (DC) has been reported in several models. One of the mechanisms responsible for the antitumoral activity of DC is the generation of oxidative species, known as chloramines. With the aim of increasing chloramine production in the electrolytic medium and optimizing the antitumoral effects of DC, poly(e-caprolactone) (PCL) nanoparticles (NPs) loaded with the amino acid tyrosine were obtained. The physical–chemical characterization showed that the NPs presented size in nanometric range and monomodal distribution. A slightly negative electrokinetic potential was also found in both blank NPs and L-tyrosine-loaded PCL NPs. The yield of the loading process was approximately 50%. Within 3 h of dissolution assay, a burst release of about 80% L-tyrosine was obtained. The in vitro cytotoxicity of DC was significantly increased when associated with L-tyrosine-loaded NPs, using a murine multidrug-resistant melanoma cell line model. This study showed that the use of the combination of nanotechnology and DC has a promising antineoplastic potential and opens a new perspective in cancer therapy.Keywords: direct electric current, nanotechnology, cancer therapy, L-tyrosine, B16F10 cells
format article
author Vânia Emerich Bucco de Campos
Cesar Augusto Antunes Teixeira
Venicio Feo da Veiga
et al
author_facet Vânia Emerich Bucco de Campos
Cesar Augusto Antunes Teixeira
Venicio Feo da Veiga
et al
author_sort Vânia Emerich Bucco de Campos
title L-Tyrosine-loaded nanoparticles increase the antitumoral activity of direct electric current in a metastatic melanoma cell model
title_short L-Tyrosine-loaded nanoparticles increase the antitumoral activity of direct electric current in a metastatic melanoma cell model
title_full L-Tyrosine-loaded nanoparticles increase the antitumoral activity of direct electric current in a metastatic melanoma cell model
title_fullStr L-Tyrosine-loaded nanoparticles increase the antitumoral activity of direct electric current in a metastatic melanoma cell model
title_full_unstemmed L-Tyrosine-loaded nanoparticles increase the antitumoral activity of direct electric current in a metastatic melanoma cell model
title_sort l-tyrosine-loaded nanoparticles increase the antitumoral activity of direct electric current in a metastatic melanoma cell model
publisher Dove Medical Press
publishDate 2010
url https://doaj.org/article/4773385aa02444639610d634b3849a36
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