eDNA metabarcoding for biodiversity assessment, generalist predators as sampling assistants

Abstract With an accelerating negative impact of anthropogenic actions on natural ecosystems, non-invasive biodiversity assessments are becoming increasingly crucial. As a consequence, the interest in the application of environmental DNA (eDNA) survey techniques has increased. The use of eDNA extrac...

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Autores principales:	Louise Nørgaard, Carsten Riis Olesen, Kristian Trøjelsgaard, Cino Pertoldi, Jeppe Lund Nielsen, Pierre Taberlet, Aritz Ruiz-González, Marta De Barba, Laura Iacolina
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4774e761e7bc46e2820a17bc2728a210
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spelling	oai:doaj.org-article:4774e761e7bc46e2820a17bc2728a2102021-12-02T16:35:56ZeDNA metabarcoding for biodiversity assessment, generalist predators as sampling assistants10.1038/s41598-021-85488-92045-2322https://doaj.org/article/4774e761e7bc46e2820a17bc2728a2102021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85488-9https://doaj.org/toc/2045-2322Abstract With an accelerating negative impact of anthropogenic actions on natural ecosystems, non-invasive biodiversity assessments are becoming increasingly crucial. As a consequence, the interest in the application of environmental DNA (eDNA) survey techniques has increased. The use of eDNA extracted from faeces from generalist predators, have recently been described as “biodiversity capsules” and suggested as a complementary tool for improving current biodiversity assessments. In this study, using faecal samples from two generalist omnivore species, the Eurasian badger and the red fox, we evaluated the applicability of eDNA metabarcoding in determining dietary composition, compared to macroscopic diet identification techniques. Subsequently, we used the dietary information obtained to assess its contribution to biodiversity assessments. Compared to classic macroscopic techniques, we found that eDNA metabarcoding detected more taxa, at higher taxonomic resolution, and proved to be an important technique to verify the species identification of the predator from field collected faeces. Furthermore, we showed how dietary analyses complemented field observations in describing biodiversity by identifying consumed flora and fauna that went unnoticed during field observations. While diet analysis approaches could not substitute field observations entirely, we suggest that their integration with other methods might overcome intrinsic limitations of single techniques in future biodiversity surveys.Louise NørgaardCarsten Riis OlesenKristian TrøjelsgaardCino PertoldiJeppe Lund NielsenPierre TaberletAritz Ruiz-GonzálezMarta De BarbaLaura IacolinaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Louise Nørgaard Carsten Riis Olesen Kristian Trøjelsgaard Cino Pertoldi Jeppe Lund Nielsen Pierre Taberlet Aritz Ruiz-González Marta De Barba Laura Iacolina eDNA metabarcoding for biodiversity assessment, generalist predators as sampling assistants
description	Abstract With an accelerating negative impact of anthropogenic actions on natural ecosystems, non-invasive biodiversity assessments are becoming increasingly crucial. As a consequence, the interest in the application of environmental DNA (eDNA) survey techniques has increased. The use of eDNA extracted from faeces from generalist predators, have recently been described as “biodiversity capsules” and suggested as a complementary tool for improving current biodiversity assessments. In this study, using faecal samples from two generalist omnivore species, the Eurasian badger and the red fox, we evaluated the applicability of eDNA metabarcoding in determining dietary composition, compared to macroscopic diet identification techniques. Subsequently, we used the dietary information obtained to assess its contribution to biodiversity assessments. Compared to classic macroscopic techniques, we found that eDNA metabarcoding detected more taxa, at higher taxonomic resolution, and proved to be an important technique to verify the species identification of the predator from field collected faeces. Furthermore, we showed how dietary analyses complemented field observations in describing biodiversity by identifying consumed flora and fauna that went unnoticed during field observations. While diet analysis approaches could not substitute field observations entirely, we suggest that their integration with other methods might overcome intrinsic limitations of single techniques in future biodiversity surveys.
format	article
author	Louise Nørgaard Carsten Riis Olesen Kristian Trøjelsgaard Cino Pertoldi Jeppe Lund Nielsen Pierre Taberlet Aritz Ruiz-González Marta De Barba Laura Iacolina
author_facet	Louise Nørgaard Carsten Riis Olesen Kristian Trøjelsgaard Cino Pertoldi Jeppe Lund Nielsen Pierre Taberlet Aritz Ruiz-González Marta De Barba Laura Iacolina
author_sort	Louise Nørgaard
title	eDNA metabarcoding for biodiversity assessment, generalist predators as sampling assistants
title_short	eDNA metabarcoding for biodiversity assessment, generalist predators as sampling assistants
title_full	eDNA metabarcoding for biodiversity assessment, generalist predators as sampling assistants
title_fullStr	eDNA metabarcoding for biodiversity assessment, generalist predators as sampling assistants
title_full_unstemmed	eDNA metabarcoding for biodiversity assessment, generalist predators as sampling assistants
title_sort	edna metabarcoding for biodiversity assessment, generalist predators as sampling assistants
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4774e761e7bc46e2820a17bc2728a210
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eDNA metabarcoding for biodiversity assessment, generalist predators as sampling assistants

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