Increased urinary angiotensin-converting enzyme 2 in renal transplant patients with diabetes.

Angiotensin-converting enzyme 2 (ACE2) is expressed in the kidney and may be a renoprotective enzyme, since it converts angiotensin (Ang) II to Ang-(1-7). ACE2 has been detected in urine from patients with chronic kidney disease. We measured urinary ACE2 activity and protein levels in renal transpla...

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Autores principales:	Fengxia Xiao, Swapnil Hiremath, Greg Knoll, Joseph Zimpelmann, Kajenny Srivaratharajah, Deepak Jadhav, Dean Fergusson, Chris R J Kennedy, Kevin D Burns
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/477579ad14544d03a13fcae62e1094a6
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spelling	oai:doaj.org-article:477579ad14544d03a13fcae62e1094a62021-11-18T07:17:48ZIncreased urinary angiotensin-converting enzyme 2 in renal transplant patients with diabetes.1932-620310.1371/journal.pone.0037649https://doaj.org/article/477579ad14544d03a13fcae62e1094a62012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22629438/?tool=EBIhttps://doaj.org/toc/1932-6203Angiotensin-converting enzyme 2 (ACE2) is expressed in the kidney and may be a renoprotective enzyme, since it converts angiotensin (Ang) II to Ang-(1-7). ACE2 has been detected in urine from patients with chronic kidney disease. We measured urinary ACE2 activity and protein levels in renal transplant patients (age 54 yrs, 65% male, 38% diabetes, n = 100) and healthy controls (age 45 yrs, 26% male, n = 50), and determined factors associated with elevated urinary ACE2 in the patients. Urine from transplant subjects was also assayed for ACE mRNA and protein. No subjects were taking inhibitors of the renin-angiotensin system. Urinary ACE2 levels were significantly higher in transplant patients compared to controls (p = 0.003 for ACE2 activity, and p≤0.001 for ACE2 protein by ELISA or western analysis). Transplant patients with diabetes mellitus had significantly increased urinary ACE2 activity and protein levels compared to non-diabetics (p<0.001), while ACE2 mRNA levels did not differ. Urinary ACE activity and protein were significantly increased in diabetic transplant subjects, while ACE mRNA levels did not differ from non-diabetic subjects. After adjusting for confounding variables, diabetes was significantly associated with urinary ACE2 activity (p = 0.003) and protein levels (p<0.001), while female gender was associated with urinary mRNA levels for both ACE2 and ACE. These data indicate that urinary ACE2 is increased in renal transplant recipients with diabetes, possibly due to increased shedding from tubular cells. Urinary ACE2 could be a marker of renal renin-angiotensin system activation in these patients.Fengxia XiaoSwapnil HiremathGreg KnollJoseph ZimpelmannKajenny SrivaratharajahDeepak JadhavDean FergussonChris R J KennedyKevin D BurnsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e37649 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fengxia Xiao Swapnil Hiremath Greg Knoll Joseph Zimpelmann Kajenny Srivaratharajah Deepak Jadhav Dean Fergusson Chris R J Kennedy Kevin D Burns Increased urinary angiotensin-converting enzyme 2 in renal transplant patients with diabetes.
description	Angiotensin-converting enzyme 2 (ACE2) is expressed in the kidney and may be a renoprotective enzyme, since it converts angiotensin (Ang) II to Ang-(1-7). ACE2 has been detected in urine from patients with chronic kidney disease. We measured urinary ACE2 activity and protein levels in renal transplant patients (age 54 yrs, 65% male, 38% diabetes, n = 100) and healthy controls (age 45 yrs, 26% male, n = 50), and determined factors associated with elevated urinary ACE2 in the patients. Urine from transplant subjects was also assayed for ACE mRNA and protein. No subjects were taking inhibitors of the renin-angiotensin system. Urinary ACE2 levels were significantly higher in transplant patients compared to controls (p = 0.003 for ACE2 activity, and p≤0.001 for ACE2 protein by ELISA or western analysis). Transplant patients with diabetes mellitus had significantly increased urinary ACE2 activity and protein levels compared to non-diabetics (p<0.001), while ACE2 mRNA levels did not differ. Urinary ACE activity and protein were significantly increased in diabetic transplant subjects, while ACE mRNA levels did not differ from non-diabetic subjects. After adjusting for confounding variables, diabetes was significantly associated with urinary ACE2 activity (p = 0.003) and protein levels (p<0.001), while female gender was associated with urinary mRNA levels for both ACE2 and ACE. These data indicate that urinary ACE2 is increased in renal transplant recipients with diabetes, possibly due to increased shedding from tubular cells. Urinary ACE2 could be a marker of renal renin-angiotensin system activation in these patients.
format	article
author	Fengxia Xiao Swapnil Hiremath Greg Knoll Joseph Zimpelmann Kajenny Srivaratharajah Deepak Jadhav Dean Fergusson Chris R J Kennedy Kevin D Burns
author_facet	Fengxia Xiao Swapnil Hiremath Greg Knoll Joseph Zimpelmann Kajenny Srivaratharajah Deepak Jadhav Dean Fergusson Chris R J Kennedy Kevin D Burns
author_sort	Fengxia Xiao
title	Increased urinary angiotensin-converting enzyme 2 in renal transplant patients with diabetes.
title_short	Increased urinary angiotensin-converting enzyme 2 in renal transplant patients with diabetes.
title_full	Increased urinary angiotensin-converting enzyme 2 in renal transplant patients with diabetes.
title_fullStr	Increased urinary angiotensin-converting enzyme 2 in renal transplant patients with diabetes.
title_full_unstemmed	Increased urinary angiotensin-converting enzyme 2 in renal transplant patients with diabetes.
title_sort	increased urinary angiotensin-converting enzyme 2 in renal transplant patients with diabetes.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/477579ad14544d03a13fcae62e1094a6
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Increased urinary angiotensin-converting enzyme 2 in renal transplant patients with diabetes.

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