Overexpression of CD44 in neural precursor cells improves trans-endothelial migration and facilitates their invasion of perivascular tissues in vivo.

Neural precursor (NPC) based therapies are used to restore neurons or oligodendrocytes and/or provide neuroprotection in a large variety of neurological diseases. In multiple sclerosis models, intravenously (i.v) -delivered NPCs reduced clinical signs via immunomodulation. We demonstrated recently t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cyrille Deboux, Sophia Ladraa, Sylvie Cazaubon, Siham Ghribi-Mallah, Nicolas Weiss, Nathalie Chaverot, Pierre Olivier Couraud, Anne Baron-Van Evercooren
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4778c268598a49f7a24368080af27996
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4778c268598a49f7a24368080af27996
record_format	dspace
spelling	oai:doaj.org-article:4778c268598a49f7a24368080af279962021-11-18T07:55:31ZOverexpression of CD44 in neural precursor cells improves trans-endothelial migration and facilitates their invasion of perivascular tissues in vivo.1932-620310.1371/journal.pone.0057430https://doaj.org/article/4778c268598a49f7a24368080af279962013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23468987/?tool=EBIhttps://doaj.org/toc/1932-6203Neural precursor (NPC) based therapies are used to restore neurons or oligodendrocytes and/or provide neuroprotection in a large variety of neurological diseases. In multiple sclerosis models, intravenously (i.v) -delivered NPCs reduced clinical signs via immunomodulation. We demonstrated recently that NPCs were able to cross cerebral endothelial cells in vitro and that the multifunctional signalling molecule, CD44 involved in trans-endothelial migration of lymphocytes to sites of inflammation, plays a crucial role in extravasation of syngeneic NPCs. In view of the role of CD44 in NPCs trans-endothelial migration in vitro, we questioned presently the benefit of CD44 overexpression by NPCs in vitro and in vivo, in EAE mice. We show that overexpression of CD44 by NPCs enhanced over 2 folds their trans-endothelial migration in vitro, without impinging on the proliferation or differentiation potential of the transduced cells. Moreover, CD44 overexpression by NPCs improved significantly their elongation, spreading and number of filopodia over the extracellular matrix protein laminin in vitro. We then tested the effect of CD44 overexpression after i.v. delivery in the tail vein of EAE mice. CD44 overexpression was functional invivo as it accelerated trans-endothelial migration and facilitated invasion of HA expressing perivascular sites. These in vitro and in vivo data suggest that CD44 may be crucial not only for NPC crossing the endothelial layer but also for facilitating invasion of extravascular tissues.Cyrille DebouxSophia LadraaSylvie CazaubonSiham Ghribi-MallahNicolas WeissNathalie ChaverotPierre Olivier CouraudAnne Baron-Van EvercoorenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e57430 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cyrille Deboux Sophia Ladraa Sylvie Cazaubon Siham Ghribi-Mallah Nicolas Weiss Nathalie Chaverot Pierre Olivier Couraud Anne Baron-Van Evercooren Overexpression of CD44 in neural precursor cells improves trans-endothelial migration and facilitates their invasion of perivascular tissues in vivo.
description	Neural precursor (NPC) based therapies are used to restore neurons or oligodendrocytes and/or provide neuroprotection in a large variety of neurological diseases. In multiple sclerosis models, intravenously (i.v) -delivered NPCs reduced clinical signs via immunomodulation. We demonstrated recently that NPCs were able to cross cerebral endothelial cells in vitro and that the multifunctional signalling molecule, CD44 involved in trans-endothelial migration of lymphocytes to sites of inflammation, plays a crucial role in extravasation of syngeneic NPCs. In view of the role of CD44 in NPCs trans-endothelial migration in vitro, we questioned presently the benefit of CD44 overexpression by NPCs in vitro and in vivo, in EAE mice. We show that overexpression of CD44 by NPCs enhanced over 2 folds their trans-endothelial migration in vitro, without impinging on the proliferation or differentiation potential of the transduced cells. Moreover, CD44 overexpression by NPCs improved significantly their elongation, spreading and number of filopodia over the extracellular matrix protein laminin in vitro. We then tested the effect of CD44 overexpression after i.v. delivery in the tail vein of EAE mice. CD44 overexpression was functional invivo as it accelerated trans-endothelial migration and facilitated invasion of HA expressing perivascular sites. These in vitro and in vivo data suggest that CD44 may be crucial not only for NPC crossing the endothelial layer but also for facilitating invasion of extravascular tissues.
format	article
author	Cyrille Deboux Sophia Ladraa Sylvie Cazaubon Siham Ghribi-Mallah Nicolas Weiss Nathalie Chaverot Pierre Olivier Couraud Anne Baron-Van Evercooren
author_facet	Cyrille Deboux Sophia Ladraa Sylvie Cazaubon Siham Ghribi-Mallah Nicolas Weiss Nathalie Chaverot Pierre Olivier Couraud Anne Baron-Van Evercooren
author_sort	Cyrille Deboux
title	Overexpression of CD44 in neural precursor cells improves trans-endothelial migration and facilitates their invasion of perivascular tissues in vivo.
title_short	Overexpression of CD44 in neural precursor cells improves trans-endothelial migration and facilitates their invasion of perivascular tissues in vivo.
title_full	Overexpression of CD44 in neural precursor cells improves trans-endothelial migration and facilitates their invasion of perivascular tissues in vivo.
title_fullStr	Overexpression of CD44 in neural precursor cells improves trans-endothelial migration and facilitates their invasion of perivascular tissues in vivo.
title_full_unstemmed	Overexpression of CD44 in neural precursor cells improves trans-endothelial migration and facilitates their invasion of perivascular tissues in vivo.
title_sort	overexpression of cd44 in neural precursor cells improves trans-endothelial migration and facilitates their invasion of perivascular tissues in vivo.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/4778c268598a49f7a24368080af27996
work_keys_str_mv	AT cyrilledeboux overexpressionofcd44inneuralprecursorcellsimprovestransendothelialmigrationandfacilitatestheirinvasionofperivasculartissuesinvivo AT sophialadraa overexpressionofcd44inneuralprecursorcellsimprovestransendothelialmigrationandfacilitatestheirinvasionofperivasculartissuesinvivo AT sylviecazaubon overexpressionofcd44inneuralprecursorcellsimprovestransendothelialmigrationandfacilitatestheirinvasionofperivasculartissuesinvivo AT sihamghribimallah overexpressionofcd44inneuralprecursorcellsimprovestransendothelialmigrationandfacilitatestheirinvasionofperivasculartissuesinvivo AT nicolasweiss overexpressionofcd44inneuralprecursorcellsimprovestransendothelialmigrationandfacilitatestheirinvasionofperivasculartissuesinvivo AT nathaliechaverot overexpressionofcd44inneuralprecursorcellsimprovestransendothelialmigrationandfacilitatestheirinvasionofperivasculartissuesinvivo AT pierreoliviercouraud overexpressionofcd44inneuralprecursorcellsimprovestransendothelialmigrationandfacilitatestheirinvasionofperivasculartissuesinvivo AT annebaronvanevercooren overexpressionofcd44inneuralprecursorcellsimprovestransendothelialmigrationandfacilitatestheirinvasionofperivasculartissuesinvivo
_version_	1718422725551194112

Overexpression of CD44 in neural precursor cells improves trans-endothelial migration and facilitates their invasion of perivascular tissues in vivo.

Ejemplares similares