Investigation of genetic markers for intramuscular fat in the hybrid Wagyu cattle with bulked segregant analysis

Abstract ulked Segregant Analysis (BSA) is a rapid strategy for identifying genetic markers in specific regions of the phenotypical population and it has been widely used for QTLs mapping in smaller mixed F2 and F3 populations. We applied a modified BSA method to assessed genome-wide homozygous and...

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Autores principales:	Yun Zhu, Liyun Han, Peng Li, Xiaolong Kang, Xingang Dan, Yun Ma, Yuangang Shi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/477bd8600cbb453aae6d9114349a7093
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spelling	oai:doaj.org-article:477bd8600cbb453aae6d9114349a70932021-12-02T17:50:41ZInvestigation of genetic markers for intramuscular fat in the hybrid Wagyu cattle with bulked segregant analysis10.1038/s41598-021-91101-w2045-2322https://doaj.org/article/477bd8600cbb453aae6d9114349a70932021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91101-whttps://doaj.org/toc/2045-2322Abstract ulked Segregant Analysis (BSA) is a rapid strategy for identifying genetic markers in specific regions of the phenotypical population and it has been widely used for QTLs mapping in smaller mixed F2 and F3 populations. We applied a modified BSA method to assessed genome-wide homozygous and heterozygous linkage patterns in the Chinese Wagyu Beef Cattle F2/F3 mixed population. Two overlapped regions from F2 and F3 populations on autosomes were found with high-density heterozygote alleles between high and low intramuscular fat groups. Regions from 24.8 M ~ 29.6 M of chromosome 23 were identified as most significantly correlated to the intramuscular fat in our samples. We also identified other 4 potential loci on chromosomes 5, 9, 15, and 21 correlated with Intramuscular fat. This study provided a novel low-cost method for QTLs mapping and identify molecular markers of phenotypical changes in a small mixed population.Yun ZhuLiyun HanPeng LiXiaolong KangXingang DanYun MaYuangang ShiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yun Zhu Liyun Han Peng Li Xiaolong Kang Xingang Dan Yun Ma Yuangang Shi Investigation of genetic markers for intramuscular fat in the hybrid Wagyu cattle with bulked segregant analysis
description	Abstract ulked Segregant Analysis (BSA) is a rapid strategy for identifying genetic markers in specific regions of the phenotypical population and it has been widely used for QTLs mapping in smaller mixed F2 and F3 populations. We applied a modified BSA method to assessed genome-wide homozygous and heterozygous linkage patterns in the Chinese Wagyu Beef Cattle F2/F3 mixed population. Two overlapped regions from F2 and F3 populations on autosomes were found with high-density heterozygote alleles between high and low intramuscular fat groups. Regions from 24.8 M ~ 29.6 M of chromosome 23 were identified as most significantly correlated to the intramuscular fat in our samples. We also identified other 4 potential loci on chromosomes 5, 9, 15, and 21 correlated with Intramuscular fat. This study provided a novel low-cost method for QTLs mapping and identify molecular markers of phenotypical changes in a small mixed population.
format	article
author	Yun Zhu Liyun Han Peng Li Xiaolong Kang Xingang Dan Yun Ma Yuangang Shi
author_facet	Yun Zhu Liyun Han Peng Li Xiaolong Kang Xingang Dan Yun Ma Yuangang Shi
author_sort	Yun Zhu
title	Investigation of genetic markers for intramuscular fat in the hybrid Wagyu cattle with bulked segregant analysis
title_short	Investigation of genetic markers for intramuscular fat in the hybrid Wagyu cattle with bulked segregant analysis
title_full	Investigation of genetic markers for intramuscular fat in the hybrid Wagyu cattle with bulked segregant analysis
title_fullStr	Investigation of genetic markers for intramuscular fat in the hybrid Wagyu cattle with bulked segregant analysis
title_full_unstemmed	Investigation of genetic markers for intramuscular fat in the hybrid Wagyu cattle with bulked segregant analysis
title_sort	investigation of genetic markers for intramuscular fat in the hybrid wagyu cattle with bulked segregant analysis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/477bd8600cbb453aae6d9114349a7093
work_keys_str_mv	AT yunzhu investigationofgeneticmarkersforintramuscularfatinthehybridwagyucattlewithbulkedsegregantanalysis AT liyunhan investigationofgeneticmarkersforintramuscularfatinthehybridwagyucattlewithbulkedsegregantanalysis AT pengli investigationofgeneticmarkersforintramuscularfatinthehybridwagyucattlewithbulkedsegregantanalysis AT xiaolongkang investigationofgeneticmarkersforintramuscularfatinthehybridwagyucattlewithbulkedsegregantanalysis AT xingangdan investigationofgeneticmarkersforintramuscularfatinthehybridwagyucattlewithbulkedsegregantanalysis AT yunma investigationofgeneticmarkersforintramuscularfatinthehybridwagyucattlewithbulkedsegregantanalysis AT yuangangshi investigationofgeneticmarkersforintramuscularfatinthehybridwagyucattlewithbulkedsegregantanalysis
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Investigation of genetic markers for intramuscular fat in the hybrid Wagyu cattle with bulked segregant analysis

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