Profiling the Concentration of Reduced and Oxidized Glutathione in Rat Brain Using HPLC/DAD Chromatographic System

This study aimed to develop a HPLC/DAD method in order to determine and quantify the reduced glutathione (GSH) and oxidized glutathione (GSSG) levels in rat brain. Due to the presence of the thiol group (-SH), GSH can interact with the Ellman′s reagent (DTNB), with which it forms a reaction product...

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Autores principales:	George Jîtcă, Erzsébet Fogarasi, Bianca-Eugenia Ősz, Camil Eugen Vari, Ibolya Fülöp, Mircea Dumitru Croitoru, Carmen Maria Rusz, Maria Titica Dogaru
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	oxidative stress glutathione brain liquid chromatography diode array detector Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/477be65d0bc4411aa12b4f3023f0372b
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spelling	oai:doaj.org-article:477be65d0bc4411aa12b4f3023f0372b2021-11-11T18:33:59ZProfiling the Concentration of Reduced and Oxidized Glutathione in Rat Brain Using HPLC/DAD Chromatographic System10.3390/molecules262165901420-3049https://doaj.org/article/477be65d0bc4411aa12b4f3023f0372b2021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6590https://doaj.org/toc/1420-3049This study aimed to develop a HPLC/DAD method in order to determine and quantify the reduced glutathione (GSH) and oxidized glutathione (GSSG) levels in rat brain. Due to the presence of the thiol group (-SH), GSH can interact with the Ellman′s reagent (DTNB), with which it forms a reaction product through which the level of GSH can be quantified, using the DAD detection system. Chromatographic separation was achieved after a derivatization process by using a mobile phase acetonitrile (A) and phosphate buffer (20 mM, pH = 2.5) (B). The compounds of interest were detected at 330 nm using a chromatographic C8 column. The method of determination met the validation criteria, specified by the regulatory bodies. The applicability of the method was demonstrated in a chronic toxicology study of central nervous system (CNS), following different treatment regimens with haloperidol.George JîtcăErzsébet FogarasiBianca-Eugenia ŐszCamil Eugen VariIbolya FülöpMircea Dumitru CroitoruCarmen Maria RuszMaria Titica DogaruMDPI AGarticleoxidative stressglutathionebrainliquid chromatographydiode array detectorOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6590, p 6590 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	oxidative stress glutathione brain liquid chromatography diode array detector Organic chemistry QD241-441
spellingShingle	oxidative stress glutathione brain liquid chromatography diode array detector Organic chemistry QD241-441 George Jîtcă Erzsébet Fogarasi Bianca-Eugenia Ősz Camil Eugen Vari Ibolya Fülöp Mircea Dumitru Croitoru Carmen Maria Rusz Maria Titica Dogaru Profiling the Concentration of Reduced and Oxidized Glutathione in Rat Brain Using HPLC/DAD Chromatographic System
description	This study aimed to develop a HPLC/DAD method in order to determine and quantify the reduced glutathione (GSH) and oxidized glutathione (GSSG) levels in rat brain. Due to the presence of the thiol group (-SH), GSH can interact with the Ellman′s reagent (DTNB), with which it forms a reaction product through which the level of GSH can be quantified, using the DAD detection system. Chromatographic separation was achieved after a derivatization process by using a mobile phase acetonitrile (A) and phosphate buffer (20 mM, pH = 2.5) (B). The compounds of interest were detected at 330 nm using a chromatographic C8 column. The method of determination met the validation criteria, specified by the regulatory bodies. The applicability of the method was demonstrated in a chronic toxicology study of central nervous system (CNS), following different treatment regimens with haloperidol.
format	article
author	George Jîtcă Erzsébet Fogarasi Bianca-Eugenia Ősz Camil Eugen Vari Ibolya Fülöp Mircea Dumitru Croitoru Carmen Maria Rusz Maria Titica Dogaru
author_facet	George Jîtcă Erzsébet Fogarasi Bianca-Eugenia Ősz Camil Eugen Vari Ibolya Fülöp Mircea Dumitru Croitoru Carmen Maria Rusz Maria Titica Dogaru
author_sort	George Jîtcă
title	Profiling the Concentration of Reduced and Oxidized Glutathione in Rat Brain Using HPLC/DAD Chromatographic System
title_short	Profiling the Concentration of Reduced and Oxidized Glutathione in Rat Brain Using HPLC/DAD Chromatographic System
title_full	Profiling the Concentration of Reduced and Oxidized Glutathione in Rat Brain Using HPLC/DAD Chromatographic System
title_fullStr	Profiling the Concentration of Reduced and Oxidized Glutathione in Rat Brain Using HPLC/DAD Chromatographic System
title_full_unstemmed	Profiling the Concentration of Reduced and Oxidized Glutathione in Rat Brain Using HPLC/DAD Chromatographic System
title_sort	profiling the concentration of reduced and oxidized glutathione in rat brain using hplc/dad chromatographic system
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/477be65d0bc4411aa12b4f3023f0372b
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Profiling the Concentration of Reduced and Oxidized Glutathione in Rat Brain Using HPLC/DAD Chromatographic System

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