Full-length transcriptome analysis provides new insights into the early bolting occurrence in medicinal Angelica sinensis

Abstract Angelica sinensis (Oliv.) Diels root part is an integral component of traditional Chinese medicine, widely prescribed to improve blood circulation and blood stasis. However, early bolting of A. sinensis compromises the quality of the roots and hence is a major limitation for yield of medici...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Xue Gao, Fengxia Guo, Yuan Chen, Gang Bai, Yuxiao Liu, Jianqin Jin, Qing Wang
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
Materias:
R
Q
Acceso en línea:https://doaj.org/article/479a4309e0964b62a2b837965db16970
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:479a4309e0964b62a2b837965db16970
record_format dspace
spelling oai:doaj.org-article:479a4309e0964b62a2b837965db169702021-12-02T17:44:55ZFull-length transcriptome analysis provides new insights into the early bolting occurrence in medicinal Angelica sinensis10.1038/s41598-021-92494-42045-2322https://doaj.org/article/479a4309e0964b62a2b837965db169702021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92494-4https://doaj.org/toc/2045-2322Abstract Angelica sinensis (Oliv.) Diels root part is an integral component of traditional Chinese medicine, widely prescribed to improve blood circulation and blood stasis. However, early bolting of A. sinensis compromises the quality of the roots and hence is a major limitation for yield of medicinal materials. To date, little information about the molecular mechanisms underlying bolting is available for this important medicinal plant. To identify genes putatively involved in early bolting, we have conducted the transcriptome analysis of the shoot tips of the early-bolting plants and non-bolting (normal) plants of A. sinensis, respectively, using a combination of third-generation sequencing and next-generation sequencing. A total of 43,438 non-redundant transcripts were collected and 475 unique differentially expressed genes (DEGs) were identified. Gene annotation and functional analyses revealed that DEGs were highly involved in plant hormone signaling and biosynthesis pathways, three main flowering pathways, pollen formation, and very-long-chain fatty acids biosynthesis pathways. The levels of endogenous hormones were also changed significantly in the early bolting stage of A. sinensis. This study provided new insights into the transcriptomic control of early bolting in A. sinensis, which could be further applied to enhance the yield of medicinally important raw materials.Xue GaoFengxia GuoYuan ChenGang BaiYuxiao LiuJianqin JinQing WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Xue Gao
Fengxia Guo
Yuan Chen
Gang Bai
Yuxiao Liu
Jianqin Jin
Qing Wang
Full-length transcriptome analysis provides new insights into the early bolting occurrence in medicinal Angelica sinensis
description Abstract Angelica sinensis (Oliv.) Diels root part is an integral component of traditional Chinese medicine, widely prescribed to improve blood circulation and blood stasis. However, early bolting of A. sinensis compromises the quality of the roots and hence is a major limitation for yield of medicinal materials. To date, little information about the molecular mechanisms underlying bolting is available for this important medicinal plant. To identify genes putatively involved in early bolting, we have conducted the transcriptome analysis of the shoot tips of the early-bolting plants and non-bolting (normal) plants of A. sinensis, respectively, using a combination of third-generation sequencing and next-generation sequencing. A total of 43,438 non-redundant transcripts were collected and 475 unique differentially expressed genes (DEGs) were identified. Gene annotation and functional analyses revealed that DEGs were highly involved in plant hormone signaling and biosynthesis pathways, three main flowering pathways, pollen formation, and very-long-chain fatty acids biosynthesis pathways. The levels of endogenous hormones were also changed significantly in the early bolting stage of A. sinensis. This study provided new insights into the transcriptomic control of early bolting in A. sinensis, which could be further applied to enhance the yield of medicinally important raw materials.
format article
author Xue Gao
Fengxia Guo
Yuan Chen
Gang Bai
Yuxiao Liu
Jianqin Jin
Qing Wang
author_facet Xue Gao
Fengxia Guo
Yuan Chen
Gang Bai
Yuxiao Liu
Jianqin Jin
Qing Wang
author_sort Xue Gao
title Full-length transcriptome analysis provides new insights into the early bolting occurrence in medicinal Angelica sinensis
title_short Full-length transcriptome analysis provides new insights into the early bolting occurrence in medicinal Angelica sinensis
title_full Full-length transcriptome analysis provides new insights into the early bolting occurrence in medicinal Angelica sinensis
title_fullStr Full-length transcriptome analysis provides new insights into the early bolting occurrence in medicinal Angelica sinensis
title_full_unstemmed Full-length transcriptome analysis provides new insights into the early bolting occurrence in medicinal Angelica sinensis
title_sort full-length transcriptome analysis provides new insights into the early bolting occurrence in medicinal angelica sinensis
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/479a4309e0964b62a2b837965db16970
work_keys_str_mv AT xuegao fulllengthtranscriptomeanalysisprovidesnewinsightsintotheearlyboltingoccurrenceinmedicinalangelicasinensis
AT fengxiaguo fulllengthtranscriptomeanalysisprovidesnewinsightsintotheearlyboltingoccurrenceinmedicinalangelicasinensis
AT yuanchen fulllengthtranscriptomeanalysisprovidesnewinsightsintotheearlyboltingoccurrenceinmedicinalangelicasinensis
AT gangbai fulllengthtranscriptomeanalysisprovidesnewinsightsintotheearlyboltingoccurrenceinmedicinalangelicasinensis
AT yuxiaoliu fulllengthtranscriptomeanalysisprovidesnewinsightsintotheearlyboltingoccurrenceinmedicinalangelicasinensis
AT jianqinjin fulllengthtranscriptomeanalysisprovidesnewinsightsintotheearlyboltingoccurrenceinmedicinalangelicasinensis
AT qingwang fulllengthtranscriptomeanalysisprovidesnewinsightsintotheearlyboltingoccurrenceinmedicinalangelicasinensis
_version_ 1718379582957027328