Anti-Cancer Properties of Coix Seed Oil against HT-29 Colon Cells through Regulation of the PI3K/AKT Signaling Pathway

This study aims to observe the effects of coix seed oil (CSO) on HT-29 cells and investigate its possible regulation mechanism of the PI3K/Akt signaling pathway. Fatty acid analysis showed that coix seed oil mainly contains oleic acid (50.54%), linoleic acid (33.76%), palmitic acid (11.74%), and ste...

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Autores principales:	Chunlei Ni, Bailiang Li, Yangyue Ding, Yue Wu, Qiuye Wang, Jiarong Wang, Jianjun Cheng
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	coix seed oil anti-colon cancer cell cycle apoptosis PI3K/AKT Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/479c6ccf16a5458a93a17dfb4cefcbbc
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spelling	oai:doaj.org-article:479c6ccf16a5458a93a17dfb4cefcbbc2021-11-25T17:36:18ZAnti-Cancer Properties of Coix Seed Oil against HT-29 Colon Cells through Regulation of the PI3K/AKT Signaling Pathway10.3390/foods101128332304-8158https://doaj.org/article/479c6ccf16a5458a93a17dfb4cefcbbc2021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2833https://doaj.org/toc/2304-8158This study aims to observe the effects of coix seed oil (CSO) on HT-29 cells and investigate its possible regulation mechanism of the PI3K/Akt signaling pathway. Fatty acid analysis showed that coix seed oil mainly contains oleic acid (50.54%), linoleic acid (33.76%), palmitic acid (11.74%), and stearic acid (2.45%). Fourier transform infrared results found that the fatty acid functional groups present in the oil matched well with the vegetable oil band. The results from CCK-8 assays showed that CSO dose-dependently and time-dependently inhibited the viability of HT-29 cells in vitro. CSO inhibited cell viability, with IC<sub>50</sub> values of 5.30 mg/mL for HT-29 obtained after 24 h treatment. Morphological changes were observed by apoptotic body/cell nucleus DNA (Hoechst 33258) staining using inverted and fluorescence microscopy. Moreover, flow cytometry analysis was used to evaluate the cell cycle and cell apoptosis. It showed that CSO induced cell apoptosis and cycle arrest in the G<sub>2</sub> phase. Quantitative real-time PCR and Western blotting revealed that CSO induced cell apoptosis by downregulating the PI3K/AKT signaling pathway. Additionally, CSO can cause apoptosis in cancer cells by activating caspase-3, up-regulating Bax, and down-regulating Bcl-2. In conclusion, the results revealed that CSO induced G<sub>2</sub> arrest and apoptosis of HT-29 cells by regulating the PI3K/AKT signaling pathway.Chunlei NiBailiang LiYangyue DingYue WuQiuye WangJiarong WangJianjun ChengMDPI AGarticlecoix seed oilanti-colon cancercell cycleapoptosisPI3K/AKTChemical technologyTP1-1185ENFoods, Vol 10, Iss 2833, p 2833 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	coix seed oil anti-colon cancer cell cycle apoptosis PI3K/AKT Chemical technology TP1-1185
spellingShingle	coix seed oil anti-colon cancer cell cycle apoptosis PI3K/AKT Chemical technology TP1-1185 Chunlei Ni Bailiang Li Yangyue Ding Yue Wu Qiuye Wang Jiarong Wang Jianjun Cheng Anti-Cancer Properties of Coix Seed Oil against HT-29 Colon Cells through Regulation of the PI3K/AKT Signaling Pathway
description	This study aims to observe the effects of coix seed oil (CSO) on HT-29 cells and investigate its possible regulation mechanism of the PI3K/Akt signaling pathway. Fatty acid analysis showed that coix seed oil mainly contains oleic acid (50.54%), linoleic acid (33.76%), palmitic acid (11.74%), and stearic acid (2.45%). Fourier transform infrared results found that the fatty acid functional groups present in the oil matched well with the vegetable oil band. The results from CCK-8 assays showed that CSO dose-dependently and time-dependently inhibited the viability of HT-29 cells in vitro. CSO inhibited cell viability, with IC<sub>50</sub> values of 5.30 mg/mL for HT-29 obtained after 24 h treatment. Morphological changes were observed by apoptotic body/cell nucleus DNA (Hoechst 33258) staining using inverted and fluorescence microscopy. Moreover, flow cytometry analysis was used to evaluate the cell cycle and cell apoptosis. It showed that CSO induced cell apoptosis and cycle arrest in the G<sub>2</sub> phase. Quantitative real-time PCR and Western blotting revealed that CSO induced cell apoptosis by downregulating the PI3K/AKT signaling pathway. Additionally, CSO can cause apoptosis in cancer cells by activating caspase-3, up-regulating Bax, and down-regulating Bcl-2. In conclusion, the results revealed that CSO induced G<sub>2</sub> arrest and apoptosis of HT-29 cells by regulating the PI3K/AKT signaling pathway.
format	article
author	Chunlei Ni Bailiang Li Yangyue Ding Yue Wu Qiuye Wang Jiarong Wang Jianjun Cheng
author_facet	Chunlei Ni Bailiang Li Yangyue Ding Yue Wu Qiuye Wang Jiarong Wang Jianjun Cheng
author_sort	Chunlei Ni
title	Anti-Cancer Properties of Coix Seed Oil against HT-29 Colon Cells through Regulation of the PI3K/AKT Signaling Pathway
title_short	Anti-Cancer Properties of Coix Seed Oil against HT-29 Colon Cells through Regulation of the PI3K/AKT Signaling Pathway
title_full	Anti-Cancer Properties of Coix Seed Oil against HT-29 Colon Cells through Regulation of the PI3K/AKT Signaling Pathway
title_fullStr	Anti-Cancer Properties of Coix Seed Oil against HT-29 Colon Cells through Regulation of the PI3K/AKT Signaling Pathway
title_full_unstemmed	Anti-Cancer Properties of Coix Seed Oil against HT-29 Colon Cells through Regulation of the PI3K/AKT Signaling Pathway
title_sort	anti-cancer properties of coix seed oil against ht-29 colon cells through regulation of the pi3k/akt signaling pathway
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/479c6ccf16a5458a93a17dfb4cefcbbc
work_keys_str_mv	AT chunleini anticancerpropertiesofcoixseedoilagainstht29coloncellsthroughregulationofthepi3kaktsignalingpathway AT bailiangli anticancerpropertiesofcoixseedoilagainstht29coloncellsthroughregulationofthepi3kaktsignalingpathway AT yangyueding anticancerpropertiesofcoixseedoilagainstht29coloncellsthroughregulationofthepi3kaktsignalingpathway AT yuewu anticancerpropertiesofcoixseedoilagainstht29coloncellsthroughregulationofthepi3kaktsignalingpathway AT qiuyewang anticancerpropertiesofcoixseedoilagainstht29coloncellsthroughregulationofthepi3kaktsignalingpathway AT jiarongwang anticancerpropertiesofcoixseedoilagainstht29coloncellsthroughregulationofthepi3kaktsignalingpathway AT jianjuncheng anticancerpropertiesofcoixseedoilagainstht29coloncellsthroughregulationofthepi3kaktsignalingpathway
_version_	1718412161632436224

Anti-Cancer Properties of Coix Seed Oil against HT-29 Colon Cells through Regulation of the PI3K/AKT Signaling Pathway

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