<named-content content-type="genus-species">Escherichia coli</named-content> Has a Unique Transcriptional Program in Long-Term Stationary Phase Allowing Identification of Genes Important for Survival

ABSTRACT Microbes live in complex and constantly changing environments, but it is difficult to replicate this in the laboratory. Escherichia coli has been used as a model organism in experimental evolution studies for years; specifically, we and others have used it to study evolution in complex envi...

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Autores principales:	Karin E. Kram, Autumn L. Henderson, Steven E. Finkel
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	cold shock experimental evolution genetics long-term stationary phase transcriptomics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/479d961923b64cc2a82b2eb6d0e21d56
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spelling	oai:doaj.org-article:479d961923b64cc2a82b2eb6d0e21d562021-12-02T18:15:46Z<named-content content-type="genus-species">Escherichia coli</named-content> Has a Unique Transcriptional Program in Long-Term Stationary Phase Allowing Identification of Genes Important for Survival10.1128/mSystems.00364-202379-5077https://doaj.org/article/479d961923b64cc2a82b2eb6d0e21d562020-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00364-20https://doaj.org/toc/2379-5077ABSTRACT Microbes live in complex and constantly changing environments, but it is difficult to replicate this in the laboratory. Escherichia coli has been used as a model organism in experimental evolution studies for years; specifically, we and others have used it to study evolution in complex environments by incubating the cells into long-term stationary phase (LTSP) in rich media. In LTSP, cells experience a variety of stresses and changing conditions. While we have hypothesized that this experimental system is more similar to natural environments than some other lab conditions, we do not yet know how cells respond to this environment biochemically or physiologically. In this study, we began to unravel the cells’ responses to this environment by characterizing the transcriptome of cells during LTSP. We found that cells in LTSP have a unique transcriptional program and that several genes are uniquely upregulated or downregulated in this phase. Further, we identified two genes, cspB and cspI, which are most highly expressed in LTSP, even though these genes are primarily known to respond to cold shock. By competing cells lacking these genes with wild-type cells, we show that these genes are also important for survival during LTSP. These data can help identify gene products that may play a role in survival in this complex environment and lead to identification of novel functions of proteins. IMPORTANCE Experimental evolution studies have elucidated evolutionary processes, but usually in chemically well-defined and/or constant environments. Using complex environments is important to begin to understand how evolution may occur in natural environments, such as soils or within a host. However, characterizing the stresses that cells experience in these complex environments can be challenging. One way to approach this is by determining how cells biochemically acclimate to heterogenous environments. In this study, we began to characterize physiological changes by analyzing the transcriptome of cells in a dynamic complex environment. By characterizing the transcriptional profile of cells in long-term stationary phase, a heterogenous and stressful environment, we can begin to understand how cells physiologically and biochemically react to the laboratory environment, and how this compares to more-natural conditions.Karin E. KramAutumn L. HendersonSteven E. FinkelAmerican Society for Microbiologyarticlecold shockexperimental evolutiongeneticslong-term stationary phasetranscriptomicsMicrobiologyQR1-502ENmSystems, Vol 5, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	cold shock experimental evolution genetics long-term stationary phase transcriptomics Microbiology QR1-502
spellingShingle	cold shock experimental evolution genetics long-term stationary phase transcriptomics Microbiology QR1-502 Karin E. Kram Autumn L. Henderson Steven E. Finkel <named-content content-type="genus-species">Escherichia coli</named-content> Has a Unique Transcriptional Program in Long-Term Stationary Phase Allowing Identification of Genes Important for Survival
description	ABSTRACT Microbes live in complex and constantly changing environments, but it is difficult to replicate this in the laboratory. Escherichia coli has been used as a model organism in experimental evolution studies for years; specifically, we and others have used it to study evolution in complex environments by incubating the cells into long-term stationary phase (LTSP) in rich media. In LTSP, cells experience a variety of stresses and changing conditions. While we have hypothesized that this experimental system is more similar to natural environments than some other lab conditions, we do not yet know how cells respond to this environment biochemically or physiologically. In this study, we began to unravel the cells’ responses to this environment by characterizing the transcriptome of cells during LTSP. We found that cells in LTSP have a unique transcriptional program and that several genes are uniquely upregulated or downregulated in this phase. Further, we identified two genes, cspB and cspI, which are most highly expressed in LTSP, even though these genes are primarily known to respond to cold shock. By competing cells lacking these genes with wild-type cells, we show that these genes are also important for survival during LTSP. These data can help identify gene products that may play a role in survival in this complex environment and lead to identification of novel functions of proteins. IMPORTANCE Experimental evolution studies have elucidated evolutionary processes, but usually in chemically well-defined and/or constant environments. Using complex environments is important to begin to understand how evolution may occur in natural environments, such as soils or within a host. However, characterizing the stresses that cells experience in these complex environments can be challenging. One way to approach this is by determining how cells biochemically acclimate to heterogenous environments. In this study, we began to characterize physiological changes by analyzing the transcriptome of cells in a dynamic complex environment. By characterizing the transcriptional profile of cells in long-term stationary phase, a heterogenous and stressful environment, we can begin to understand how cells physiologically and biochemically react to the laboratory environment, and how this compares to more-natural conditions.
format	article
author	Karin E. Kram Autumn L. Henderson Steven E. Finkel
author_facet	Karin E. Kram Autumn L. Henderson Steven E. Finkel
author_sort	Karin E. Kram
title	<named-content content-type="genus-species">Escherichia coli</named-content> Has a Unique Transcriptional Program in Long-Term Stationary Phase Allowing Identification of Genes Important for Survival
title_short	<named-content content-type="genus-species">Escherichia coli</named-content> Has a Unique Transcriptional Program in Long-Term Stationary Phase Allowing Identification of Genes Important for Survival
title_full	<named-content content-type="genus-species">Escherichia coli</named-content> Has a Unique Transcriptional Program in Long-Term Stationary Phase Allowing Identification of Genes Important for Survival
title_fullStr	<named-content content-type="genus-species">Escherichia coli</named-content> Has a Unique Transcriptional Program in Long-Term Stationary Phase Allowing Identification of Genes Important for Survival
title_full_unstemmed	<named-content content-type="genus-species">Escherichia coli</named-content> Has a Unique Transcriptional Program in Long-Term Stationary Phase Allowing Identification of Genes Important for Survival
title_sort	<named-content content-type="genus-species">escherichia coli</named-content> has a unique transcriptional program in long-term stationary phase allowing identification of genes important for survival
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/479d961923b64cc2a82b2eb6d0e21d56
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<named-content content-type="genus-species">Escherichia coli</named-content> Has a Unique Transcriptional Program in Long-Term Stationary Phase Allowing Identification of Genes Important for Survival

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