Cytokine induction of sol–gel-derived TiO2 and SiO2 coatings on metallic substrates after implantation to rat femur
Wiktor Urbanski,1 Krzysztof Marycz,2 Justyna Krzak,3 Celina Pezowicz,4 Szymon Feliks Dragan1 1Department of Orthopaedic Surgery and Traumatology, Wroclaw University Hospital, 2Electron Microscope Laboratory, Wroclaw University of Environmental and Life Sciences, 3Institute of Materials Science and...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2017
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/47a10589c768480ebc9f2b48891229b2 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:47a10589c768480ebc9f2b48891229b2 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:47a10589c768480ebc9f2b48891229b22021-12-02T05:02:52ZCytokine induction of sol–gel-derived TiO2 and SiO2 coatings on metallic substrates after implantation to rat femur1178-2013https://doaj.org/article/47a10589c768480ebc9f2b48891229b22017-02-01T00:00:00Zhttps://www.dovepress.com/cytokine-induction-of-solndashgel-derived-tio2-and-sio2-coatings-on-me-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Wiktor Urbanski,1 Krzysztof Marycz,2 Justyna Krzak,3 Celina Pezowicz,4 Szymon Feliks Dragan1 1Department of Orthopaedic Surgery and Traumatology, Wroclaw University Hospital, 2Electron Microscope Laboratory, Wroclaw University of Environmental and Life Sciences, 3Institute of Materials Science and Applied Mechanics, 4Division of Biomedical Engineering and Experimental Mechanics, Wroclaw University of Technology, Wroclaw, Poland Abstract: Material surface is a key determinant of host response on implanted biomaterial. Therefore, modification of the implant surface may optimize implant–tissue reactions. Inflammatory reaction is inevitable after biomaterial implantation, but prolonged inflammation may lead to adverse reactions and subsequent implant failure. Proinflammatory activities of cytokines like interleukin (IL)-1, IL-6, and tumor necrosis factor-alpha (TNF-α) are attractive indicators of these processes and ultimately characterize biocompatibility. The objective of the study was to evaluate local cytokine production after implantation of stainless steel 316L (SS) and titanium alloy (Ti6Al4V) biomaterials coated with titanium dioxide (TiO2) and silica (SiO2) coatings prepared by sol–gel method. Biomaterials were implanted into rat femur and after 12 weeks, bones were harvested. Bone–implant tissue interface was evaluated; immunohistochemical staining was performed to identify IL-6, TNF-α, and Caspase-1. Histomorphometry (AxioVision Rel. 4.6.3 software) of tissue samples was performed in order to quantify the cytokine levels. Both the oxide coatings on SS and Ti6Al4V significantly reduced cytokine production. However, the lowest cytokine levels were observed in TiO2 groups. Cytokine content in uncoated groups was lower in Ti6Al4V than in SS, although coating of either metal reduced cytokine production to similar levels. Sol–gel TiO2 or SiO2 coatings reduced significantly the production of proinflammatory cytokines by local tissues, irrespective of the material used as a substrate, that is, either Ti6Al4V or SS. This suggests lower inflammatory response, which directly points out improvement of materials’ biocompatibility. Keywords: bone implant, surface modification, sol–gel coatings, inflammation, biomaterialUrbanski WMarycz KKrzak JPezowicz CDragan SFDove Medical Pressarticlesol-gel coatingsbone implantcytokineinflammationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 1639-1645 (2017) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
sol-gel coatings bone implant cytokine inflammation Medicine (General) R5-920 |
| spellingShingle |
sol-gel coatings bone implant cytokine inflammation Medicine (General) R5-920 Urbanski W Marycz K Krzak J Pezowicz C Dragan SF Cytokine induction of sol–gel-derived TiO2 and SiO2 coatings on metallic substrates after implantation to rat femur |
| description |
Wiktor Urbanski,1 Krzysztof Marycz,2 Justyna Krzak,3 Celina Pezowicz,4 Szymon Feliks Dragan1 1Department of Orthopaedic Surgery and Traumatology, Wroclaw University Hospital, 2Electron Microscope Laboratory, Wroclaw University of Environmental and Life Sciences, 3Institute of Materials Science and Applied Mechanics, 4Division of Biomedical Engineering and Experimental Mechanics, Wroclaw University of Technology, Wroclaw, Poland Abstract: Material surface is a key determinant of host response on implanted biomaterial. Therefore, modification of the implant surface may optimize implant–tissue reactions. Inflammatory reaction is inevitable after biomaterial implantation, but prolonged inflammation may lead to adverse reactions and subsequent implant failure. Proinflammatory activities of cytokines like interleukin (IL)-1, IL-6, and tumor necrosis factor-alpha (TNF-α) are attractive indicators of these processes and ultimately characterize biocompatibility. The objective of the study was to evaluate local cytokine production after implantation of stainless steel 316L (SS) and titanium alloy (Ti6Al4V) biomaterials coated with titanium dioxide (TiO2) and silica (SiO2) coatings prepared by sol–gel method. Biomaterials were implanted into rat femur and after 12 weeks, bones were harvested. Bone–implant tissue interface was evaluated; immunohistochemical staining was performed to identify IL-6, TNF-α, and Caspase-1. Histomorphometry (AxioVision Rel. 4.6.3 software) of tissue samples was performed in order to quantify the cytokine levels. Both the oxide coatings on SS and Ti6Al4V significantly reduced cytokine production. However, the lowest cytokine levels were observed in TiO2 groups. Cytokine content in uncoated groups was lower in Ti6Al4V than in SS, although coating of either metal reduced cytokine production to similar levels. Sol–gel TiO2 or SiO2 coatings reduced significantly the production of proinflammatory cytokines by local tissues, irrespective of the material used as a substrate, that is, either Ti6Al4V or SS. This suggests lower inflammatory response, which directly points out improvement of materials’ biocompatibility. Keywords: bone implant, surface modification, sol–gel coatings, inflammation, biomaterial |
| format |
article |
| author |
Urbanski W Marycz K Krzak J Pezowicz C Dragan SF |
| author_facet |
Urbanski W Marycz K Krzak J Pezowicz C Dragan SF |
| author_sort |
Urbanski W |
| title |
Cytokine induction of sol–gel-derived TiO2 and SiO2 coatings on metallic substrates after implantation to rat femur |
| title_short |
Cytokine induction of sol–gel-derived TiO2 and SiO2 coatings on metallic substrates after implantation to rat femur |
| title_full |
Cytokine induction of sol–gel-derived TiO2 and SiO2 coatings on metallic substrates after implantation to rat femur |
| title_fullStr |
Cytokine induction of sol–gel-derived TiO2 and SiO2 coatings on metallic substrates after implantation to rat femur |
| title_full_unstemmed |
Cytokine induction of sol–gel-derived TiO2 and SiO2 coatings on metallic substrates after implantation to rat femur |
| title_sort |
cytokine induction of sol–gel-derived tio2 and sio2 coatings on metallic substrates after implantation to rat femur |
| publisher |
Dove Medical Press |
| publishDate |
2017 |
| url |
https://doaj.org/article/47a10589c768480ebc9f2b48891229b2 |
| work_keys_str_mv |
AT urbanskiw cytokineinductionofsolndashgelderivedtio2andsio2coatingsonmetallicsubstratesafterimplantationtoratfemur AT maryczk cytokineinductionofsolndashgelderivedtio2andsio2coatingsonmetallicsubstratesafterimplantationtoratfemur AT krzakj cytokineinductionofsolndashgelderivedtio2andsio2coatingsonmetallicsubstratesafterimplantationtoratfemur AT pezowiczc cytokineinductionofsolndashgelderivedtio2andsio2coatingsonmetallicsubstratesafterimplantationtoratfemur AT dragansf cytokineinductionofsolndashgelderivedtio2andsio2coatingsonmetallicsubstratesafterimplantationtoratfemur |
| _version_ |
1718400705903984640 |