Highly efficient human serum filtration with water-soluble nanoporous nanoparticles

Antonella Pujia1, Francesco De Angelis1,2, Domenica Scumaci3, Marco Gaspari3, Carlo Liberale1,2, Patrizio Candeloro1, Giovanni Cuda3, Enzo Di Fabrizio1,21BIONEM Laboratory, Department of Experimental and Clinical Medicine, University of Catanzaro &ldquo;Magna Graecia&rdquo;, Germanet...

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Autores principales:	Antonella Pujia, Francesco De Angelis, Domenica Scumaci, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/47adee606646417ca42908d12c64758d
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spelling	oai:doaj.org-article:47adee606646417ca42908d12c64758d2021-12-02T01:32:58ZHighly efficient human serum filtration with water-soluble nanoporous nanoparticles1176-91141178-2013https://doaj.org/article/47adee606646417ca42908d12c64758d2010-11-01T00:00:00Zhttp://www.dovepress.com/highly-efficient-human-serum-filtration-with-water-soluble-nanoporous--a5690https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Antonella Pujia1, Francesco De Angelis1,2, Domenica Scumaci3, Marco Gaspari3, Carlo Liberale1,2, Patrizio Candeloro1, Giovanni Cuda3, Enzo Di Fabrizio1,21BIONEM Laboratory, Department of Experimental and Clinical Medicine, University of Catanzaro &ldquo;Magna Graecia&rdquo;, Germaneto (CZ), Italy; 2IIT, Italian Institute of Technology, Genova, Italy; 3Proteomics and Mass Spectrometry Laboratory, Department of Experimental and Clinical Medicine, University of Catanzaro &ldquo;Magna Graecia&rdquo;, Germaneto (CZ), ItalyBackground: Human serum has the potential to become the most informative source of novel biomarkers, but its study is very difficult due to the incredible complexity of its molecular composition. We describe a novel tool based on biodegradable nanoporous nanoparticles (NPNPs) that allows the harvesting of low-molecular-weight fractions of crude human serum or other biofluids. NPNPs with a diameter of 200 nm and pore size of a few nm were obtained by ultrasonication of nanoporous silicon. When incubated with a solution, the NPNPs harvest only the molecules small enough to be absorbed into the nanopores. Then they can be recovered by centrifugation and dissolved in water, making the harvested molecules available for further analyses.Results: Fluorescence microscopy, gel electrophoresis, and mass spectrometry were used to show the enrichment of low-molecular-weight fraction of serum under physiological conditions, with a cut-off of 13 kDa and an enrichment factor &gt;50.Conclusion: From these findings, we conclude that ability to tune pore size, combined with the availability of hundreds of biomolecule cross-linkers, opens up new perspectives on complex biofluid analysis, discovery of biomarkers, and in situ drug delivery.Keywords: nanoporous silicon, nanoparticle, biomarker discovery, human serum proteomics, harvesting Antonella PujiaFrancesco De AngelisDomenica Scumaciet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 1005-1015 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Antonella Pujia Francesco De Angelis Domenica Scumaci et al Highly efficient human serum filtration with water-soluble nanoporous nanoparticles
description	Antonella Pujia1, Francesco De Angelis1,2, Domenica Scumaci3, Marco Gaspari3, Carlo Liberale1,2, Patrizio Candeloro1, Giovanni Cuda3, Enzo Di Fabrizio1,21BIONEM Laboratory, Department of Experimental and Clinical Medicine, University of Catanzaro &ldquo;Magna Graecia&rdquo;, Germaneto (CZ), Italy; 2IIT, Italian Institute of Technology, Genova, Italy; 3Proteomics and Mass Spectrometry Laboratory, Department of Experimental and Clinical Medicine, University of Catanzaro &ldquo;Magna Graecia&rdquo;, Germaneto (CZ), ItalyBackground: Human serum has the potential to become the most informative source of novel biomarkers, but its study is very difficult due to the incredible complexity of its molecular composition. We describe a novel tool based on biodegradable nanoporous nanoparticles (NPNPs) that allows the harvesting of low-molecular-weight fractions of crude human serum or other biofluids. NPNPs with a diameter of 200 nm and pore size of a few nm were obtained by ultrasonication of nanoporous silicon. When incubated with a solution, the NPNPs harvest only the molecules small enough to be absorbed into the nanopores. Then they can be recovered by centrifugation and dissolved in water, making the harvested molecules available for further analyses.Results: Fluorescence microscopy, gel electrophoresis, and mass spectrometry were used to show the enrichment of low-molecular-weight fraction of serum under physiological conditions, with a cut-off of 13 kDa and an enrichment factor &gt;50.Conclusion: From these findings, we conclude that ability to tune pore size, combined with the availability of hundreds of biomolecule cross-linkers, opens up new perspectives on complex biofluid analysis, discovery of biomarkers, and in situ drug delivery.Keywords: nanoporous silicon, nanoparticle, biomarker discovery, human serum proteomics, harvesting
format	article
author	Antonella Pujia Francesco De Angelis Domenica Scumaci et al
author_facet	Antonella Pujia Francesco De Angelis Domenica Scumaci et al
author_sort	Antonella Pujia
title	Highly efficient human serum filtration with water-soluble nanoporous nanoparticles
title_short	Highly efficient human serum filtration with water-soluble nanoporous nanoparticles
title_full	Highly efficient human serum filtration with water-soluble nanoporous nanoparticles
title_fullStr	Highly efficient human serum filtration with water-soluble nanoporous nanoparticles
title_full_unstemmed	Highly efficient human serum filtration with water-soluble nanoporous nanoparticles
title_sort	highly efficient human serum filtration with water-soluble nanoporous nanoparticles
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/47adee606646417ca42908d12c64758d
work_keys_str_mv	AT antonellapujia highlyefficienthumanserumfiltrationwithwatersolublenanoporousnanoparticles AT francescodeangelis highlyefficienthumanserumfiltrationwithwatersolublenanoporousnanoparticles AT domenicascumaci highlyefficienthumanserumfiltrationwithwatersolublenanoporousnanoparticles AT etal highlyefficienthumanserumfiltrationwithwatersolublenanoporousnanoparticles
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Highly efficient human serum filtration with water-soluble nanoporous nanoparticles

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