Liver transcriptome changes in zebrafish during acclimation to transport-associated stress.

Liver plays a key role during the stress acclimation, and liver transcriptome analysis of shipped zebrafish could reveal the molecular adjustments that occur in the organ. Transcriptional changes in liver were analyzed with a 44 K oligo array using total RNA from fish prior to transport and during a...

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Autores principales:	Anusha K S Dhanasiri, Jorge M O Fernandes, Viswanath Kiron
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/47af3befa65545558db839138b542aba
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spelling	oai:doaj.org-article:47af3befa65545558db839138b542aba2021-11-18T07:42:30ZLiver transcriptome changes in zebrafish during acclimation to transport-associated stress.1932-620310.1371/journal.pone.0065028https://doaj.org/article/47af3befa65545558db839138b542aba2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23762281/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Liver plays a key role during the stress acclimation, and liver transcriptome analysis of shipped zebrafish could reveal the molecular adjustments that occur in the organ. Transcriptional changes in liver were analyzed with a 44 K oligo array using total RNA from fish prior to transport and during a mock transport process--immediately after packing (0 h), at 48 and 72 h. Large numbers of genes related to a variety of biological processes and pathways were regulated, mainly during transport (at 48/72 h). Immediately after packing, transcripts of genes related to both gluconeogenesis and glycolysis were induced. During transport, induction of gluconeogenesis-linked genes and reduction of glycolysis-related genes may be supporting the increase in blood glucose levels. Inhibition of genes involved in fatty acid beta-oxidation may be pointing to the poor ability of fish to utilize energy from fatty acids, under transport conditions. Genes involved in some of the mechanisms that regulate body ammonia were also affected. Even though genes associated with certain transaminases were inhibited in liver, sustained glutamate deamination may have led to high ammonia accumulation in liver/body. Enhanced levels of gene transcripts in ubiquitination and MAPK signalling cascade and reduced levels of gene transcripts related to ROS generation via peroxisomal enzymes as well as xenobiotic metabolism may be signifying the importance of such cellular and tissue responses to maintain homeostasis. Furthermore, transcripts connected with stress and thyroid hormones were also regulated. Moreover, suppression of genes related to specific immune components may be denoting the deleterious impact of transport on fish health. Thus, this study has revealed the complex molecular adjustments that occur in zebrafish when they are transported.Anusha K S DhanasiriJorge M O FernandesViswanath KironPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e65028 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anusha K S Dhanasiri Jorge M O Fernandes Viswanath Kiron Liver transcriptome changes in zebrafish during acclimation to transport-associated stress.
description	Liver plays a key role during the stress acclimation, and liver transcriptome analysis of shipped zebrafish could reveal the molecular adjustments that occur in the organ. Transcriptional changes in liver were analyzed with a 44 K oligo array using total RNA from fish prior to transport and during a mock transport process--immediately after packing (0 h), at 48 and 72 h. Large numbers of genes related to a variety of biological processes and pathways were regulated, mainly during transport (at 48/72 h). Immediately after packing, transcripts of genes related to both gluconeogenesis and glycolysis were induced. During transport, induction of gluconeogenesis-linked genes and reduction of glycolysis-related genes may be supporting the increase in blood glucose levels. Inhibition of genes involved in fatty acid beta-oxidation may be pointing to the poor ability of fish to utilize energy from fatty acids, under transport conditions. Genes involved in some of the mechanisms that regulate body ammonia were also affected. Even though genes associated with certain transaminases were inhibited in liver, sustained glutamate deamination may have led to high ammonia accumulation in liver/body. Enhanced levels of gene transcripts in ubiquitination and MAPK signalling cascade and reduced levels of gene transcripts related to ROS generation via peroxisomal enzymes as well as xenobiotic metabolism may be signifying the importance of such cellular and tissue responses to maintain homeostasis. Furthermore, transcripts connected with stress and thyroid hormones were also regulated. Moreover, suppression of genes related to specific immune components may be denoting the deleterious impact of transport on fish health. Thus, this study has revealed the complex molecular adjustments that occur in zebrafish when they are transported.
format	article
author	Anusha K S Dhanasiri Jorge M O Fernandes Viswanath Kiron
author_facet	Anusha K S Dhanasiri Jorge M O Fernandes Viswanath Kiron
author_sort	Anusha K S Dhanasiri
title	Liver transcriptome changes in zebrafish during acclimation to transport-associated stress.
title_short	Liver transcriptome changes in zebrafish during acclimation to transport-associated stress.
title_full	Liver transcriptome changes in zebrafish during acclimation to transport-associated stress.
title_fullStr	Liver transcriptome changes in zebrafish during acclimation to transport-associated stress.
title_full_unstemmed	Liver transcriptome changes in zebrafish during acclimation to transport-associated stress.
title_sort	liver transcriptome changes in zebrafish during acclimation to transport-associated stress.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/47af3befa65545558db839138b542aba
work_keys_str_mv	AT anushaksdhanasiri livertranscriptomechangesinzebrafishduringacclimationtotransportassociatedstress AT jorgemofernandes livertranscriptomechangesinzebrafishduringacclimationtotransportassociatedstress AT viswanathkiron livertranscriptomechangesinzebrafishduringacclimationtotransportassociatedstress
_version_	1718423094292381696

Liver transcriptome changes in zebrafish during acclimation to transport-associated stress.

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