Population authentication of the traditional medicinal plant Cassia tora L. based on ISSR markers and FTIR analysis

Abstract Cassia tora is a plant of medicinal importance. Medicinal plants from different localities are believed to differ in their therapeutic potency. In this study, six populations of C. tora with different eco-geographical origins were investigated genotypically (ISSR) and phytochemically (FTIR)...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Vikas Kumar, Bijoy Krishna Roy
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/47b36548f2044ef19dbb537fd6dd88fb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:47b36548f2044ef19dbb537fd6dd88fb
record_format	dspace
spelling	oai:doaj.org-article:47b36548f2044ef19dbb537fd6dd88fb2021-12-02T15:07:47ZPopulation authentication of the traditional medicinal plant Cassia tora L. based on ISSR markers and FTIR analysis10.1038/s41598-018-29114-12045-2322https://doaj.org/article/47b36548f2044ef19dbb537fd6dd88fb2018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-29114-1https://doaj.org/toc/2045-2322Abstract Cassia tora is a plant of medicinal importance. Medicinal plants from different localities are believed to differ in their therapeutic potency. In this study, six populations of C. tora with different eco-geographical origins were investigated genotypically (ISSR) and phytochemically (FTIR) to establish an integrated approach for population discrimination and authentication of the origin of this medicinal herb. CHS gene expression analysis and determination of flavonoid content were carried out to substantiate the study. A total of 19 population-specific authentication bands were observed in 11 ISSR fingerprints. Authentication codes were generated using six highly polymorphic bands, including three authentication bands. FTIR spectra revealed that the peaks at wavenumber 1623 cm−1 (carbonyl group) and 1034 cm−1 (>CO- group) were powerful in separating the populations. These peaks are assigned to flavonoids and carbohydrates, respectively, were more intense for Ranchi (highland) population. Variation in the transcript level of CHS gene was observed. The findings of FTIR and RT-PCR analyses were in agreement with the TFC analysis, where, the lowest amount of flavonoids observed for Lucknow (lowland) population. All the populations of C. tora have been authenticated accurately by ISSR analyses and FTIR fingerprinting, and the Ranchi site was observed to be more suitable for the potential harvesting of therapeutic bioactive compounds.Vikas KumarBijoy Krishna RoyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vikas Kumar Bijoy Krishna Roy Population authentication of the traditional medicinal plant Cassia tora L. based on ISSR markers and FTIR analysis
description	Abstract Cassia tora is a plant of medicinal importance. Medicinal plants from different localities are believed to differ in their therapeutic potency. In this study, six populations of C. tora with different eco-geographical origins were investigated genotypically (ISSR) and phytochemically (FTIR) to establish an integrated approach for population discrimination and authentication of the origin of this medicinal herb. CHS gene expression analysis and determination of flavonoid content were carried out to substantiate the study. A total of 19 population-specific authentication bands were observed in 11 ISSR fingerprints. Authentication codes were generated using six highly polymorphic bands, including three authentication bands. FTIR spectra revealed that the peaks at wavenumber 1623 cm−1 (carbonyl group) and 1034 cm−1 (>CO- group) were powerful in separating the populations. These peaks are assigned to flavonoids and carbohydrates, respectively, were more intense for Ranchi (highland) population. Variation in the transcript level of CHS gene was observed. The findings of FTIR and RT-PCR analyses were in agreement with the TFC analysis, where, the lowest amount of flavonoids observed for Lucknow (lowland) population. All the populations of C. tora have been authenticated accurately by ISSR analyses and FTIR fingerprinting, and the Ranchi site was observed to be more suitable for the potential harvesting of therapeutic bioactive compounds.
format	article
author	Vikas Kumar Bijoy Krishna Roy
author_facet	Vikas Kumar Bijoy Krishna Roy
author_sort	Vikas Kumar
title	Population authentication of the traditional medicinal plant Cassia tora L. based on ISSR markers and FTIR analysis
title_short	Population authentication of the traditional medicinal plant Cassia tora L. based on ISSR markers and FTIR analysis
title_full	Population authentication of the traditional medicinal plant Cassia tora L. based on ISSR markers and FTIR analysis
title_fullStr	Population authentication of the traditional medicinal plant Cassia tora L. based on ISSR markers and FTIR analysis
title_full_unstemmed	Population authentication of the traditional medicinal plant Cassia tora L. based on ISSR markers and FTIR analysis
title_sort	population authentication of the traditional medicinal plant cassia tora l. based on issr markers and ftir analysis
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/47b36548f2044ef19dbb537fd6dd88fb
work_keys_str_mv	AT vikaskumar populationauthenticationofthetraditionalmedicinalplantcassiatoralbasedonissrmarkersandftiranalysis AT bijoykrishnaroy populationauthenticationofthetraditionalmedicinalplantcassiatoralbasedonissrmarkersandftiranalysis
_version_	1718388381183901696

Population authentication of the traditional medicinal plant Cassia tora L. based on ISSR markers and FTIR analysis

Ejemplares similares