16S rRNA gene-based microbiome analysis identifies candidate bacterial strains that increase the storage time of potato tubers

Abstract In the past, the potato plant microbiota and rhizosphere have been studied in detail to improve plant growth and fitness. However, less is known about the postharvest potato tuber microbiome and its role in storage stability. The storage stability of potatoes depends on genotype and storage...

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Autores principales:	Franziska Buchholz, Robert Junker, Abdul Samad, Livio Antonielli, Nataša Sarić, Tanja Kostić, Angela Sessitsch, Birgit Mitter
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/47b49062be6d455ba2aee92be0505855
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spelling	oai:doaj.org-article:47b49062be6d455ba2aee92be05058552021-12-02T14:06:11Z16S rRNA gene-based microbiome analysis identifies candidate bacterial strains that increase the storage time of potato tubers10.1038/s41598-021-82181-92045-2322https://doaj.org/article/47b49062be6d455ba2aee92be05058552021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82181-9https://doaj.org/toc/2045-2322Abstract In the past, the potato plant microbiota and rhizosphere have been studied in detail to improve plant growth and fitness. However, less is known about the postharvest potato tuber microbiome and its role in storage stability. The storage stability of potatoes depends on genotype and storage conditions, but the soil in which tubers were grown could also play a role. To understand the ecology and functional role of the postharvest potato microbiota, we planted four potato varieties in five soil types and monitored them until the tubers started sprouting. During storage, the bacterial community of tubers was analysed by next-generation sequencing of the 16S rRNA gene amplicons. The potato tubers exhibited soil-dependent differences in sprouting behaviour. The statistical analysis revealed a strong shift of the tuber-associated bacterial community from harvest to dormancy break. By combining indicator species analysis and a correlation matrix, we predicted associations between members of the bacterial community and tuber sprouting behaviour. Based on this, we identified Flavobacterium sp. isolates, which were able to influence sprouting behaviour by inhibiting potato bud outgrowth.Franziska BuchholzRobert JunkerAbdul SamadLivio AntonielliNataša SarićTanja KostićAngela SessitschBirgit MitterNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Franziska Buchholz Robert Junker Abdul Samad Livio Antonielli Nataša Sarić Tanja Kostić Angela Sessitsch Birgit Mitter 16S rRNA gene-based microbiome analysis identifies candidate bacterial strains that increase the storage time of potato tubers
description	Abstract In the past, the potato plant microbiota and rhizosphere have been studied in detail to improve plant growth and fitness. However, less is known about the postharvest potato tuber microbiome and its role in storage stability. The storage stability of potatoes depends on genotype and storage conditions, but the soil in which tubers were grown could also play a role. To understand the ecology and functional role of the postharvest potato microbiota, we planted four potato varieties in five soil types and monitored them until the tubers started sprouting. During storage, the bacterial community of tubers was analysed by next-generation sequencing of the 16S rRNA gene amplicons. The potato tubers exhibited soil-dependent differences in sprouting behaviour. The statistical analysis revealed a strong shift of the tuber-associated bacterial community from harvest to dormancy break. By combining indicator species analysis and a correlation matrix, we predicted associations between members of the bacterial community and tuber sprouting behaviour. Based on this, we identified Flavobacterium sp. isolates, which were able to influence sprouting behaviour by inhibiting potato bud outgrowth.
format	article
author	Franziska Buchholz Robert Junker Abdul Samad Livio Antonielli Nataša Sarić Tanja Kostić Angela Sessitsch Birgit Mitter
author_facet	Franziska Buchholz Robert Junker Abdul Samad Livio Antonielli Nataša Sarić Tanja Kostić Angela Sessitsch Birgit Mitter
author_sort	Franziska Buchholz
title	16S rRNA gene-based microbiome analysis identifies candidate bacterial strains that increase the storage time of potato tubers
title_short	16S rRNA gene-based microbiome analysis identifies candidate bacterial strains that increase the storage time of potato tubers
title_full	16S rRNA gene-based microbiome analysis identifies candidate bacterial strains that increase the storage time of potato tubers
title_fullStr	16S rRNA gene-based microbiome analysis identifies candidate bacterial strains that increase the storage time of potato tubers
title_full_unstemmed	16S rRNA gene-based microbiome analysis identifies candidate bacterial strains that increase the storage time of potato tubers
title_sort	16s rrna gene-based microbiome analysis identifies candidate bacterial strains that increase the storage time of potato tubers
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/47b49062be6d455ba2aee92be0505855
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16S rRNA gene-based microbiome analysis identifies candidate bacterial strains that increase the storage time of potato tubers

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