NEW WAY OF ISOLATION OF ENZYME COMPLEX OF CHOLERA VIBRIO - PROTEOVIBRIN BY MEANS OF ULTRAFILTRATION

The technology of isolation of the cholera vibrio complex of low-molecular enzymes related to pathogenicity factors, from cholera vaccine production wastes by means of concentrating ultrafiltration was developed. This technology allows to obtain standard series of the preparation termed proteovibrin...

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Autores principales:	O. V. Gromova, I. A. Kuz'michenko, M. N. Kireev, S. A. Nizhegorodtsev, V. N. Korsukov
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2012
Materias:	cholera vaccine enzymes proteovibrin ultrafiltration Science Q
Acceso en línea:	https://doaj.org/article/47b70880f9d74b3c8f5a42574653f95d
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spelling	oai:doaj.org-article:47b70880f9d74b3c8f5a42574653f95d2021-11-23T06:14:23ZNEW WAY OF ISOLATION OF ENZYME COMPLEX OF CHOLERA VIBRIO - PROTEOVIBRIN BY MEANS OF ULTRAFILTRATION2541-94202587-9596https://doaj.org/article/47b70880f9d74b3c8f5a42574653f95d2012-09-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/1127https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596The technology of isolation of the cholera vibrio complex of low-molecular enzymes related to pathogenicity factors, from cholera vaccine production wastes by means of concentrating ultrafiltration was developed. This technology allows to obtain standard series of the preparation termed proteovibrin which is free from the basic Vibrio cholerae immunogen - O-antigen, and possesses high activity of protease and. phospholipases A2 and. C. It is shown that proteovibrin is highly soluble in water and 0.14 molar NaCl solution, causes the production of antibodies in rabbits and is toxic for white mice. Its further examination seems to be promising, as well as its possible use as additional source of the cholera vibrio enzymes and. as tripsin-like preparation, applicable for protein raw material hydrolysis in production of microbiological nutrient media.O. V. GromovaI. A. Kuz'michenkoM. N. KireevS. A. NizhegorodtsevV. N. KorsukovScientific Сentre for Family Health and Human Reproduction Problemsarticlecholera vaccineenzymesproteovibrinultrafiltrationScienceQRUActa Biomedica Scientifica, Vol 0, Iss 5(1), Pp 201-205 (2012)
institution	DOAJ
collection	DOAJ
language	RU
topic	cholera vaccine enzymes proteovibrin ultrafiltration Science Q
spellingShingle	cholera vaccine enzymes proteovibrin ultrafiltration Science Q O. V. Gromova I. A. Kuz'michenko M. N. Kireev S. A. Nizhegorodtsev V. N. Korsukov NEW WAY OF ISOLATION OF ENZYME COMPLEX OF CHOLERA VIBRIO - PROTEOVIBRIN BY MEANS OF ULTRAFILTRATION
description	The technology of isolation of the cholera vibrio complex of low-molecular enzymes related to pathogenicity factors, from cholera vaccine production wastes by means of concentrating ultrafiltration was developed. This technology allows to obtain standard series of the preparation termed proteovibrin which is free from the basic Vibrio cholerae immunogen - O-antigen, and possesses high activity of protease and. phospholipases A2 and. C. It is shown that proteovibrin is highly soluble in water and 0.14 molar NaCl solution, causes the production of antibodies in rabbits and is toxic for white mice. Its further examination seems to be promising, as well as its possible use as additional source of the cholera vibrio enzymes and. as tripsin-like preparation, applicable for protein raw material hydrolysis in production of microbiological nutrient media.
format	article
author	O. V. Gromova I. A. Kuz'michenko M. N. Kireev S. A. Nizhegorodtsev V. N. Korsukov
author_facet	O. V. Gromova I. A. Kuz'michenko M. N. Kireev S. A. Nizhegorodtsev V. N. Korsukov
author_sort	O. V. Gromova
title	NEW WAY OF ISOLATION OF ENZYME COMPLEX OF CHOLERA VIBRIO - PROTEOVIBRIN BY MEANS OF ULTRAFILTRATION
title_short	NEW WAY OF ISOLATION OF ENZYME COMPLEX OF CHOLERA VIBRIO - PROTEOVIBRIN BY MEANS OF ULTRAFILTRATION
title_full	NEW WAY OF ISOLATION OF ENZYME COMPLEX OF CHOLERA VIBRIO - PROTEOVIBRIN BY MEANS OF ULTRAFILTRATION
title_fullStr	NEW WAY OF ISOLATION OF ENZYME COMPLEX OF CHOLERA VIBRIO - PROTEOVIBRIN BY MEANS OF ULTRAFILTRATION
title_full_unstemmed	NEW WAY OF ISOLATION OF ENZYME COMPLEX OF CHOLERA VIBRIO - PROTEOVIBRIN BY MEANS OF ULTRAFILTRATION
title_sort	new way of isolation of enzyme complex of cholera vibrio - proteovibrin by means of ultrafiltration
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2012
url	https://doaj.org/article/47b70880f9d74b3c8f5a42574653f95d
work_keys_str_mv	AT ovgromova newwayofisolationofenzymecomplexofcholeravibrioproteovibrinbymeansofultrafiltration AT iakuzmichenko newwayofisolationofenzymecomplexofcholeravibrioproteovibrinbymeansofultrafiltration AT mnkireev newwayofisolationofenzymecomplexofcholeravibrioproteovibrinbymeansofultrafiltration AT sanizhegorodtsev newwayofisolationofenzymecomplexofcholeravibrioproteovibrinbymeansofultrafiltration AT vnkorsukov newwayofisolationofenzymecomplexofcholeravibrioproteovibrinbymeansofultrafiltration
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NEW WAY OF ISOLATION OF ENZYME COMPLEX OF CHOLERA VIBRIO - PROTEOVIBRIN BY MEANS OF ULTRAFILTRATION

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