Enrichment of leukocytes in peripheral blood using 3D printed tubes.

Leukocytes have an essential role in patient clinical trajectories and progression. Traditional methods of leukocyte enrichment have many significant limitations for current applications. It is demonstrated a novel 3D printing leukocyte sorting accumulator that combines with centrifugation to ensure...

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Autores principales:	Li-Fang Guo, Liu Wang, Sai Ren, Ning Su, Kun Wei, Xian-Ge Sun, Xiao-Dong Ren, Qing Huang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/47b99afb5a074fed8652ae7f7b9316cd
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spelling	oai:doaj.org-article:47b99afb5a074fed8652ae7f7b9316cd2021-12-02T20:04:53ZEnrichment of leukocytes in peripheral blood using 3D printed tubes.1932-620310.1371/journal.pone.0254615https://doaj.org/article/47b99afb5a074fed8652ae7f7b9316cd2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254615https://doaj.org/toc/1932-6203Leukocytes have an essential role in patient clinical trajectories and progression. Traditional methods of leukocyte enrichment have many significant limitations for current applications. It is demonstrated a novel 3D printing leukocyte sorting accumulator that combines with centrifugation to ensure label-free initial leukocyte enrichment based on cell density and size. The internal structure of leukocyte sorting accumulator (revealed here in a new design, leukocyte sorting accumulator-3, upgraded from earlier models), optimizes localization of the buffy coat fraction and the length of the period allocated for a second centrifugation step to deliver a higher recovery of buffy coats than earlier models. Established methodological parameters were evaluated for reliability by calculating leukocyte recovery rates and erythrocyte depletion rates by both pushing and pulling methods of cell displacement. Results indicate that leukocyte sorting accumulator-3 achieves a mean leukocytes recovery fraction of 96.2 ± 2.38% by the pushing method of layer displacement. By the pulling method, the leukocyte sorting accumulator-3 yield a mean leukocytes recovery fraction of 94.4 ± 0.8%. New procedures for preliminary enrichment of leukocytes from peripheral blood that avoid cellular damage, as well as avert metabolic and phase cycle intervention, are required as the first step in many modern clinical and basic research assays.Li-Fang GuoLiu WangSai RenNing SuKun WeiXian-Ge SunXiao-Dong RenQing HuangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254615 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Li-Fang Guo Liu Wang Sai Ren Ning Su Kun Wei Xian-Ge Sun Xiao-Dong Ren Qing Huang Enrichment of leukocytes in peripheral blood using 3D printed tubes.
description	Leukocytes have an essential role in patient clinical trajectories and progression. Traditional methods of leukocyte enrichment have many significant limitations for current applications. It is demonstrated a novel 3D printing leukocyte sorting accumulator that combines with centrifugation to ensure label-free initial leukocyte enrichment based on cell density and size. The internal structure of leukocyte sorting accumulator (revealed here in a new design, leukocyte sorting accumulator-3, upgraded from earlier models), optimizes localization of the buffy coat fraction and the length of the period allocated for a second centrifugation step to deliver a higher recovery of buffy coats than earlier models. Established methodological parameters were evaluated for reliability by calculating leukocyte recovery rates and erythrocyte depletion rates by both pushing and pulling methods of cell displacement. Results indicate that leukocyte sorting accumulator-3 achieves a mean leukocytes recovery fraction of 96.2 ± 2.38% by the pushing method of layer displacement. By the pulling method, the leukocyte sorting accumulator-3 yield a mean leukocytes recovery fraction of 94.4 ± 0.8%. New procedures for preliminary enrichment of leukocytes from peripheral blood that avoid cellular damage, as well as avert metabolic and phase cycle intervention, are required as the first step in many modern clinical and basic research assays.
format	article
author	Li-Fang Guo Liu Wang Sai Ren Ning Su Kun Wei Xian-Ge Sun Xiao-Dong Ren Qing Huang
author_facet	Li-Fang Guo Liu Wang Sai Ren Ning Su Kun Wei Xian-Ge Sun Xiao-Dong Ren Qing Huang
author_sort	Li-Fang Guo
title	Enrichment of leukocytes in peripheral blood using 3D printed tubes.
title_short	Enrichment of leukocytes in peripheral blood using 3D printed tubes.
title_full	Enrichment of leukocytes in peripheral blood using 3D printed tubes.
title_fullStr	Enrichment of leukocytes in peripheral blood using 3D printed tubes.
title_full_unstemmed	Enrichment of leukocytes in peripheral blood using 3D printed tubes.
title_sort	enrichment of leukocytes in peripheral blood using 3d printed tubes.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/47b99afb5a074fed8652ae7f7b9316cd
work_keys_str_mv	AT lifangguo enrichmentofleukocytesinperipheralbloodusing3dprintedtubes AT liuwang enrichmentofleukocytesinperipheralbloodusing3dprintedtubes AT sairen enrichmentofleukocytesinperipheralbloodusing3dprintedtubes AT ningsu enrichmentofleukocytesinperipheralbloodusing3dprintedtubes AT kunwei enrichmentofleukocytesinperipheralbloodusing3dprintedtubes AT xiangesun enrichmentofleukocytesinperipheralbloodusing3dprintedtubes AT xiaodongren enrichmentofleukocytesinperipheralbloodusing3dprintedtubes AT qinghuang enrichmentofleukocytesinperipheralbloodusing3dprintedtubes
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Enrichment of leukocytes in peripheral blood using 3D printed tubes.

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