Over-expression of KdSOC1 gene affected plantlet morphogenesis in Kalanchoe daigremontiana

Abstract Kalanchoe daigremontiana reproduces asexually by producing plantlets along the leaf margin. The aim of this study was to identify the function of the SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 gene in Kalanchoe daigremontiana (KdSOC1) during plantlet morphogenesis. In this study, KdSOC1 gen...

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Autores principales:	Chen Zhu, Li Wang, Jinhua Chen, Chenglan Liu, Huiming Zeng, Huafang Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/47bd0b6ec7a4454390d096658f0ad4a1
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spelling	oai:doaj.org-article:47bd0b6ec7a4454390d096658f0ad4a12021-12-02T11:51:11ZOver-expression of KdSOC1 gene affected plantlet morphogenesis in Kalanchoe daigremontiana10.1038/s41598-017-04387-02045-2322https://doaj.org/article/47bd0b6ec7a4454390d096658f0ad4a12017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04387-0https://doaj.org/toc/2045-2322Abstract Kalanchoe daigremontiana reproduces asexually by producing plantlets along the leaf margin. The aim of this study was to identify the function of the SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 gene in Kalanchoe daigremontiana (KdSOC1) during plantlet morphogenesis. In this study, KdSOC1 gene expression was detected at stem cell niche during in vitro somatic embryogenesis and plantlet morphogenesis. Disrupting endogenous auxin transportation suppressed the KdSOC1 gene response. Knockdown of the KdSOC1 gene caused a defect in cotyledon formation during the early heart stage of somatic embryogenesis. Over-expression (OE) of the KdSOC1 gene resulted in asymmetric plantlet distribution, a reduced number of plantlets, thicker leaves, and thicker vascular fibers. Higher KdPIN1 gene expression and auxin content were found in OE plant compared to those of wild-type plant leaves, which indicated possible KdSOC1 gene role in affecting auxin distribution and accumulation. KdSOC1 gene OE in DR5-GUS Arabidopsis reporting lines resulted in an abnormal auxin response pattern during different stages of somatic embryogenesis. In summary, the KdSOC1 gene OE might alter auxin distribution and accumulation along leaf margin to initiate plantlet formation and distribution, which is crucial for plasticity during plantlet formation under various environmental conditions.Chen ZhuLi WangJinhua ChenChenglan LiuHuiming ZengHuafang WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chen Zhu Li Wang Jinhua Chen Chenglan Liu Huiming Zeng Huafang Wang Over-expression of KdSOC1 gene affected plantlet morphogenesis in Kalanchoe daigremontiana
description	Abstract Kalanchoe daigremontiana reproduces asexually by producing plantlets along the leaf margin. The aim of this study was to identify the function of the SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 gene in Kalanchoe daigremontiana (KdSOC1) during plantlet morphogenesis. In this study, KdSOC1 gene expression was detected at stem cell niche during in vitro somatic embryogenesis and plantlet morphogenesis. Disrupting endogenous auxin transportation suppressed the KdSOC1 gene response. Knockdown of the KdSOC1 gene caused a defect in cotyledon formation during the early heart stage of somatic embryogenesis. Over-expression (OE) of the KdSOC1 gene resulted in asymmetric plantlet distribution, a reduced number of plantlets, thicker leaves, and thicker vascular fibers. Higher KdPIN1 gene expression and auxin content were found in OE plant compared to those of wild-type plant leaves, which indicated possible KdSOC1 gene role in affecting auxin distribution and accumulation. KdSOC1 gene OE in DR5-GUS Arabidopsis reporting lines resulted in an abnormal auxin response pattern during different stages of somatic embryogenesis. In summary, the KdSOC1 gene OE might alter auxin distribution and accumulation along leaf margin to initiate plantlet formation and distribution, which is crucial for plasticity during plantlet formation under various environmental conditions.
format	article
author	Chen Zhu Li Wang Jinhua Chen Chenglan Liu Huiming Zeng Huafang Wang
author_facet	Chen Zhu Li Wang Jinhua Chen Chenglan Liu Huiming Zeng Huafang Wang
author_sort	Chen Zhu
title	Over-expression of KdSOC1 gene affected plantlet morphogenesis in Kalanchoe daigremontiana
title_short	Over-expression of KdSOC1 gene affected plantlet morphogenesis in Kalanchoe daigremontiana
title_full	Over-expression of KdSOC1 gene affected plantlet morphogenesis in Kalanchoe daigremontiana
title_fullStr	Over-expression of KdSOC1 gene affected plantlet morphogenesis in Kalanchoe daigremontiana
title_full_unstemmed	Over-expression of KdSOC1 gene affected plantlet morphogenesis in Kalanchoe daigremontiana
title_sort	over-expression of kdsoc1 gene affected plantlet morphogenesis in kalanchoe daigremontiana
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/47bd0b6ec7a4454390d096658f0ad4a1
work_keys_str_mv	AT chenzhu overexpressionofkdsoc1geneaffectedplantletmorphogenesisinkalanchoedaigremontiana AT liwang overexpressionofkdsoc1geneaffectedplantletmorphogenesisinkalanchoedaigremontiana AT jinhuachen overexpressionofkdsoc1geneaffectedplantletmorphogenesisinkalanchoedaigremontiana AT chenglanliu overexpressionofkdsoc1geneaffectedplantletmorphogenesisinkalanchoedaigremontiana AT huimingzeng overexpressionofkdsoc1geneaffectedplantletmorphogenesisinkalanchoedaigremontiana AT huafangwang overexpressionofkdsoc1geneaffectedplantletmorphogenesisinkalanchoedaigremontiana
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Over-expression of KdSOC1 gene affected plantlet morphogenesis in Kalanchoe daigremontiana

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