Borrelia valaisiana resist complement-mediated killing independently of the recruitment of immune regulators and inactivation of complement components.

Spirochetes belonging to the Borrelia (B.) burgdorferi sensu lato complex differ in their resistance to complement-mediated killing, particularly in regard to human serum. In the present study, we elucidate the serum and complement susceptibility of B. valaisiana, a genospecies with the potential to...

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Autores principales:	Jasmin Schwab, Claudia Hammerschmidt, Dania Richter, Christine Skerka, Franz-Rainer Matuschka, Reinhard Wallich, Peter F Zipfel, Peter Kraiczy
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/47cc9e4df0a44d48875f737aad721b03
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spelling	oai:doaj.org-article:47cc9e4df0a44d48875f737aad721b032021-11-18T08:02:18ZBorrelia valaisiana resist complement-mediated killing independently of the recruitment of immune regulators and inactivation of complement components.1932-620310.1371/journal.pone.0053659https://doaj.org/article/47cc9e4df0a44d48875f737aad721b032013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23320099/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Spirochetes belonging to the Borrelia (B.) burgdorferi sensu lato complex differ in their resistance to complement-mediated killing, particularly in regard to human serum. In the present study, we elucidate the serum and complement susceptibility of B. valaisiana, a genospecies with the potential to cause Lyme disease in Europe as well as in Asia. Among the investigated isolates, growth of ZWU3 Ny3 was not affected while growth of VS116 and Bv9 was strongly inhibited in the presence of 50% human serum. Analyzing complement activation, complement components C3, C4 and C6 were deposited on the surface of isolates VS116 and Bv9, and similarly the membrane attack complex was formed on their surface. In contrast, no surface-deposited components and no aberrations in cell morphology were detected for serum-resistant ZWU3 Ny3. While further investigating the protective role of bound complement regulators in mediating complement resistance, we discovered that none of the B. valaisiana isolates analyzed bound complement regulators Factor H, Factor H-like protein 1, C4b binding protein or C1 esterase inhibitor. In addition, B. valaisiana also lacked intrinsic proteolytic activity to degrade complement components C3, C3b, C4, C4b, and C5. Taken together, these findings suggest that certain B. valaisiana isolates differ in their capability to resist complement-mediating killing by human serum. The molecular mechanism utilized by B. valaisiana to inhibit bacteriolysis appears not to involve binding of the key host complement regulators of the alternative, classical, and lectin pathways as already known for serum-resistant Lyme disease or relapsing fever borreliae.Jasmin SchwabClaudia HammerschmidtDania RichterChristine SkerkaFranz-Rainer MatuschkaReinhard WallichPeter F ZipfelPeter KraiczyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e53659 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jasmin Schwab Claudia Hammerschmidt Dania Richter Christine Skerka Franz-Rainer Matuschka Reinhard Wallich Peter F Zipfel Peter Kraiczy Borrelia valaisiana resist complement-mediated killing independently of the recruitment of immune regulators and inactivation of complement components.
description	Spirochetes belonging to the Borrelia (B.) burgdorferi sensu lato complex differ in their resistance to complement-mediated killing, particularly in regard to human serum. In the present study, we elucidate the serum and complement susceptibility of B. valaisiana, a genospecies with the potential to cause Lyme disease in Europe as well as in Asia. Among the investigated isolates, growth of ZWU3 Ny3 was not affected while growth of VS116 and Bv9 was strongly inhibited in the presence of 50% human serum. Analyzing complement activation, complement components C3, C4 and C6 were deposited on the surface of isolates VS116 and Bv9, and similarly the membrane attack complex was formed on their surface. In contrast, no surface-deposited components and no aberrations in cell morphology were detected for serum-resistant ZWU3 Ny3. While further investigating the protective role of bound complement regulators in mediating complement resistance, we discovered that none of the B. valaisiana isolates analyzed bound complement regulators Factor H, Factor H-like protein 1, C4b binding protein or C1 esterase inhibitor. In addition, B. valaisiana also lacked intrinsic proteolytic activity to degrade complement components C3, C3b, C4, C4b, and C5. Taken together, these findings suggest that certain B. valaisiana isolates differ in their capability to resist complement-mediating killing by human serum. The molecular mechanism utilized by B. valaisiana to inhibit bacteriolysis appears not to involve binding of the key host complement regulators of the alternative, classical, and lectin pathways as already known for serum-resistant Lyme disease or relapsing fever borreliae.
format	article
author	Jasmin Schwab Claudia Hammerschmidt Dania Richter Christine Skerka Franz-Rainer Matuschka Reinhard Wallich Peter F Zipfel Peter Kraiczy
author_facet	Jasmin Schwab Claudia Hammerschmidt Dania Richter Christine Skerka Franz-Rainer Matuschka Reinhard Wallich Peter F Zipfel Peter Kraiczy
author_sort	Jasmin Schwab
title	Borrelia valaisiana resist complement-mediated killing independently of the recruitment of immune regulators and inactivation of complement components.
title_short	Borrelia valaisiana resist complement-mediated killing independently of the recruitment of immune regulators and inactivation of complement components.
title_full	Borrelia valaisiana resist complement-mediated killing independently of the recruitment of immune regulators and inactivation of complement components.
title_fullStr	Borrelia valaisiana resist complement-mediated killing independently of the recruitment of immune regulators and inactivation of complement components.
title_full_unstemmed	Borrelia valaisiana resist complement-mediated killing independently of the recruitment of immune regulators and inactivation of complement components.
title_sort	borrelia valaisiana resist complement-mediated killing independently of the recruitment of immune regulators and inactivation of complement components.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/47cc9e4df0a44d48875f737aad721b03
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Borrelia valaisiana resist complement-mediated killing independently of the recruitment of immune regulators and inactivation of complement components.

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