Long-lasting bactericidal activity through selective physical puncture and controlled ions release of polydopamine and silver nanoparticles–loaded TiO2 nanorods in vitro and in vivo
Ming Guan,1,2 Yangmengfan Chen,1 Yong Wei,3 Hao Song,3 Chenghao Gao,1 Hao Cheng,4 Yong Li,1 Kaifu Huo,5 Jijiang Fu,3 Wei Xiong11Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People’s Republic of China; 2Depa...
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Dove Medical Press
2019
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oai:doaj.org-article:47d03c1be1734f8a9a463222f68a9f462021-12-02T05:40:31ZLong-lasting bactericidal activity through selective physical puncture and controlled ions release of polydopamine and silver nanoparticles–loaded TiO2 nanorods in vitro and in vivo1178-2013https://doaj.org/article/47d03c1be1734f8a9a463222f68a9f462019-04-01T00:00:00Zhttps://www.dovepress.com/long-lasting-bactericidal-activity-through-selective-physical-puncture-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ming Guan,1,2 Yangmengfan Chen,1 Yong Wei,3 Hao Song,3 Chenghao Gao,1 Hao Cheng,4 Yong Li,1 Kaifu Huo,5 Jijiang Fu,3 Wei Xiong11Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People’s Republic of China; 2Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109-1078, USA; 3The State Key Laboratory of Refractories and Metallurgy, Wuhan University of Science and Technology, Wuhan 430081, People’s Republic of China; 4Department of Orthopedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, People’s Republic of China; 5Wuhan National Lab for Optoelectronics, Huazhong University of Science and Techonology, Wuhan 430074, People’s Republic of ChinaBackground: Titanium (Ti) implant-associated infection, which is mostly caused by bacterial adhesion and biofilm formation, may result in implant failure and secondary surgery. Thus it is an urgent issue to prevent bacterial infections at the earliest step.Purpose: To develop a novel surface strategy of polydopamine (PDA) and silver (Ag) nanoparticle-loaded TiO2 nanorods (NRDs) coatings on Ti alloy.Materials and methods: Ag-TiO2@PDA NRDs was fabricated on Ti alloy by hydrothermal synthesis. The antibacterial activity of Ag-TiO2@PDA NRDs against Escherichia coli and methicillin-resistant Staphylococcus aureus were tested by FE-SEM, Live/Dead staining, zone of inhibition, bacteria counting method and protein leakage analysis in vitro. In addition, an implant infection model was conducted and the samples were tested by X-ray, Micro-CT and histological analysis in vivo. Besides, cell morphology and cytotoxicity of Mouse calvarial cells (MC3T3-E1) were characterized by FE-SEM, immunofluorescence and CCK-8 test in vitro.Results: Our study successfully developed a new surface coating of Ag-TiO2@PDA NRDs. The selective physical puncture of bacteria and controlled release of Ag+ ions of Ag-TiO2@PDA NRDs achieved a long-lasting bactericidal ability and anti-biofilm activity with satisfied biocompatibility.Conclusion: This strategy may be promising for clinical applications to reduce the occurrence of infection in the implant surgeriesKeywords: bacterial infection, polydopamine, silver nanoparticles, physical puncture, TiO2 nanorods, bactericidal activityGuan MChen YWei YSong HGao CCheng HLi YHuo KFu JXiong WDove Medical PressarticleBacterial infectionpolydopaminesilver nanoparticlesphysical punctureTiO2 nanorodsbactericidal activityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 2903-2914 (2019) |
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Bacterial infection polydopamine silver nanoparticles physical puncture TiO2 nanorods bactericidal activity Medicine (General) R5-920 |
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Bacterial infection polydopamine silver nanoparticles physical puncture TiO2 nanorods bactericidal activity Medicine (General) R5-920 Guan M Chen Y Wei Y Song H Gao C Cheng H Li Y Huo K Fu J Xiong W Long-lasting bactericidal activity through selective physical puncture and controlled ions release of polydopamine and silver nanoparticles–loaded TiO2 nanorods in vitro and in vivo |
| description |
Ming Guan,1,2 Yangmengfan Chen,1 Yong Wei,3 Hao Song,3 Chenghao Gao,1 Hao Cheng,4 Yong Li,1 Kaifu Huo,5 Jijiang Fu,3 Wei Xiong11Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People’s Republic of China; 2Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109-1078, USA; 3The State Key Laboratory of Refractories and Metallurgy, Wuhan University of Science and Technology, Wuhan 430081, People’s Republic of China; 4Department of Orthopedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, People’s Republic of China; 5Wuhan National Lab for Optoelectronics, Huazhong University of Science and Techonology, Wuhan 430074, People’s Republic of ChinaBackground: Titanium (Ti) implant-associated infection, which is mostly caused by bacterial adhesion and biofilm formation, may result in implant failure and secondary surgery. Thus it is an urgent issue to prevent bacterial infections at the earliest step.Purpose: To develop a novel surface strategy of polydopamine (PDA) and silver (Ag) nanoparticle-loaded TiO2 nanorods (NRDs) coatings on Ti alloy.Materials and methods: Ag-TiO2@PDA NRDs was fabricated on Ti alloy by hydrothermal synthesis. The antibacterial activity of Ag-TiO2@PDA NRDs against Escherichia coli and methicillin-resistant Staphylococcus aureus were tested by FE-SEM, Live/Dead staining, zone of inhibition, bacteria counting method and protein leakage analysis in vitro. In addition, an implant infection model was conducted and the samples were tested by X-ray, Micro-CT and histological analysis in vivo. Besides, cell morphology and cytotoxicity of Mouse calvarial cells (MC3T3-E1) were characterized by FE-SEM, immunofluorescence and CCK-8 test in vitro.Results: Our study successfully developed a new surface coating of Ag-TiO2@PDA NRDs. The selective physical puncture of bacteria and controlled release of Ag+ ions of Ag-TiO2@PDA NRDs achieved a long-lasting bactericidal ability and anti-biofilm activity with satisfied biocompatibility.Conclusion: This strategy may be promising for clinical applications to reduce the occurrence of infection in the implant surgeriesKeywords: bacterial infection, polydopamine, silver nanoparticles, physical puncture, TiO2 nanorods, bactericidal activity |
| format |
article |
| author |
Guan M Chen Y Wei Y Song H Gao C Cheng H Li Y Huo K Fu J Xiong W |
| author_facet |
Guan M Chen Y Wei Y Song H Gao C Cheng H Li Y Huo K Fu J Xiong W |
| author_sort |
Guan M |
| title |
Long-lasting bactericidal activity through selective physical puncture and controlled ions release of polydopamine and silver nanoparticles–loaded TiO2 nanorods in vitro and in vivo |
| title_short |
Long-lasting bactericidal activity through selective physical puncture and controlled ions release of polydopamine and silver nanoparticles–loaded TiO2 nanorods in vitro and in vivo |
| title_full |
Long-lasting bactericidal activity through selective physical puncture and controlled ions release of polydopamine and silver nanoparticles–loaded TiO2 nanorods in vitro and in vivo |
| title_fullStr |
Long-lasting bactericidal activity through selective physical puncture and controlled ions release of polydopamine and silver nanoparticles–loaded TiO2 nanorods in vitro and in vivo |
| title_full_unstemmed |
Long-lasting bactericidal activity through selective physical puncture and controlled ions release of polydopamine and silver nanoparticles–loaded TiO2 nanorods in vitro and in vivo |
| title_sort |
long-lasting bactericidal activity through selective physical puncture and controlled ions release of polydopamine and silver nanoparticles–loaded tio2 nanorods in vitro and in vivo |
| publisher |
Dove Medical Press |
| publishDate |
2019 |
| url |
https://doaj.org/article/47d03c1be1734f8a9a463222f68a9f46 |
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