AGO1 and AGO2 act redundantly in miR408-mediated Plantacyanin regulation.

<h4>Background</h4>In Arabidopsis, AGO1 and AGO2 associate with small RNAs that exhibit a Uridine and an Adenosine at their 5' end, respectively. Because most plant miRNAs have a 5'U, AGO1 plays many essential roles in miRNA-mediated regulation of development and stress respons...

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Autores principales:	Nicolas Maunoury, Hervé Vaucheret
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/47d3ff4ce5174f51821182ae53c963fb
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spelling	oai:doaj.org-article:47d3ff4ce5174f51821182ae53c963fb2021-11-18T07:32:27ZAGO1 and AGO2 act redundantly in miR408-mediated Plantacyanin regulation.1932-620310.1371/journal.pone.0028729https://doaj.org/article/47d3ff4ce5174f51821182ae53c963fb2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22174881/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>In Arabidopsis, AGO1 and AGO2 associate with small RNAs that exhibit a Uridine and an Adenosine at their 5' end, respectively. Because most plant miRNAs have a 5'U, AGO1 plays many essential roles in miRNA-mediated regulation of development and stress responses. In contrast, AGO2 has only been implicated in antibacterial defense in association with miR393*, which has a 5'A. AGO2 also participates in antiviral defense in association with viral siRNAs.<h4>Principal findings</h4>This study reveals that miR408, which has a 5'A, regulates its target Plantacyanin through either AGO1 or AGO2. Indeed, neither ago1 nor ago2 single mutations abolish miR408-mediated regulation of Plantacyanin. Only an ago1 ago2 double mutant appears compromised in miR408-mediated regulation of Plantacyanin, suggesting that AGO1 and AGO2 have redundant roles in this regulation. Moreover, the nature of the 5' nucleotide of miR408 does not appear essential for its regulatory role because both a wildtype 5'A-MIR408 and a mutant 5'U-MIR408 gene complement a mir408 mutant.<h4>Conclusions/significance</h4>These results suggest that miR408 associates with both AGO1 and AGO2 based on criteria that differ from the 5' end rule, reminiscent of miR390-AGO7 and miR165/166-AGO10 associations, which are not based on the nature of the 5' nucleotide.Nicolas MaunouryHervé VaucheretPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e28729 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nicolas Maunoury Hervé Vaucheret AGO1 and AGO2 act redundantly in miR408-mediated Plantacyanin regulation.
description	<h4>Background</h4>In Arabidopsis, AGO1 and AGO2 associate with small RNAs that exhibit a Uridine and an Adenosine at their 5' end, respectively. Because most plant miRNAs have a 5'U, AGO1 plays many essential roles in miRNA-mediated regulation of development and stress responses. In contrast, AGO2 has only been implicated in antibacterial defense in association with miR393*, which has a 5'A. AGO2 also participates in antiviral defense in association with viral siRNAs.<h4>Principal findings</h4>This study reveals that miR408, which has a 5'A, regulates its target Plantacyanin through either AGO1 or AGO2. Indeed, neither ago1 nor ago2 single mutations abolish miR408-mediated regulation of Plantacyanin. Only an ago1 ago2 double mutant appears compromised in miR408-mediated regulation of Plantacyanin, suggesting that AGO1 and AGO2 have redundant roles in this regulation. Moreover, the nature of the 5' nucleotide of miR408 does not appear essential for its regulatory role because both a wildtype 5'A-MIR408 and a mutant 5'U-MIR408 gene complement a mir408 mutant.<h4>Conclusions/significance</h4>These results suggest that miR408 associates with both AGO1 and AGO2 based on criteria that differ from the 5' end rule, reminiscent of miR390-AGO7 and miR165/166-AGO10 associations, which are not based on the nature of the 5' nucleotide.
format	article
author	Nicolas Maunoury Hervé Vaucheret
author_facet	Nicolas Maunoury Hervé Vaucheret
author_sort	Nicolas Maunoury
title	AGO1 and AGO2 act redundantly in miR408-mediated Plantacyanin regulation.
title_short	AGO1 and AGO2 act redundantly in miR408-mediated Plantacyanin regulation.
title_full	AGO1 and AGO2 act redundantly in miR408-mediated Plantacyanin regulation.
title_fullStr	AGO1 and AGO2 act redundantly in miR408-mediated Plantacyanin regulation.
title_full_unstemmed	AGO1 and AGO2 act redundantly in miR408-mediated Plantacyanin regulation.
title_sort	ago1 and ago2 act redundantly in mir408-mediated plantacyanin regulation.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/47d3ff4ce5174f51821182ae53c963fb
work_keys_str_mv	AT nicolasmaunoury ago1andago2actredundantlyinmir408mediatedplantacyaninregulation AT hervevaucheret ago1andago2actredundantlyinmir408mediatedplantacyaninregulation
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AGO1 and AGO2 act redundantly in miR408-mediated Plantacyanin regulation.

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