Temperature and cholera toxin B are factors that influence formation of membrane nanotubes in RT4 and T24 urothelial cancer cell lines

Doron Kabaso1*, Maru&scaron;a Lokar1*, Veronika Kralj-Iglic2, Peter Veranic3, Ale&scaron; Iglic11Laboratory of Biophysics, Faculty of Electrical Engineering, 2Laboratory of Clinical Biophysics, Faculty of Medicine, 3Institute of Cell Biology, Faculty of Medicine, University of Ljublj...

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Autores principales:	Doron Kabaso, Maru&scaron;a Lokar, Veronika Kralj-Iglic, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/47d4444ccd444ff7bc4abd9fde16a92d
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spelling	oai:doaj.org-article:47d4444ccd444ff7bc4abd9fde16a92d2021-12-02T02:42:33ZTemperature and cholera toxin B are factors that influence formation of membrane nanotubes in RT4 and T24 urothelial cancer cell lines1176-91141178-2013https://doaj.org/article/47d4444ccd444ff7bc4abd9fde16a92d2011-03-01T00:00:00Zhttp://www.dovepress.com/temperature-and-cholera-toxin-b-are-factors-that-influence-formation-o-a6677https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Doron Kabaso1, Maru&scaron;a Lokar1, Veronika Kralj-Iglic2, Peter Veranic3, Ale&scaron; Iglic11Laboratory of Biophysics, Faculty of Electrical Engineering, 2Laboratory of Clinical Biophysics, Faculty of Medicine, 3Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia; *These two authors equally share the first authorshipAbstract: The growth of membrane nanotubes is crucial for intercellular communication in both normal development and pathological conditions. Therefore, identifying factors that influence their stability and formation are important for both basic research and in development of potential treatments of pathological states. Here we investigate the effect of cholera toxin B (CTB) and temperature on two pathological model systems: urothelial cell line RT4, as a model system of a benign tumor, and urothelial cell line T24, as a model system of a metastatic tumor. In particular, the number of intercellular membrane nanotubes (ICNs; ie, membrane nanotubes that bridge neighboring cells) was counted. In comparison with RT4 cells, we reveal a significantly higher number in the density of ICNs in T24 cells not derived from RT4 without treatments (P = 0.005), after 20 minutes at room temperature (P = 0.0007), and following CTB treatment (P = 0.000025). The binding of CTB to GM1&ndash;lipid complexes in membrane exvaginations or tips of membrane nanotubes may reduce the positive spontaneous (intrinsic) curvature of GM1&ndash;lipid complexes, which may lead to lipid mediated attractive interactions between CTB&ndash;GM1&ndash;lipid complexes, their aggregation and consequent formation of enlarged spherical tips of nanotubes. The binding of CTB to GM1 molecules in the outer membrane leaflet of membrane exvaginations and tips of membrane nanotubes may also increase the area difference between the two leaflets and in this way facilitate the growth of membrane nanotubes.Keywords: cancer cells, membrane nanotubes, cholera toxin Doron KabasoMaru&scaron;a LokarVeronika Kralj-Iglicet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 495-509 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Doron Kabaso Maru&scaron;a Lokar Veronika Kralj-Iglic et al Temperature and cholera toxin B are factors that influence formation of membrane nanotubes in RT4 and T24 urothelial cancer cell lines
description	Doron Kabaso1, Maru&scaron;a Lokar1, Veronika Kralj-Iglic2, Peter Veranic3, Ale&scaron; Iglic11Laboratory of Biophysics, Faculty of Electrical Engineering, 2Laboratory of Clinical Biophysics, Faculty of Medicine, 3Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia; *These two authors equally share the first authorshipAbstract: The growth of membrane nanotubes is crucial for intercellular communication in both normal development and pathological conditions. Therefore, identifying factors that influence their stability and formation are important for both basic research and in development of potential treatments of pathological states. Here we investigate the effect of cholera toxin B (CTB) and temperature on two pathological model systems: urothelial cell line RT4, as a model system of a benign tumor, and urothelial cell line T24, as a model system of a metastatic tumor. In particular, the number of intercellular membrane nanotubes (ICNs; ie, membrane nanotubes that bridge neighboring cells) was counted. In comparison with RT4 cells, we reveal a significantly higher number in the density of ICNs in T24 cells not derived from RT4 without treatments (P = 0.005), after 20 minutes at room temperature (P = 0.0007), and following CTB treatment (P = 0.000025). The binding of CTB to GM1&ndash;lipid complexes in membrane exvaginations or tips of membrane nanotubes may reduce the positive spontaneous (intrinsic) curvature of GM1&ndash;lipid complexes, which may lead to lipid mediated attractive interactions between CTB&ndash;GM1&ndash;lipid complexes, their aggregation and consequent formation of enlarged spherical tips of nanotubes. The binding of CTB to GM1 molecules in the outer membrane leaflet of membrane exvaginations and tips of membrane nanotubes may also increase the area difference between the two leaflets and in this way facilitate the growth of membrane nanotubes.Keywords: cancer cells, membrane nanotubes, cholera toxin
format	article
author	Doron Kabaso Maru&scaron;a Lokar Veronika Kralj-Iglic et al
author_facet	Doron Kabaso Maru&scaron;a Lokar Veronika Kralj-Iglic et al
author_sort	Doron Kabaso
title	Temperature and cholera toxin B are factors that influence formation of membrane nanotubes in RT4 and T24 urothelial cancer cell lines
title_short	Temperature and cholera toxin B are factors that influence formation of membrane nanotubes in RT4 and T24 urothelial cancer cell lines
title_full	Temperature and cholera toxin B are factors that influence formation of membrane nanotubes in RT4 and T24 urothelial cancer cell lines
title_fullStr	Temperature and cholera toxin B are factors that influence formation of membrane nanotubes in RT4 and T24 urothelial cancer cell lines
title_full_unstemmed	Temperature and cholera toxin B are factors that influence formation of membrane nanotubes in RT4 and T24 urothelial cancer cell lines
title_sort	temperature and cholera toxin b are factors that influence formation of membrane nanotubes in rt4 and t24 urothelial cancer cell lines
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/47d4444ccd444ff7bc4abd9fde16a92d
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_version_	1718402214794362880

Temperature and cholera toxin B are factors that influence formation of membrane nanotubes in RT4 and T24 urothelial cancer cell lines

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