Selectivity of direct plasma treatment and plasma-conditioned media in bone cancer cell lines

Abstract Atmospheric pressure plasma jets have been shown to impact several cancer cell lines, both in vitro and in vivo. These effects are based on the biochemistry of the reactive oxygen and nitrogen species generated by plasmas in physiological liquids, referred to as plasma-conditioned liquids....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Inès Hamouda, Cédric Labay, Uroš Cvelbar, Maria-Pau Ginebra, Cristina Canal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/47d921fd105545d185e32ea419c02b27
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:47d921fd105545d185e32ea419c02b27
record_format	dspace
spelling	oai:doaj.org-article:47d921fd105545d185e32ea419c02b272021-12-02T15:28:47ZSelectivity of direct plasma treatment and plasma-conditioned media in bone cancer cell lines10.1038/s41598-021-96857-92045-2322https://doaj.org/article/47d921fd105545d185e32ea419c02b272021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96857-9https://doaj.org/toc/2045-2322Abstract Atmospheric pressure plasma jets have been shown to impact several cancer cell lines, both in vitro and in vivo. These effects are based on the biochemistry of the reactive oxygen and nitrogen species generated by plasmas in physiological liquids, referred to as plasma-conditioned liquids. Plasma-conditioned media are efficient in the generation of reactive species, inducing selective cancer cell death. However, the concentration of reactive species generated by plasma in the cell culture media of different cell types can be highly variable, complicating the ability to draw precise conclusions due to the differential sensitivity of different cells to reactive species. Here, we compared the effects of direct and indirect plasma treatment on non-malignant bone cells (hOBs and hMSCs) and bone cancer cells (SaOs-2s and MG63s) by treating the cells directly or exposing them to previously treated cell culture medium. Biological effects were correlated with the concentrations of reactive species generated in the liquid. A linear increase in reactive species in the cell culture medium was observed with increased plasma treatment time independent of the volume treated. Values up to 700 µM for H2O2 and 140 µM of NO2 − were attained in 2 mL after 15 min of plasma treatment in AdvDMEM cell culture media. Selectivity towards bone cancer cells was observed after both direct and indirect plasma treatments, leading to a decrease in bone cancer cell viability at 72 h to 30% for the longest plasma treatment times while maintaining the survival of non-malignant cells. Therefore, plasma-conditioned media may represent the basis for a potentially novel non-invasive technique for bone cancer therapy.Inès HamoudaCédric LabayUroš CvelbarMaria-Pau GinebraCristina CanalNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Inès Hamouda Cédric Labay Uroš Cvelbar Maria-Pau Ginebra Cristina Canal Selectivity of direct plasma treatment and plasma-conditioned media in bone cancer cell lines
description	Abstract Atmospheric pressure plasma jets have been shown to impact several cancer cell lines, both in vitro and in vivo. These effects are based on the biochemistry of the reactive oxygen and nitrogen species generated by plasmas in physiological liquids, referred to as plasma-conditioned liquids. Plasma-conditioned media are efficient in the generation of reactive species, inducing selective cancer cell death. However, the concentration of reactive species generated by plasma in the cell culture media of different cell types can be highly variable, complicating the ability to draw precise conclusions due to the differential sensitivity of different cells to reactive species. Here, we compared the effects of direct and indirect plasma treatment on non-malignant bone cells (hOBs and hMSCs) and bone cancer cells (SaOs-2s and MG63s) by treating the cells directly or exposing them to previously treated cell culture medium. Biological effects were correlated with the concentrations of reactive species generated in the liquid. A linear increase in reactive species in the cell culture medium was observed with increased plasma treatment time independent of the volume treated. Values up to 700 µM for H2O2 and 140 µM of NO2 − were attained in 2 mL after 15 min of plasma treatment in AdvDMEM cell culture media. Selectivity towards bone cancer cells was observed after both direct and indirect plasma treatments, leading to a decrease in bone cancer cell viability at 72 h to 30% for the longest plasma treatment times while maintaining the survival of non-malignant cells. Therefore, plasma-conditioned media may represent the basis for a potentially novel non-invasive technique for bone cancer therapy.
format	article
author	Inès Hamouda Cédric Labay Uroš Cvelbar Maria-Pau Ginebra Cristina Canal
author_facet	Inès Hamouda Cédric Labay Uroš Cvelbar Maria-Pau Ginebra Cristina Canal
author_sort	Inès Hamouda
title	Selectivity of direct plasma treatment and plasma-conditioned media in bone cancer cell lines
title_short	Selectivity of direct plasma treatment and plasma-conditioned media in bone cancer cell lines
title_full	Selectivity of direct plasma treatment and plasma-conditioned media in bone cancer cell lines
title_fullStr	Selectivity of direct plasma treatment and plasma-conditioned media in bone cancer cell lines
title_full_unstemmed	Selectivity of direct plasma treatment and plasma-conditioned media in bone cancer cell lines
title_sort	selectivity of direct plasma treatment and plasma-conditioned media in bone cancer cell lines
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/47d921fd105545d185e32ea419c02b27
work_keys_str_mv	AT ineshamouda selectivityofdirectplasmatreatmentandplasmaconditionedmediainbonecancercelllines AT cedriclabay selectivityofdirectplasmatreatmentandplasmaconditionedmediainbonecancercelllines AT uroscvelbar selectivityofdirectplasmatreatmentandplasmaconditionedmediainbonecancercelllines AT mariapauginebra selectivityofdirectplasmatreatmentandplasmaconditionedmediainbonecancercelllines AT cristinacanal selectivityofdirectplasmatreatmentandplasmaconditionedmediainbonecancercelllines
_version_	1718387201295777792

Selectivity of direct plasma treatment and plasma-conditioned media in bone cancer cell lines

Ejemplares similares