Metabolic profile and skin-related bioactivities of Cerioporus squamosus hydromethanolic extract

Abstract. Elkhateeb WA, Daba GM, Elnahas MO, Thomas PW, Emam M. 2020. Metabolic profile and skin-related bioactivities of Cerioporus squamosus hydromethanolic extract. Biodiversitas 21: 4732-4740. Being a functional food capable of showing nutritional as well as medicinal properties have great atte...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Waill Elkhateeb, Ghoson Daba, Marwa Elnahas, Paul Thomas, Mahmoud Emam
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	cerioporus squamosus, edible mushroom, gc- ms, silylated metabolites, skin cancer, wound healing. Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/47e266075c3249d88386bc9dfa7cc19d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:47e266075c3249d88386bc9dfa7cc19d
record_format	dspace
spelling	oai:doaj.org-article:47e266075c3249d88386bc9dfa7cc19d2021-11-22T00:51:09ZMetabolic profile and skin-related bioactivities of Cerioporus squamosus hydromethanolic extract1412-033X2085-472210.13057/biodiv/d211037https://doaj.org/article/47e266075c3249d88386bc9dfa7cc19d2020-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6388https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Elkhateeb WA, Daba GM, Elnahas MO, Thomas PW, Emam M. 2020. Metabolic profile and skin-related bioactivities of Cerioporus squamosus hydromethanolic extract. Biodiversitas 21: 4732-4740. Being a functional food capable of showing nutritional as well as medicinal properties have great attention. Mushrooms have been proven as leading targets in this field. For this purpose, the edible mushroom Cerioporus squamosus was investigated in this study to evaluate the in vitro skin-related bioactivities of its hydromethanolic extract in terms of enhancing wound healing, and human skin cancer suppression capabilities. Treatment of fibroblast cells (BJ-1) with the hydromethanolic extract of this mushroom at 50 µg/mL enhanced cell migration rates by 71.7% after 24 h of exposure to the extract. Moreover, the same extract exhibited a promising impact on human skin cancer using an epidermoid carcinoma cell line (A431). The gradual increase in C. squamosus hydromethanolic extract concentration caused gradual decrease in the A431 cell viability and proliferation. Maximum effect on reducing the cell viability was obtained at a concentration of 100 µg/mL, where cell viability was 3.7%, and recorded IC50 was 52.6 µg/mL. The metabolic profile of the extract was analyzed by GC-MS, which was performed on its silylated metabolites. Nineteen compounds were detected including sugar alcohols, amino acids, fatty and organic acids. Promising results of this mushroom extract encourage conducting further steps towards using this mushroom as a functional food showing promising bioactivities.Waill ElkhateebGhoson DabaMarwa ElnahasPaul ThomasMahmoud EmamMBI & UNS Soloarticlecerioporus squamosus, edible mushroom, gc- ms, silylated metabolites, skin cancer, wound healing.Biology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	cerioporus squamosus, edible mushroom, gc- ms, silylated metabolites, skin cancer, wound healing. Biology (General) QH301-705.5
spellingShingle	cerioporus squamosus, edible mushroom, gc- ms, silylated metabolites, skin cancer, wound healing. Biology (General) QH301-705.5 Waill Elkhateeb Ghoson Daba Marwa Elnahas Paul Thomas Mahmoud Emam Metabolic profile and skin-related bioactivities of Cerioporus squamosus hydromethanolic extract
description	Abstract. Elkhateeb WA, Daba GM, Elnahas MO, Thomas PW, Emam M. 2020. Metabolic profile and skin-related bioactivities of Cerioporus squamosus hydromethanolic extract. Biodiversitas 21: 4732-4740. Being a functional food capable of showing nutritional as well as medicinal properties have great attention. Mushrooms have been proven as leading targets in this field. For this purpose, the edible mushroom Cerioporus squamosus was investigated in this study to evaluate the in vitro skin-related bioactivities of its hydromethanolic extract in terms of enhancing wound healing, and human skin cancer suppression capabilities. Treatment of fibroblast cells (BJ-1) with the hydromethanolic extract of this mushroom at 50 µg/mL enhanced cell migration rates by 71.7% after 24 h of exposure to the extract. Moreover, the same extract exhibited a promising impact on human skin cancer using an epidermoid carcinoma cell line (A431). The gradual increase in C. squamosus hydromethanolic extract concentration caused gradual decrease in the A431 cell viability and proliferation. Maximum effect on reducing the cell viability was obtained at a concentration of 100 µg/mL, where cell viability was 3.7%, and recorded IC50 was 52.6 µg/mL. The metabolic profile of the extract was analyzed by GC-MS, which was performed on its silylated metabolites. Nineteen compounds were detected including sugar alcohols, amino acids, fatty and organic acids. Promising results of this mushroom extract encourage conducting further steps towards using this mushroom as a functional food showing promising bioactivities.
format	article
author	Waill Elkhateeb Ghoson Daba Marwa Elnahas Paul Thomas Mahmoud Emam
author_facet	Waill Elkhateeb Ghoson Daba Marwa Elnahas Paul Thomas Mahmoud Emam
author_sort	Waill Elkhateeb
title	Metabolic profile and skin-related bioactivities of Cerioporus squamosus hydromethanolic extract
title_short	Metabolic profile and skin-related bioactivities of Cerioporus squamosus hydromethanolic extract
title_full	Metabolic profile and skin-related bioactivities of Cerioporus squamosus hydromethanolic extract
title_fullStr	Metabolic profile and skin-related bioactivities of Cerioporus squamosus hydromethanolic extract
title_full_unstemmed	Metabolic profile and skin-related bioactivities of Cerioporus squamosus hydromethanolic extract
title_sort	metabolic profile and skin-related bioactivities of cerioporus squamosus hydromethanolic extract
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/47e266075c3249d88386bc9dfa7cc19d
work_keys_str_mv	AT waillelkhateeb metabolicprofileandskinrelatedbioactivitiesofcerioporussquamosushydromethanolicextract AT ghosondaba metabolicprofileandskinrelatedbioactivitiesofcerioporussquamosushydromethanolicextract AT marwaelnahas metabolicprofileandskinrelatedbioactivitiesofcerioporussquamosushydromethanolicextract AT paulthomas metabolicprofileandskinrelatedbioactivitiesofcerioporussquamosushydromethanolicextract AT mahmoudemam metabolicprofileandskinrelatedbioactivitiesofcerioporussquamosushydromethanolicextract
_version_	1718418457457852416

Metabolic profile and skin-related bioactivities of Cerioporus squamosus hydromethanolic extract

Ejemplares similares